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Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development

2014-04Journal article Open Access
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dc.contributor.authorGuilgur, Leonardo Gastón
dc.contributor.authorPrudencio, Pedro
dc.contributor.authorSobral, Daniel
dc.contributor.authorLiszekova, Denisa
dc.contributor.authorRosa, Andre
dc.contributor.authorMartinho, Rui Goncalo
dc.date.accessioned2018-12-07T14:52:34Z
dc.date.available2018-12-07T14:52:34Z
dc.date.issued2014-04
dc.description.abstractDrosophila syncytial nuclear divisions limit transcription unit size of early zygotic genes. As mitosis inhibits not only transcription, but also pre-mRNA splicing, we reasoned that constraints on splicing were likely to exist in the early embryo, being splicing avoidance a possible explanation why most early zygotic genes are intronless. We isolated two mutant alleles for a subunit of the NTC/Prp19 complexes, which specifically impaired pre-mRNA splicing of early zygotic but not maternally encoded transcripts. We hypothesized that the requirements for pre-mRNA splicing efficiency were likely to vary during development. Ectopic maternal expression of an early zygotic pre-mRNA was sufficient to suppress its splicing defects in the mutant background. Furthermore, a small early zygotic transcript with multiple introns was poorly spliced in wild-type embryos. Our findings demonstrate for the first time the existence of a developmental pre-requisite for highly efficient splicing during Drosophila early embryonic development and suggest in highly proliferative tissues a need for coordination between cell cycle and gene architecture to ensure correct gene expression and avoid abnormally processed transcripts.
dc.description.sponsorshipFCT-Fundacao para a Ciencia e Tecnologia (Portugal) [PTDC/SAU-BID/111796/2009, PTDC/BIA-BCM/111822/ 2009, PTDC/BBB-BQB/0712/2012, PEst-OE/EQB/LA0023/2013, SFRH/BPD/47957/2008, SFRH/BPD/63869/2009]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.7554/eLife.02181
dc.identifier.issn2050-084X
dc.identifier.urihttp://hdl.handle.net/10400.1/11124
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElife Sciences Publications
dc.relationCharacterization of mesenchymal to epithelial transition in Drosophila melanogaster
dc.relationProtein targeting and N-terminal acetylation
dc.relationEXTRACELLULAR REGULATION OF EPITHELIAL CELL-CELL ADHESION DURING TISSUE MORPHOGENESIS
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSpliceosome activation
dc.subjectGene-expression
dc.subjectMitotic-cycles
dc.subjectCell-cycle
dc.subjectIn-vivo
dc.subjectTranscription
dc.subjectComplex
dc.subjectNuclear
dc.subjectReveals
dc.subjectEmbryogenesis
dc.titleRequirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCharacterization of mesenchymal to epithelial transition in Drosophila melanogaster
oaire.awardTitleProtein targeting and N-terminal acetylation
oaire.awardTitleEXTRACELLULAR REGULATION OF EPITHELIAL CELL-CELL ADHESION DURING TISSUE MORPHOGENESIS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-BID%2F111796%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-BQB%2F0712%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F47957%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F63869%2F2009/PT
oaire.citation.startPagee02181
oaire.citation.titleElife
oaire.citation.volume3
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameGuilgur
person.familyNamePrudêncio
person.familyNameVieira Noro e Silva Sobral
person.familyNameViegas Russo da Conceição Martinho
person.givenNameLeonardo Gastón
person.givenNamePedro
person.givenNameDaniel
person.givenNameRui Gonçalo
person.identifier.ciencia-id7B1C-9F36-22C8
person.identifier.ciencia-id5C13-14D8-F5A4
person.identifier.ciencia-idF31D-C783-3D89
person.identifier.orcid0000-0002-8197-5920
person.identifier.orcid0000-0003-1390-8491
person.identifier.orcid0000-0003-3955-0117
person.identifier.orcid0000-0002-1641-3403
person.identifier.scopus-author-id7801516140
person.identifier.scopus-author-id54884299400
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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