Percorrer por autor "Ślusarczyk, Sylwester"
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- Dataset on functional and chemical properties of the medicinal halophyte Polygonum maritimum L. under greenhouse cultivationPublication . Rodrigues, Maria Joao; Monteiro, Ivo; Placines, Chloé; Castañeda-Loaiza, Viana; Ślusarczyk, Sylwester; Matkowski, Adam; Pereira, Catarina; Pousão-Ferreira, Pedro; Custódio, LuísaThis data article includes data and analyses on the effect of different agronomic techniques on the production of Polygonum maritimum L. (sea knotgrass), namely different salinity irrigation treatments (0, 100, 200, 300 and 600 mM of NaCl) and a multi-harvest regime, and their relation with the chemical profile (ultra-high-resolution mass spectrometry - UHRMS), in vitro antioxidant [radical-scavenging activity (RSA) of DPPH and ABTS, copper chelating activity and ferric reducing antioxidant power] and anti-inflammatory (nitric oxide reduction on lipopolysaccharide-stimulated macrophages) activities. For further interpretation of the data presented in this work, please see the related research article "The irrigation salinity and harvesting affect the growth, chemical profile and biological activities of Polygonum maritimum L." (Rodrigues et al., 2019).
- Greener is better: first approach for the use of natural deep eutectic solvents (NADES) to extract antioxidants from the medicinal halophyte Ppolygonum maritimum L.Publication . Rukavina, Iva; Rodrigues, Maria João; Pereira, Catarina; Mansinhos, Inês; Romano, Anabela; Ślusarczyk, Sylwester; Matkowski, Adam; Custódio, LuísaIn this study, natural deep eutectic solvents (NADES) formed by choline chloride (ChCl), su crose, fructose, glucose, and xylose, were used to extract antioxidants from the halophyte Polygonum maritimum L. (sea knotgrass) and compared with conventional solvents (ethanol and acetone). NADES and conventional extracts were made by an ultrasound-assisted procedure and evaluated for in vitro antioxidant properties by the radical scavenging activity (RSA) on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, oxygen radical absorbance capacity (ORAC), and cop per chelating activity (CCA). Samples were profiled by liquid chromatography (LC)-electrospray ionization (ESI)-QTOF-MS analysis. ChCl:fructose was more efficient in the DPPH assay, than the acetone extract. ChCl:sucrose and ChCl:fructose extracts had the highest ORAC when compared with the acetone extract. NADES extracts had higher CCA, than the acetone extract. The phenolic composition of the NADES extracts was less complex than the conventional extracts, but the pro portions of major antioxidants, such as flavonols and flavan-3-ols, were similar in all the solvents. Myricitrin was the major flavonoid in all of the samples, while gallic acid was the main phenolic acid in the conventional extracts and present in a greater amount in ChCl:fructose. Results suggest that NADES containing ChCl and sucrose/fructose can replace conventional solvents, especially acetone, in the extraction of antioxidants from sea knotgrass.
- Salicylic acid induces dose-dependent and organ-specific modulation of phenolic profiles and antioxidant activity in Polygonum maritimum L. in vitro culturesPublication . Oyesiji, Adeola; Ślusarczyk, Sylwester; Matkowski, Adam; Fernandes, Eliana; Binnert, Marie; Braga, Tiago; Custódio, Luísa; Rodrigues, Maria JoãoPolygonum maritimum L. (sea knotgrass) is a salt-tolerant medicinal plant rich in phenolic compounds with recognised antioxidant potential and growing interest for pharmaceutical and nutraceutical applications. This study evaluated the effects of salicylic acid (SA) elicitation on growth, phenolic metabolite profiles, and antioxidant activity in roots and shoots of P. maritimum in vitro cultures. Shoots were cultured for four weeks on control medium or medium supplemented with 50, 100, or 200 µM SA. The roots formed during the treatment were separated from the shoots and analysed independently. Phenolic profiles of both organs were characterised by LC–ESI–QTOF–MS, and antioxidant activity was assessed using DPPH, ABTS, FRAP, and copper-chelating activity assays. SA elicitation induced clear dose-dependent responses, with increasing concentrations reducing biomass and altering metabolite composition. Roots exhibited substantially greater chemical diversity than shoots, including catechin-type flavonoids, quercetin derivatives, and phenolic acids, whereas shoots displayed a simpler profile comprising a gallic acid derivative and flavonoid glycosides. In roots, 50 and 100 µM SA were associated with broader metabolite profiles, whereas several compounds were no longer detected at 200 µM, suggesting that lower and intermediate SA concentrations may be more suitable for preserving a broader metabolite profile. Antioxidant responses were assay- and organ-dependent, with roots generally displaying stronger radical-scavenging activity, although no single SA concentration consistently maximised bioactivity across all assays. Overall, SA altered biomass, metabolite occurrence, and antioxidant activity in an organ- and concentration-dependent manner. These findings provide a basis for optimising SA elicitation to produce P. maritimum biomass with tailored phenolic profiles for potential nutraceutical, pharmaceutical, and cosmetic applications.