Browsing by Issue Date, starting with "2019"
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- Identification and validation of microsatellite markers in strawberry tree (Arbutusunedo L.)Publication . Fazenda, Pedro; Pereira, Ricardo; Fonseca, Maria; Carlier, Jorge; Leitão, JoséStrawberry tree (Arbutus unedo L.), an evergreen shrub/small tree of the family Ericaceae, is a main constituent of the Mediterranean basin flora; although it is also found in southwestern Prance, Macaronesia, and Ireland. The small fruits are edible but mostly used for preparation of preserves and jams, and for liquors such as the Portuguese traditional "aguardente de medronho". Traditionally cultivated by small farmers, often in consociation with Quercus sp., strawberry tree is presently emerging as a new important fruit crop cultivated in large orchards by modern export-oriented enterprises. This change of paradigm requires a growing role of plant breeding, upstream of the production process. Genomic tools for this species are mostly limited to the chloroplast genome sequence and to genomic data described in this work. In order to identify strawberry tree microsatellite (SSR) loci we performed partial genome next-generation sequencing using the Ion Torrent technology. The sequenced similar to 24.6M nucleotides resulted in the identification of 1185 microsatellite markers mostly constituted by dinucleotide motifs. The relative amount of microsatellite dinucleotide motifs (AG/CT - 71.7%, AC/GT - 20.5%, AT/AT - 2.9%, and CG/CG - 0.3%) is similar to the one observed in other Ericaceae species. Among a tested sample of 40 SSR primer pairs, 20 amplified well-defined PCR products, 12 (30%) were validated as polymorphic. Used in our collaborative project for molecular identification of selected and improved clones, the identified SSR loci constitute a strong tool for a large panoply of applied and fundamental studies of this emerging fruit crop.
- NILM techniques for intelligent home energy management and ambient assisted living: a reviewPublication . Ruano, Antonio; Hernandez, Alvaro; Ureña, Jesus; Ruano, Maria; Garcia, JuanThe ongoing deployment of smart meters and different commercial devices has made electricity disaggregation feasible in buildings and households, based on a single measure of the current and, sometimes, of the voltage. Energy disaggregation is intended to separate the total power consumption into specific appliance loads, which can be achieved by applying Non-Intrusive Load Monitoring (NILM) techniques with a minimum invasion of privacy. NILM techniques are becoming more and more widespread in recent years, as a consequence of the interest companies and consumers have in efficient energy consumption and management. This work presents a detailed review of NILM methods, focusing particularly on recent proposals and their applications, particularly in the areas of Home Energy Management Systems (HEMS) and Ambient Assisted Living (AAL), where the ability to determine the on/off status of certain devices can provide key information for making further decisions. As well as complementing previous reviews on the NILM field and providing a discussion of the applications of NILM in HEMS and AAL, this paper provides guidelines for future research in these topics.
- Increased intestinal carbonate precipitate abundance in the sea bream (Sparus aurata L.) in response to ocean acidificationPublication . Gregorio, Silvia; Ruiz-Jarabo, Ignacio; Carvalho, Edison S. M.; Fuentes, JuanMarine fish contribute to the carbon cycle by producing mineralized intestinal precipitates generated as by-products of their osmoregulation. Here we aimed at characterizing the control of epithelial bicarbonate secretion and intestinal precipitate presence in the gilthead sea bream in response to predicted near future increases of environmental CO2. Our results demonstrate that hypercapnia (950 and 1800 μatm CO2) elicits higher intestine epithelial HCO3- secretion ex vivo and a subsequent parallel increase of intestinal precipitate presence in vivo when compared to present values (440 μatm CO2). Intestinal gene expression analysis in response to environmental hypercapnia revealed the up-regulation of transporters involved in the intestinal bicarbonate secretion cascade such as the basolateral sodium bicarbonate co-transporter slc4a4, and the apical anion transporters slc26a3 and slc26a6 of sea bream. In addition, other genes involved in intestinal ion uptake linked to water absorption such as the apical nkcc2 and aquaporin 1b expression, indicating that hypercapnia influences different levels of intestinal physiology. Taken together the current results are consistent with an intestinal physiological response leading to higher bicarbonate secretion in the intestine of the sea bream paralleled by increased luminal carbonate precipitate abundance and the main related transporters in response to ocean acidification.
