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The embryonic Brachyury transcription factor is a novel biomarker of GIST aggressiveness and poor survival

2016-04Journal article Restricted access
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dc.contributor.authorPinto, Filipe
dc.contributor.authorCampanella, Nathalia C.
dc.contributor.authorAbrahao-Machado, Lucas F.
dc.contributor.authorScapulatempo-Neto, Cristovam
dc.contributor.authorde Oliveira, Antonio T.
dc.contributor.authorBrito, Maria J.
dc.contributor.authorAndrade, Raquel P.
dc.contributor.authorGuimaraes, Denise P.
dc.contributor.authorReis, Rui M.
dc.date.accessioned2017-04-07T15:56:52Z
dc.date.available2017-04-07T15:56:52Z
dc.date.issued2016-04
dc.description.abstractThe T-box transcription factor Brachyury was recently reported to be upregulated and associated with prognosis in solid tumors. Here, we proposed to evaluate the potential use of Brachyury protein expression as a new prognostic biomarker in gastrointestinal stromal tumors (GIST).Brachyury protein expression was analyzed by immunohistochemistry in a cohort of 63 bona fide GIST patients. Brachyury expression profiles were correlated with patients' clinicopathological features and prognostic impact. Additionally, an in silico analysis was performed using the Oncomine database to assess Brachyury alterations at DNA and mRNA levels in GISTs.We found that Brachyury was overexpressed in the majority (81.0 %) of primary GISTs. We observed Brachyury staining in the nucleus alone in 4.8 % of cases, 23.8 % depicted only cytoplasm staining, and 52.4 % of cases exhibited both nucleus and cytoplasm immunostaining. The presence of Brachyury was associated with aggressive GIST clinicopathological features. Particularly, Brachyury nuclear (with or without cytoplasm) staining was associated with the presence of metastasis, while cytoplasm sublocalization alone was correlated with poor patient survival.Herein, we demonstrate that Brachyury is overexpressed in GISTs and is associated with worse outcome, constituting a novel prognostic biomarker and a putative target for GIST treatment.
dc.description.sponsorshipCNPq Universal (476192/2013-7) for RMR; NCC is a recipient of an FAPESP doctoral fellowship (2013/25787-3)
dc.identifier.doi10.1007/s10120-015-0505-0
dc.identifier.issn1436-3291
dc.identifier.urihttp://hdl.handle.net/10400.1/9547
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer Verlag
dc.relationLEARNING FROM THE EMBRYO: UNDERSTANDING EARLY EMBRYONIC T GENE – BRACHYURY - IMPACT IN HUMAN TUMORIGENESIS
dc.relation.isbasedonWOS:000373745700038
dc.titleThe embryonic Brachyury transcription factor is a novel biomarker of GIST aggressiveness and poor survival
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleLEARNING FROM THE EMBRYO: UNDERSTANDING EARLY EMBRYONIC T GENE – BRACHYURY - IMPACT IN HUMAN TUMORIGENESIS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F81369%2F2011/PT
oaire.citation.endPage659
oaire.citation.issue2
oaire.citation.startPage651
oaire.citation.titleGastric Cancer
oaire.citation.volume19
person.familyNameAndrade
person.givenNameRaquel
person.identifier.ciencia-id2312-360B-227A
person.identifier.orcid0000-0002-0397-5917
person.identifier.scopus-author-id7103114918
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscoveryf2d3fc39-a8f6-4d83-9e60-2e2fdfbc2b4a
relation.isProjectOfPublication5e84962a-5632-46bf-94b9-20742b991dd9
relation.isProjectOfPublication.latestForDiscovery5e84962a-5632-46bf-94b9-20742b991dd9

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