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Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development

dc.contributor.authorFurtado, João
dc.contributor.authorBento, Margaret
dc.contributor.authorCorreia, Elizabeth
dc.contributor.authorInacio, Jose Manuel
dc.contributor.authorBelo, José A.
dc.date.accessioned2018-12-07T14:52:54Z
dc.date.available2018-12-07T14:52:54Z
dc.date.issued2014-12
dc.description.abstractDuring the course of a differential screen to identify transcripts specific for chick heart/hemangioblast precursor cells, we have identified Ccbe1 (Collagen and calcium-binding EGF-like domain 1). While the importance of Ccbe1 for the development of the lymphatic system is now well demonstrated, its role in cardiac formation remained unknown. Here we show by whole-mount in situ hybridization analysis that cCcbe1 mRNA is initially detected in early cardiac progenitors of the two bilateral cardiogenic fields (HH4), and at later stages on the second heart field (HH9-18). Furthermore, cCcbe1 is expressed in multipotent and highly proliferative cardiac progenitors. We characterized the role of cCcbe1 during early cardiogenesis by performing functional studies. Upon morpholino-induced cCcbe1 knockdown, the chick embryos displayed heart malformations, which include aberrant fusion of the heart fields, leading to incomplete terminal differentiation of the cardiomyocytes. cCcbe1 overexpression also resulted in severe heart defects, including cardia bifida. Altogether, our data demonstrate that although cardiac progenitors cells are specified in cCcbe1 morphants, the migration and proliferation of cardiac precursors cells are impaired, suggesting that cCcbe1 is a key gene during early heart development.
dc.description.sponsorshipFCT [SFRH/BD/65628/2009, SFRH/BPD/86497/2012, SFRH/BPD/41081/2007]; F.C.T.B.I. fellowship [PTDC/SAU-BID/114902/ 2009]; FCT; Institute for Biotechnology Bioengineering (Centro Biomedicina Molecular e Celular (IBB/CBME), Laboratorio Associado (LA) in the frame of Project [PestOE/EQB/LA0023/2013]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1371/journal.pone.0115481
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/11260
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCardiac progenitor cells
dc.subjectAvian precardiac cells
dc.subjectNeural crest
dc.subjectExtracellular-matrix
dc.subjectTranscription factor
dc.subjectVentral morphogenesis
dc.subjectMovement patterns
dc.subjectConduction system
dc.subjectTube formation
dc.subjectMigration
dc.titleExpression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F86497%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F41081%2F2007/PT
oaire.citation.issue12
oaire.citation.startPagee115481
oaire.citation.titlePLoS ONE
oaire.citation.volume9
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
person.familyNameFurtado
person.familyNameBento Soares
person.familyNameInacio
person.familyNameBelo
person.givenNameJoão
person.givenNameMargaret
person.givenNameJose M
person.givenNameJosé A.
person.identifier.ciencia-id811B-BD33-0000
person.identifier.ciencia-idD914-5A0B-3F42
person.identifier.ciencia-idB810-3281-8D8C
person.identifier.ciencia-idBF13-08E9-25E6
person.identifier.orcid0000-0003-0689-1978
person.identifier.orcid0000-0002-9790-8335
person.identifier.orcid0000-0001-7384-0949
person.identifier.ridO-1455-2018
person.identifier.ridF-4444-2012
person.identifier.scopus-author-id6604046494
person.identifier.scopus-author-id6602141392
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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