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The recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosus across foci of canine leishmaniasis

2017-03Journal article Open access
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dc.contributor.authorKostalova, T.
dc.contributor.authorLestinova, T.
dc.contributor.authorMaia, C.
dc.contributor.authorSumova, P.
dc.contributor.authorVlkova, M.
dc.contributor.authorWillen, L.
dc.contributor.authorPolanska, N.
dc.contributor.authorFiorentino, E.
dc.contributor.authorScalone, A.
dc.contributor.authorOliva, G.
dc.contributor.authorVeronesi, F.
dc.contributor.authorCristovao, J. M.
dc.contributor.authorCourtenay, O.
dc.contributor.authorCampino, Lenea
dc.contributor.authorGradoni, L.
dc.contributor.authorGramiccia, M.
dc.contributor.authorVolf, P.
dc.date.accessioned2018-12-07T14:53:39Z
dc.date.available2018-12-07T14:53:39Z
dc.date.issued2017-03
dc.description.abstractThe frequency of sandfly-host contacts can be measured by host antibody levels against sandfly salivary proteins. Recombinant salivary proteins are suggested to represent a valid replacement for salivary gland homogenate (SGH); however, it is necessary to prove that such antigens are recognized by antibodies against various populations of the same species. Phlebotomus perniciosus (Diptera: Psychodidae) is the main vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in southwest Europe and is widespread from Portugal to Italy. In this study, sera were sampled from naturally exposed dogs from distant regions, including Campania (southern Italy), Umbria (central Italy) and the metropolitan Lisbon region (Portugal), where P. perniciosus is the unique or principal vector species. Sera were screened for anti-P. perniciosus antibodies using SGH and 43-kDa yellow-related recombinant protein (rSP03B). Arobust correlation between antibodies recognizing SGH and rSP03B was detected in all regions, suggesting substantial antigenic cross-reactivity among different P. perniciosus populations. No significant differences in this relationship were detected between regions. Moreover, rSP03B and the native yellow-related protein were shown to share similar antigenic epitopes, as canine immunoglobulin G (IgG) binding to the native protein was inhibited by pre-incubation with the recombinant form. These findings suggest that rSP03B should be regarded as a universal marker of sandfly exposure throughout the geographical distribution of P. perniciosus.
dc.description.sponsorshipCharles University [GAUK 1642314/2014]; European Union (EU) grant [FP7-261504]; EU's Horizon research and innovation programme under the Marie Sklodowska-Curie grant [642609]; Fundacao para a Ciencia e a Tecnologia [SFRH/BPD/44082/2008]; Ministerio da Educacao e Ciencia (Foundation for Science and Technology, Ministry of Education and Science), Portugal
dc.identifier.doi10.1111/mve.12192
dc.identifier.issn0269-283X
dc.identifier.issn1365-2915
dc.identifier.urihttp://hdl.handle.net/10400.1/11623
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relationDEVELOPMENT OF AN EXPERIMENTAL MODEL OF LEISHMANIA INFANTUM FOR THE EVALUATION OF VACCINE CANDIDATES
dc.relationControl of leishmaniasis, from bench to bedside and community
dc.subjectSalivary-Gland Proteins
dc.subjectVisceral Leishmaniasis
dc.subjectNested-Pcr
dc.subjectSand Flies
dc.subjectTransmission
dc.subjectVariability
dc.subjectSandflies
dc.subjectRisk
dc.subjectAge
dc.titleThe recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosus across foci of canine leishmaniasis
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEVELOPMENT OF AN EXPERIMENTAL MODEL OF LEISHMANIA INFANTUM FOR THE EVALUATION OF VACCINE CANDIDATES
oaire.awardTitleControl of leishmaniasis, from bench to bedside and community
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F44082%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/642609/EU
oaire.citation.endPage93
oaire.citation.issue1
oaire.citation.startPage88
oaire.citation.titleMedical and Veterinary Entomology
oaire.citation.volume31
oaire.fundingStreamH2020
person.familyNameCampino
person.givenNameLenea
person.identifier.ciencia-id2A16-58CC-48FE
person.identifier.orcid0000-0003-4512-9660
person.identifier.scopus-author-id6603808132
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication408b2ada-2070-4192-8710-aebcd7eba09a
relation.isAuthorOfPublication.latestForDiscovery408b2ada-2070-4192-8710-aebcd7eba09a
relation.isProjectOfPublication0efe32c2-1236-42f8-9e7f-5ee4ed9023ff
relation.isProjectOfPublication070d250d-4d64-4fda-bd24-45e22da0fc09
relation.isProjectOfPublication.latestForDiscovery070d250d-4d64-4fda-bd24-45e22da0fc09

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