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NineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal-ventral patterning

2020-12Journal article Restricted access
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dc.contributor.authorRathore, Om
dc.contributor.authorSilva, Rui D.
dc.contributor.authorAscensao-Ferreira, Mariana
dc.contributor.authorMatos, Ricardo
dc.contributor.authorCarvalho, Celia
dc.contributor.authorMarques, Bruno
dc.contributor.authorTiago, Margarida N.
dc.contributor.authorPrudencio, Pedro
dc.contributor.authorAndrade, Raquel P.
dc.contributor.authorRoignant, Jean-Yves
dc.contributor.authorBarbosa-Morais, Nuno
dc.contributor.authorMartinho, Rui Goncalo
dc.date.accessioned2021-06-18T16:25:44Z
dc.date.available2021-06-18T16:25:44Z
dc.date.issued2020-12
dc.description.abstractThe NineTeen Complex (NTC), also known as pre-mRNA-processing factor 19 (Prp19) complex, regulates distinct spliceosome conformational changes necessary for splicing. During Drosophila midblastula transition, splicing is particularly sensitive to mutations in NTC-subunit Fandango, which suggests differential requirements of NTC during development. We show that NTC-subunit Salsa, the Drosophila ortholog of human RNA helicase Aquarius, is rate-limiting for splicing of a subset of small first introns during oogenesis, including the first intron of gurken. Germline depletion of Salsa and splice site mutations within gurken first intron impair both adult female fertility and oocyte dorsal-ventral patterning, due to an abnormal expression of Gurken. Supporting causality, the fertility and dorsal-ventral patterning defects observed after Salsa depletion could be suppressed by the expression of a gurken construct without its first intron. Altogether, our results suggest that one of the key rate-limiting functions of Salsa during oogenesis is to ensure the correct expression and efficient splicing of the first intron of gurken mRNA. Retention of gurken first intron compromises the function of this gene most likely because it undermines the correct structure and function of the transcript 5'UTR.
dc.description.sponsorshipPortuguese national funding [FCT: PPBI-POCI-01-0145-FEDER-022122]
dc.description.sponsorshipResearch infrastructure Congento [LISBOA-01-0145-FEDER-022170]
dc.description.sponsorshipPortuguese national funding through Fundacao para a Ciencia e a Tecnologia (FCT) [CBMR 1334]
dc.description.sponsorshipEuropean Molecular Biology Organization (EMBO) Installation GrantEuropean Molecular Biology Organization (EMBO) [3057]
dc.description.sponsorshipFundacao para a Ciencia e a Tecnologia (FCT)/Ministerio da Ciencia, Tecnologia e Ensino Superior (MCTES) through Fundos do Orcamento de Estado
dc.description.sponsorshipIMM Lisboa start-up funds
dc.description.sponsorshipPortuguese national funding through Fundacao para a Ciencia e a Tecnologia [PD/BD/52428/2013, PD/BD/128342/2017, PD/00117/2012, CRM:0027030, 57/2016/CP1361/CT0019]
dc.description.sponsorshipFederation of European Biochemical societies (FEBS) short-term fellowship
dc.description.sponsorshipFundacao AstraZeneca
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1261/rna.077446.120
dc.identifier.issn1355-8382
dc.identifier.urihttp://hdl.handle.net/10400.1/15659
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCold Spring Harbor Laboratory Press
dc.subjectSplicing
dc.subjectDrosophila
dc.subjectFemale fertility
dc.subjectDorsal-ventral patterning
dc.subjectGurken
dc.subject.otherBiochemistry & Molecular Biology
dc.titleNineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal-ventral patterning
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/138992/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/149670/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04773%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/59820/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCTI/2064/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/154779/PT
oaire.citation.endPage1956
oaire.citation.issue12
oaire.citation.startPage1935
oaire.citation.titleRNA
oaire.citation.volume26
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream5876
oaire.fundingStreamOE
oaire.fundingStreamPOCTI
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRathore
person.familyNameSilva
person.familyNamePrudêncio
person.familyNameBarbosa-Morais
person.givenNameOm
person.givenNameRui
person.givenNamePedro
person.givenNameNuno
person.identifier.ciencia-id7B1C-9F36-22C8
person.identifier.ciencia-id5C19-CA77-A74D
person.identifier.orcid0000-0003-3858-557X
person.identifier.orcid0000-0003-2168-8599
person.identifier.orcid0000-0003-1390-8491
person.identifier.orcid0000-0002-1215-0538
person.identifier.ridM-7546-2013
person.identifier.ridC-5622-2012
person.identifier.ridI-2743-2013
person.identifier.scopus-author-id15045741700
person.identifier.scopus-author-id54884299400
person.identifier.scopus-author-id6507555084
project.funder.identifierhttp://doi.org/10.13039/501100001871
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rcaap.rightsrestrictedAccess
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