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Methylation patterns in dysplasia in inflammatory bowel disease patients

2020-06Journal article Restricted access
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dc.contributor.authorRosa, Isadora
dc.contributor.authorSilva, Patricia
dc.contributor.authorda Mata, Sara
dc.contributor.authorMagro, Fernando
dc.contributor.authorCarneiro, Fatima
dc.contributor.authorPeixoto, Armando
dc.contributor.authorSilva, Marco
dc.contributor.authorSousa, Helena Tavares
dc.contributor.authorRoseira, Joana
dc.contributor.authorParra, Jose
dc.contributor.authorBarosa, Rita
dc.contributor.authorVieira, Ana
dc.contributor.authorBrito, Maria Jos
dc.contributor.authorLago, Paula
dc.contributor.authorCoelho, Andre
dc.contributor.authorMoleiro, Joana
dc.contributor.authorda Silva, Joao Pereira
dc.contributor.authorFonseca, Ricardo
dc.contributor.authorAlbuquerque, Cristina
dc.contributor.authorDias Pereira, A.
dc.date.accessioned2021-06-24T11:35:34Z
dc.date.available2021-06-24T11:35:34Z
dc.date.issued2020-06
dc.description.abstractBackground and aims:Inflammatory Bowel Disease (IBD) with colonic involvement increases colorectal cancer risk. However, the distinction between IBD related and sporadic dysplasia in IBD patients is difficult. Some data favors the importance of abnormal DNA methylation in IBD-related carcinogenesis. We aimed to define methylation patterns in patients with colonic cancer or dysplasia diagnosis following an IBD diagnosis. Methods:Multicentric cross-sectional study-91 samples from colonic mucosa with/without dysplasia from 9 patients with IBD-related dysplasia/cancer and 26 patients with IBD and sporadic dysplasia/cancer were included. Methylation patterns of CpG islands in the promoter regions of 67 genes were studied by Methylation-specific Multiplex Ligation-dependent Probe Amplification. Results:Mean age at IBD diagnosis: 42 +/- 16 years;at dysplasia diagnosis: 56 +/- 14 years. Twenty-ninepatients had ulcerative colitis. Twenty-five patients had at least 1 lesion endoscopically described as adenoma-like, 4 at least 1 non-adenoma like, 3 had cancer and 3 had dysplasia in flat mucosa. No patient had both adenoma-like and non-adenoma-like lesions. Patients with an IBD-related lesion were significantly younger at IBD diagnosis (p = .003) and at dysplasia/cancer diagnosis (p = .039). Promoter methylation ofIGF2, RARB, ESR1, CHFR, CDH13, WT1, GATA5, WIF1genes was significantly associated to dysplasia/cancer; methylation ofMSH6, TIMP3was significantly associated to IBD-related dysplasia/cancer. Promoter methylation ofMSH6, MSH3, RUNX3, CRABP1, TP73, RARB, CDH13, PAX5, WT1, THBS1, TP53, SFRP1, WIF1, APAF1,BCL2genes was significantly associated to active IBD. Conclusions:Methylation analysis, namely ofMSH6, may contribute to the classification of dysplastic lesions in IBD- to be further tested in prospective studies.
dc.description.sponsorshipGrupo de Estudos em Doenca Inflamatoria Intestinal (GEDII), Portugal, a non-profit Portuguese research organization
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1080/00365521.2020.1766552
dc.identifier.issn0036-5521
dc.identifier.urihttp://hdl.handle.net/10400.1/16479
dc.language.isoeng
dc.peerreviewedyes
dc.publisherTaylor and Francis
dc.subjectInflammatory bowel disease
dc.subjectDysplasia
dc.subjectCancer
dc.subject.otherGastroenterology & hepatology
dc.titleMethylation patterns in dysplasia in inflammatory bowel disease patients
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage655
oaire.citation.issue6
oaire.citation.startPage646
oaire.citation.titleScandinavian Journal of Gastroenterology
oaire.citation.volume55
person.familyNameSousa
person.familyNameRoseira
person.givenNameHelena Tavares
person.givenNameJoana
person.identifier.ciencia-idCF1F-1163-1C4A
person.identifier.orcid0000-0002-6626-205X
person.identifier.orcid0000-0002-5098-8729
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication6b1d11dd-486f-4fb3-b41f-02e1cf6a5c2e
relation.isAuthorOfPublication077331f1-402e-455f-8e16-e475d62bc73a
relation.isAuthorOfPublication.latestForDiscovery6b1d11dd-486f-4fb3-b41f-02e1cf6a5c2e

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