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Endogenous Cerberus activity is required for anterior head specification in Xenopus

2003-10Journal article Open Access
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dc.contributor.authorSilva, Ana Cristina
dc.contributor.authorFilipe, Mário
dc.contributor.authorKuerner, Klaus-Michael
dc.contributor.authorSteinbeisser, Herbert
dc.contributor.authorBelo, José A.
dc.date.accessioned2018-12-07T14:58:05Z
dc.date.available2018-12-07T14:58:05Z
dc.date.issued2003-10
dc.description.abstractWe analyzed the endogenous requirement for Cerberus in Xenopus head development. 'Knockdown' of Cerberus function by antisense morpholino oligonucleotides did not impair head formation in the embryo. In contrast, targeted increase of BMP, Nodal and Wnt signaling in the anterior dorsal-endoderm (ADE) resulted in synergistic loss of anterior head structures, without affecting more posterior axial ones. Remarkably, those head phenotypes were aggravated by simultaneous depletion of Cerberus. These experiments demonstrated for the first time that endogenous Cerberus protein can inhibit BMP, Nodal and Wnt factors in vivo. Conjugates of dorsal ectoderm (DE) and ADE explants in which Cerberus function was 'knocked down' revealed the requirement of Cerberus in the ADE for the proper induction of anterior neural markers and repression of more posterior ones. This data supports the view that Cerberus function is required in the leading edge of the ADE for correct induction and patterning of the neuroectoderm.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doihttps://doi.org/10.1242/dev.00705
dc.identifier.issn0950-1991
dc.identifier.urihttp://hdl.handle.net/10400.1/11850
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCompany of Biologists
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSpemann organizer
dc.subjectVisceral endoderm
dc.subjectTranscription factor
dc.subjectPrimitive endoderm
dc.subjectSecreted proteins
dc.subjectGene
dc.subjectExpression
dc.subjectInduction
dc.subjectMouse
dc.subjectGastrulation
dc.subjectCerberus
dc.subjectHead induction
dc.subjectMorpholino
dc.subjectTargeted activation
dc.subjectXenopus laevis
dc.titleEndogenous Cerberus activity is required for anterior head specification in Xenopus
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage4953
oaire.citation.issue20
oaire.citation.startPage4943
oaire.citation.titleDevelopment
oaire.citation.volume130
person.familyNameBelo
person.givenNameJosé A.
person.identifier.ciencia-idBF13-08E9-25E6
person.identifier.orcid0000-0001-7384-0949
person.identifier.ridF-4444-2012
person.identifier.scopus-author-id6602141392
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication4bebeb48-6fac-41a9-8363-f849def5e019
relation.isAuthorOfPublication.latestForDiscovery4bebeb48-6fac-41a9-8363-f849def5e019

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