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Human Mob1 proteins are required for cytokinesis by controlling microtubule stability

2012-07Journal article Open access
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dc.contributor.authorFlorindo, Claudia
dc.contributor.authorPerdigao, Joana
dc.contributor.authorFesquet, Didier
dc.contributor.authorSchiebel, Elmar
dc.contributor.authorPines, Jonathon
dc.contributor.authorTavares, Alvaro A.
dc.date.accessioned2018-12-07T14:53:20Z
dc.date.available2018-12-07T14:53:20Z
dc.date.issued2012-07
dc.description.abstractThe completion of cytokinesis requires abscission of the midbody, a microtubule-rich cytoplasmic bridge that connects the daughter cells before their final separation. Although it has been established that both the midbody structure and membrane fusion are essential for abscission, the biochemical machinery and the cellular processes of abscission remain ill-defined. Here we report that human Mob1A and Mob1B proteins are involved in the regulation of abscission of the intercellular bridge. The Mob family is a group of highly conserved proteins in eukaryotes, described as binding partners as well as co-activators of protein kinases of the Ndr family, and as members of the Hippo pathway. We show that depletion of Mob1A and Mob1B by RNAi causes abscission failure as a consequence of hyper-stabilization of microtubules in the midbody region. Interestingly, depleting Mob1 also increases cell motility after cytokinesis, and induces prolonged centriole separation in G1 phase. In contrast, centrosomes fail to split when either Mob1A or Mob1B is overexpressed. Our findings indicate that human Mob1 proteins are involved in the regulation of microtubule stability at the midbody. We conclude that Mob1A and Mob1B are needed for cell abscission and centriole re-joining after telophase and cytokinesis.
dc.description.sponsorshipFCT [PRAXIS XXI/DB/19823/99, SFRH/BPD/20698/2004, POCTI/BCI/34405/99, POCTI/CBO/39099/2001, POCTI/BIA/PRO/60337/2004, PTDC/SAU-OBD/105234-2008, PEst-OE/EQB/LA0023/2011]; Contract ARC [5479]; Medical Research Council [G1000818]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1242/jcs.097147
dc.identifier.issn0021-9533
dc.identifier.urihttp://hdl.handle.net/10400.1/11466
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCompany Of Biologists Ltd
dc.relationDEFINING THE ROLE OF HUMAN MOB PROTEINS IN CELL CYCLE CONTROL AND TUMUROGENISIS
dc.relationStructure and function of the centrosomal proteins HsMob in cell division
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTumor-Suppressor
dc.subjectMitotic exit
dc.subjectCell-proliferation
dc.subjectFurrow formation
dc.subjectMammalian-cells
dc.subjectKinase
dc.subjectCentrosome
dc.subjectDrosophila
dc.subjectEscrt
dc.subjectAbscission
dc.titleHuman Mob1 proteins are required for cytokinesis by controlling microtubule stability
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDEFINING THE ROLE OF HUMAN MOB PROTEINS IN CELL CYCLE CONTROL AND TUMUROGENISIS
oaire.awardTitleStructure and function of the centrosomal proteins HsMob in cell division
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F20698%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCTI%2FCBO%2F39099%2F2001/PT
oaire.citation.endPage3090
oaire.citation.issue13
oaire.citation.startPage3085
oaire.citation.titleJournal of Cell Science
oaire.citation.volume125
oaire.fundingStreamPOCI
person.familyNameFlorindo
person.familyNameTavares
person.givenNameClaudia
person.givenNameAlvaro
person.identifier.ciencia-id7812-FEB4-622A
person.identifier.ciencia-idAD12-B7F1-969B
person.identifier.orcid0000-0003-4549-2179
person.identifier.orcid0000-0002-7137-0944
person.identifier.scopus-author-id26531090300
person.identifier.scopus-author-id19036533500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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relation.isAuthorOfPublicationb44cba91-e2b1-4439-ba25-6ec1d68a059c
relation.isAuthorOfPublication.latestForDiscovery4444e021-d38a-470f-b2d7-35fd2d7c1883
relation.isProjectOfPublicationbf1a8a21-8d20-4276-9b00-95588513fe76
relation.isProjectOfPublication5e849433-be2b-4b1d-826e-dd4342292c7f
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