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Cell–fibronectin interactions and actomyosin contractility regulate the segmentation clock and spatio-temporal somite cleft formation during chick embryo somitogenesis

2022-06-22Journal article Open Access
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dc.contributor.authorGomes De Almeida, Patrícia
dc.contributor.authorRifes, Pedro
dc.contributor.authorJesus, Ana Patrícia
dc.contributor.authorPinheiro, Gonçalo
dc.contributor.authorP. Andrade, Raquel
dc.contributor.authorThorsteinsdóttir, Sólveig
dc.date.accessioned2022-07-14T12:28:05Z
dc.date.available2022-07-14T12:28:05Z
dc.date.issued2022-06-22
dc.date.updated2022-07-08T11:55:08Z
dc.description.abstractFibronectin is essential for somite formation in the vertebrate embryo. Fibronectin matrix assembly starts as cells emerge from the primitive streak and ingress in the unsegmented presomitic mesoderm (PSM). PSM cells undergo cyclic waves of segmentation clock gene expression, followed by Notch-dependent upregulation of meso1 in the rostral PSM which induces somite cleft formation. However, the relevance of the fibronectin matrix for these molecular processes remains unknown. Here, we assessed the role of the PSM fibronectin matrix in the spatio-temporal regulation of chick embryo somitogenesis by perturbing (1) extracellular fibronectin matrix assembly, (2) integrin–fibronectin binding, (3) Rho-associated protein kinase (ROCK) activity and (4) non-muscle myosin II (NM II) function. We found that integrin–fibronectin engagement and NM II activity are required for cell polarization in the nascent somite. All treatments resulted in defective somitic clefts and significantly perturbed meso1 and segmentation clock gene expression in the PSM. Importantly, inhibition of actomyosin-mediated contractility increased the period of hairy1/hes4 oscillations from 90 to 120 min. Together, our work strongly suggests that the fibronectin–integrin–ROCK–NM II axis regulates segmentation clock dynamics and dictates the spatio-temporal localization of somitic clefts.pt_PT
dc.description.sponsorshipPPBI-POCI-01-0145-FEDER-022122
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifierdoi: 10.3390/cells11132003
dc.identifier.citationCells 11 (13): 2003 (2022)pt_PT
dc.identifier.doi10.3390/cells11132003pt_PT
dc.identifier.eissn2073-4409
dc.identifier.urihttp://hdl.handle.net/10400.1/17999
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationChanging extracellular matrix networks as regulators of transitions between epithelial and mesenchymal fates during embryogenesis.
dc.relationTIMING CELL DIFFERENTIATON: Gene Regulatory Network in Temporal Control of Mesoderm Specification
dc.relationNot Available
dc.relationCentre for Ecology, Evolution and Environmental Changes
dc.relationCentre for Biomedical Research
dc.relationCOORDINATING SIGNALING PATHWAYS IN DEVELOPMENT: CROSS-TALK BETWEEN SONIC HEDGEHOG AND THE EXTRACELLULAR MATRIX IN SOMITOGENESIS
dc.relationFIBRONECTIN-INTEGRIN CUES IN SOMITE FORMATION
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectFibronectinpt_PT
dc.subjectFibronectin matrix assemblypt_PT
dc.subjectActomyosin contractilitypt_PT
dc.subjectSomitogenesispt_PT
dc.subjectSegmentation clockpt_PT
dc.subjectHairy1pt_PT
dc.subjectCleft formationpt_PT
dc.titleCell–fibronectin interactions and actomyosin contractility regulate the segmentation clock and spatio-temporal somite cleft formation during chick embryo somitogenesispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleChanging extracellular matrix networks as regulators of transitions between epithelial and mesenchymal fates during embryogenesis.
oaire.awardTitleTIMING CELL DIFFERENTIATON: Gene Regulatory Network in Temporal Control of Mesoderm Specification
oaire.awardTitleNot Available
oaire.awardTitleCentre for Ecology, Evolution and Environmental Changes
oaire.awardTitleCentre for Biomedical Research
oaire.awardTitleCOORDINATING SIGNALING PATHWAYS IN DEVELOPMENT: CROSS-TALK BETWEEN SONIC HEDGEHOG AND THE EXTRACELLULAR MATRIX IN SOMITOGENESIS
oaire.awardTitleFIBRONECTIN-INTEGRIN CUES IN SOMITE FORMATION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-OBD%2F103771%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBEX-BID%2F5410%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F01589%2F2017%2FCP1387%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00329%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F04773%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F86980%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PIDDAC/SFRH%2FBD%2F37423%2F2007/PT
oaire.citation.issue13pt_PT
oaire.citation.startPage2003pt_PT
oaire.citation.titleCellspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamCEEC IND 2017
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPIDDAC
person.familyNameGomes de Almeida
person.familyNameJesus
person.familyNameGoulart da Silva Pinheiro
person.familyNameAndrade
person.givenNamePatrícia
person.givenNameAna Patrícia
person.givenNameGonçalo
person.givenNameRaquel
person.identifier.ciencia-idC91E-9CCB-8C43
person.identifier.ciencia-idFC1A-C7E5-EE1A
person.identifier.ciencia-id2312-360B-227A
person.identifier.orcid0000-0003-2369-2874
person.identifier.orcid0000-0003-1598-6397
person.identifier.orcid0000-0002-0397-5917
person.identifier.scopus-author-id7103114918
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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