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Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics

2020-05Journal article Open Access
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dc.contributor.authorSantos, Ana Isabel
dc.contributor.authorLourenco, Ana S.
dc.contributor.authorSimão, Sónia
dc.contributor.authorMarques-da-Silva, Dorinda
dc.contributor.authorSantos, Daniela F.
dc.contributor.authorCarvalho, Ana Paula Onofre de
dc.contributor.authorPereira, Ana Catarina
dc.contributor.authorIzquierdo-Álvarez, Alicia
dc.contributor.authorRamos, Elena
dc.contributor.authorMorato, Esperanza
dc.contributor.authorMarina, Anabel
dc.contributor.authorMartínez-Ruiz, Antonio
dc.contributor.authorAraújo, Inês
dc.date.accessioned2020-06-22T11:13:43Z
dc.date.available2020-06-22T11:13:43Z
dc.date.issued2020-05
dc.description.abstractNitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R-SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC.pt_PT
dc.description.sponsorshipPTDC/QUI-QFI/29319/2017; UID/BIM/04773/2019pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.redox.2020.101457pt_PT
dc.identifier.issn2213-2317
dc.identifier.urihttp://hdl.handle.net/10400.1/14045
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationENHANCEMENT OF POST-INJURY NEUROGENESIS BY ANTI-INFLAMMATORY DRUGS AND NITRIC OXIDE
dc.relationEnhancement of endogenous neurogenesis by nitric oxide: identification of S-nitrosylation targets
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNitric oxidept_PT
dc.subjectHippocampuspt_PT
dc.subjectS-nitrosylationpt_PT
dc.subjectSeizurespt_PT
dc.subjectNeural stem cellspt_PT
dc.subjectNeurogenesispt_PT
dc.titleIdentification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomicspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleENHANCEMENT OF POST-INJURY NEUROGENESIS BY ANTI-INFLAMMATORY DRUGS AND NITRIC OXIDE
oaire.awardTitleEnhancement of endogenous neurogenesis by nitric oxide: identification of S-nitrosylation targets
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F77903%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F79308%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FNEU-OSD%2F0473%2F2012/PT
oaire.citation.startPage101457pt_PT
oaire.citation.titleRedox Biologypt_PT
oaire.citation.volume32pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
person.familyNameSantos
person.familyNameLourenço
person.familyNameMarques da Silva
person.familyNameFilipa Santos
person.familyNamePombinho de Araújo
person.givenNameAna Isabel
person.givenNameAna Sofia
person.givenNameDorinda
person.givenNameDaniela
person.givenNameInês Maria
person.identifier170713
person.identifierF-4703-2012
person.identifier.ciencia-idA31D-E831-28E8
person.identifier.ciencia-id521A-35C1-078D
person.identifier.ciencia-id7917-DC57-F21B
person.identifier.ciencia-idD11F-D4CA-2947
person.identifier.orcid0000-0003-2070-0285
person.identifier.orcid0000-0003-2882-6026
person.identifier.orcid0000-0003-0460-5421
person.identifier.orcid0000-0001-6855-1763
person.identifier.orcid0000-0002-2438-0111
person.identifier.scopus-author-id55019179900
person.identifier.scopus-author-id56822397300
person.identifier.scopus-author-id56271084100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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