Publication
Cerberus is a feedback inhibitor of Nodal asymmetric signaling in the chick embryo
| dc.contributor.author | Tavares, Ana Teresa | |
| dc.contributor.author | Andrade, Sofia | |
| dc.contributor.author | Silva, Ana Cristina | |
| dc.contributor.author | Belo, José A. | |
| dc.date.accessioned | 2018-12-07T14:53:34Z | |
| dc.date.available | 2018-12-07T14:53:34Z | |
| dc.date.issued | 2007-06 | |
| dc.description.abstract | The TGF-beta-related molecule Nodal plays an essential and conserved role in left-right patterning of the vertebrate embryo. Previous reports have shown that the zebrafish and mouse Cerberus-related proteins Charon and Cerberus-like-2 (Cerl-2), respectively, act in the node region to prevent the Nodal signal from crossing to the right side, whereas chick Cerberus (cCer) has an unclear function in the left-side mesoderm. In this study, we investigate the transcriptional regulation and function of cCer in left-right development. By analyzing the enhancer activity of cCer 5' genomic sequences in electroporated chick embryos, we identified a cCer left-side enhancer that contains two FoxH1 and one SMAD binding site. We show that these Nodal-responsive elements are necessary and sufficient for the activation of transcription in the left-side mesoderm. In transgenic mouse embryos, cCer regulatory sequences behave as in chick embryos, suggesting that the cis-regulatory sequences of Cerberus-related genes have diverged during vertebrate evolution. Moreover, our findings from cCer overexpression and knockdown experiments indicate that cCer is a negative-feedback regulator of Nodal asymmetric signaling. We propose that cCer and mouse Cerl-2 have evolved distinct regulatory mechanisms but retained a conserved function in left-right development, which is to restrict Nodal activity to the left side of the embryo. | |
| dc.description.version | info:eu-repo/semantics/publishedVersion | |
| dc.identifier.doi | 10.1242/dev.000901 | |
| dc.identifier.issn | 0950-1991 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/11578 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Company Of Biologists Ltd | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Early mouse embryo | |
| dc.subject | Vertebrate development | |
| dc.subject | Primitive streak | |
| dc.subject | Gene-expression | |
| dc.subject | Secreted factor | |
| dc.subject | Axis formation | |
| dc.subject | Lateral plate | |
| dc.subject | Tgf-Beta | |
| dc.subject | Bmp | |
| dc.subject | Antagonist | |
| dc.title | Cerberus is a feedback inhibitor of Nodal asymmetric signaling in the chick embryo | |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 2060 | |
| oaire.citation.issue | 11 | |
| oaire.citation.startPage | 2051 | |
| oaire.citation.title | Development | |
| oaire.citation.volume | 134 | |
| person.familyName | Belo | |
| person.givenName | José A. | |
| person.identifier.ciencia-id | BF13-08E9-25E6 | |
| person.identifier.orcid | 0000-0001-7384-0949 | |
| person.identifier.rid | F-4444-2012 | |
| person.identifier.scopus-author-id | 6602141392 | |
| rcaap.rights | openAccess | |
| rcaap.type | article | |
| relation.isAuthorOfPublication | 4bebeb48-6fac-41a9-8363-f849def5e019 | |
| relation.isAuthorOfPublication.latestForDiscovery | 4bebeb48-6fac-41a9-8363-f849def5e019 |
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