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Drosophila aPKC is required for mitotic spindle orientation during symmetric division of epithelial cells

2012-02Journal article Open access
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dc.contributor.authorGuilgur, Leonardo G.
dc.contributor.authorPrudencio, Pedro
dc.contributor.authorFerreira, Tania
dc.contributor.authorPimenta-Marques, Ana Rita
dc.contributor.authorGoncalo Martinho, Rui
dc.date.accessioned2018-12-07T14:53:00Z
dc.date.available2018-12-07T14:53:00Z
dc.date.issued2012-02
dc.description.abstractEpithelial cells mostly orient the spindle along the plane of the epithelium (planar orientation) for mitosis to produce two identical daughter cells. The correct orientation of the spindle relies on the interaction between cortical polarity components and astral microtubules. Recent studies in mammalian tissue culture cells suggest that the apically localised atypical protein kinase C (aPKC) is important for the planar orientation of the mitotic spindle in dividing epithelial cells. Yet, in chicken neuroepithelial cells, aPKC is not required in vivo for spindle orientation, and it has been proposed that the polarization cues vary between different epithelial cell types and/or developmental processes. In order to investigate whether Drosophila aPKC is required for spindle orientation during symmetric division of epithelial cells, we took advantage of a previously isolated temperature-sensitive allele of aPKC. We showed that Drosophila aPKC is required in vivo for spindle planar orientation and apical exclusion of Pins (Raps). This suggests that the cortical cues necessary for spindle orientation are not only conserved between Drosophila and mammalian cells, but are also similar to those required for spindle apicobasal orientation during asymmetric cell division.
dc.description.sponsorshipFundacao para Ciencia e Tecnologia [PTDC/SAU-BID/111796/2009, PTDC/QUI-BIQ/113027/2009, PTDC/BIA-BCM/111822/2009, SFRH/BPD/47957/2008, SFRH/BD/37587/2007, SFRH/BD/28767/2006]; Institute for Biotechnology and Bioengineering (IBB)/Centre for Molecular and Structural Biomedicine (CBME)
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1242/dev.071027
dc.identifier.issn0950-1991
dc.identifier.urihttp://hdl.handle.net/10400.1/11305
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCompany of Biologists
dc.relationCharacterization of mesenchymal to epithelial transition in Drosophila melanogaster
dc.relationAnalysis of the role of N-terminal acetyltransferases during Drosophila development.
dc.relationEXTRACELLULAR REGULATION OF EPITHELIAL CELL-CELL ADHESION DURING TISSUE MORPHOGENESIS
dc.relationDEFINING NOVEL MECHANISMS REQUIRED FOR THE FORMATION AND MORPHOGENESIS OF A POLARIZED EPITHELIUM
dc.relationDE NOVO FORMATION OF AN EPITHELIUM DURING DROSOPHILA BLASTODERM CELLULARIZATION: A GENETIC AND BIOCHEMICAL APPROACH
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectProtein-kinase-C
dc.subjectAsymmetric divisions
dc.subjectPolarity
dc.subjectNeuroblasts
dc.subjectCdc42
dc.subjectComplex
dc.subjectMechanisms
dc.subjectJunction
dc.subjectLgn
dc.subjectPhosphorylation
dc.titleDrosophila aPKC is required for mitotic spindle orientation during symmetric division of epithelial cells
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCharacterization of mesenchymal to epithelial transition in Drosophila melanogaster
oaire.awardTitleAnalysis of the role of N-terminal acetyltransferases during Drosophila development.
oaire.awardTitleEXTRACELLULAR REGULATION OF EPITHELIAL CELL-CELL ADHESION DURING TISSUE MORPHOGENESIS
oaire.awardTitleDEFINING NOVEL MECHANISMS REQUIRED FOR THE FORMATION AND MORPHOGENESIS OF A POLARIZED EPITHELIUM
oaire.awardTitleDE NOVO FORMATION OF AN EPITHELIUM DURING DROSOPHILA BLASTODERM CELLULARIZATION: A GENETIC AND BIOCHEMICAL APPROACH
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-BID%2F111796%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FQUI-BIQ%2F113027%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F47957%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F37587%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F28767%2F2006/PT
oaire.citation.endPage513
oaire.citation.issue3
oaire.citation.startPage503
oaire.citation.titleDevelopment
oaire.citation.volume139
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
person.familyNameViegas Russo da Conceição Martinho
person.givenNameRui Gonçalo
person.identifier.ciencia-idF31D-C783-3D89
person.identifier.orcid0000-0002-1641-3403
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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