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Clinical performance of an infliximab rapid quantification assay

2017-09Journal article Open access
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dc.contributor.authorMagro, Fernando
dc.contributor.authorAfonso, Joana
dc.contributor.authorLopes, Susana
dc.contributor.authorCoelho, Rosa
dc.contributor.authorGonçalves, Raquel
dc.contributor.authorCaldeira, Paulo
dc.contributor.authorLago, Paula
dc.contributor.authorSousa, Helena Tavares
dc.contributor.authorRamos, Jaime
dc.contributor.authorGonçalves, Ana Rita
dc.contributor.authorMinistro, Paula
dc.contributor.authorRosa, Isadora
dc.contributor.authorMeira, Tania
dc.contributor.authorAndrade, PatrĂ­cia
dc.contributor.authorSoares, JoĂŁo-Bruno
dc.contributor.authorCarvalho, Diana
dc.contributor.authorSousa, Paula
dc.contributor.authorVieira, Ana Isabel
dc.contributor.authorLopes, Joanne
dc.contributor.authorDias, ClĂĄudia Camila
dc.contributor.authorGeboes, Karel
dc.contributor.authorCarneiro, FĂĄtima
dc.date.accessioned2018-12-07T14:58:11Z
dc.date.available2018-12-07T14:58:11Z
dc.date.issued2017-09
dc.description.abstractBackground: Therapeutic drug monitoring (TDM)-based algorithms can be used to guide infliximab (IFX) adjustments in inflammatory bowel disease (IBD) patients. This study aimed to explore a rapid IFX-quantification test from a clinical perspective. Methods: This manuscript describes a prospective cohort study involving 110 ulcerative colitis (UC) patients on the maintenance phase of IFX. IFX trough levels were quantified using a rapid quantification assay and a commonly-used reference kit. Results: Irrespective of the assay used to measure IFX, its through levels were statistically different between patients with and without endoscopic remission (Mayo endoscopic score = 0), as well as between patients stratified by their faecal calprotectin (FC) levels. Despite the fact that the two methods correlated well with each other [Spearman's rank correlation coefficient = 0.843, p < 0.001; intraclass correlation coefficients = 0.857, 95% confidence interval (CI): 0.791-0.903], there was a discernible systematic variation; values obtained with the reference kit were on average 2.62 units higher than those obtained with the rapid assay. Notwithstanding, 3 mu g/ml was shown to be an acceptable cut-off to assess endoscopic status and inflammatory burden levels using both assays. The percentage of patients that had a positive outcome when the IFX concentration measured by the rapid assay ranked above 3 mu g/ml was 88% both for a Mayo endoscopic score <= 1 and for an FC concentration <250 mu g/g. Conclusions: Based on this study, we concluded that using the rapid IFX assessment system with a 3 mu g/ml threshold is a reliable alternative to the time-consuming enzyme-linked immunosorbent assays in patients on the maintenance phase of IFX.
dc.description.sponsorshipPortuguese IBD Group (GEDII, Grupo de Estudo da Doenca Inflamatoria Intestinal)
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1177/1756283X17722916
dc.identifier.issn1756-283X
dc.identifier.issn1756-2848
dc.identifier.urihttp://hdl.handle.net/10400.1/11905
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSage Publications Ltd
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectInflammatory-bowel-disease
dc.subjectUlcerative-colitis
dc.subjectCrohns-disease
dc.subjectSerum infliximab
dc.subjectElisa Kits
dc.subjectAntibodies
dc.subjectTherapy
dc.subjectOptimization
dc.subjectAssociation
dc.subjectCohort
dc.titleClinical performance of an infliximab rapid quantification assay
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage660
oaire.citation.issue9
oaire.citation.startPage651
oaire.citation.titleTherapeutic Advances in Gastroenterology
oaire.citation.volume10
person.familyNameCaldeira
person.familyNameSousa
person.givenNamePaulo
person.givenNameHelena Tavares
person.identifier.ciencia-id1112-F0B2-19A3
person.identifier.ciencia-idCF1F-1163-1C4A
person.identifier.orcid0000-0002-2404-6295
person.identifier.orcid0000-0002-6626-205X
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication3def3720-8982-4c46-801d-af640d508b59
relation.isAuthorOfPublication6b1d11dd-486f-4fb3-b41f-02e1cf6a5c2e
relation.isAuthorOfPublication.latestForDiscovery6b1d11dd-486f-4fb3-b41f-02e1cf6a5c2e

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