- Relationship between substrate, physico-chemical parameters and foraminiferal tests in the Doñana National Park, a Biosphere Reserve in SW SpainPublication . Guerra, Liliana; C. Veiga-Pires, C.; González Regalado, María Luz; Abad, Manuel; Toscano, Antonio; Muñoz, Juan Manuel; Ruiz, Francisco; Rodríguez Vidal, Joaquín; Cáceres, Luis Miguel; Izquierdo, Tatiana; Carretero, María Isabel; Pozo, Manuel; Monge, Guadalupe; Tosquella, Josep; Prudencio, María Isabel; Dias, María Isabel; Marques, Rosa; Gómez, Paula; Romero, VerónicaA multidisciplinar analysis of sediments collected in diferent environments of the Doñana National Park (Guadalquivir estuary, SW Spain) provides an overview of the textural, mineralogical and physico-chemical parameters that control the distribution of benthic foraminiferal tests in this Biosphere Reserve. These microorganisms are absent in the fne quartzitic sands that constitute the substrate of temporary ponds with brief hydroperiods located in the dune systems and spits, as well as in other ponds with low conductivities or hypersaline conditions located in the inner marshlands or near the Guadalquivir river banks. Dead benthic foraminifera are mainly found on phyllosilicate-rich, silty-clayey substrates. The taphonomic analysis of the main species (Ammonia tepida, Haynesina germanica, Trochammina infata, Entzia macrescens) points to its deposit in situ. Cluster analysis permits to delimitate six foraminiferal assemblages. Cluster II (A. tepida+H. germanica) is the dominant assemblage in the central ponds and the margins of the main channels, while cluster IV (T. infata+E. macrescens) is restricted to some ponds located on the high marsh and cluster VI (Ammonia beccarii+Quinqueloculina spp.) is abundant on external beaches. Tidal fuxes cause the transport of these last marine benthic species and some plaktonic forms both to the inner areas of the estuary and to these beaches.
- Digital marketing actions that achieve a better attraction and loyalty of users: an analytical studyPublication . Ramos, Celia; Matos, Nelson; López García, Juan José; Lizcano, DavidCurrently, the digital economy contributes decisively to an increase in competitiveness, especially as a digital transformation involves migrating to new technological models where digital marketing is a key part of growth and user loyalty strategies. Internet and Digital Marketing have become important factors in campaigns, which attract and retain Internet users. This study aims to identify the main ways in which users can be gained and retained by using Digital Marketing. The Delphi method with in-depth interviews was the methodology used in this study. The results of the research show the most important actions for achieving user recruitment and loyalty with Digital Marketing from the opinions of consulted experts. The limitations of this study are those related to the number of experts included in the study, and the number of research papers consulted in the literature review. The literature review and the results of this research are used to propose new solid research with a consolidated critical methodology. This research deals with a new approach that will optimize web technologies for the evolution of user trends, and therefore, will be of academic and professional use for marketing managers and web solution developers. The conclusions of the investigation show the key factors, discarding others that do not affect the optimization of conversions in B2C businesses such as the duration of the session and the rebound percentage. Likewise, the results of the research identify the specific actions that must be carried out to attract and retain users in B2C companies that use the Digital Marketing ecosystem on the Internet. The requirements for companies that wish to implement a model to optimize conversions using the current digital economy are also shown.
- Biogeographical patterns of endolithic infestation in an invasive and an indigenous intertidal marine ecosystem engineerPublication . Ndhlovu, Aldwin; McQuaid, Christopher D.; Nicastro, Katy; Marquet, Nathalie; Gektidis, Marcos; Monaco, Cristián J.; Zardi, GerardoBy altering the phenotypic properties of their hosts, endolithic parasites can modulate the engineering processes of marine ecosystem engineers. Here, we assessed the biogeographical patterns of species assemblages, prevalence and impact of endolithic parasitism in two mussel species that act as important ecosystem engineers in the southern African intertidal habitat, Perna perna and Mytilus galloprovincialis. We conducted large-scale surveys across three biogeographic regions along the South African coast: the subtropical east coast, dominated by the indigenous mussel, P. perna, the warm temperate south coast, where this species coexists with the invasive Mediterranean mussel, M. galloprovincialis, and the cool temperate west coast dominated by M. galloprovincialis. Infestation increased with mussel size, and in the case of M. galloprovincialis we found a significantly higher infestation in the cool temperate bioregion than the warm temperate region. For P. perna, the prevalence of infestation was higher on the warm temperate than the subtropical region, though the difference was marginally non-significant. On the south coast, there was no significant difference in infestation prevalence between species. Endolith-induced mortality rates through shell collapse mirrored the patterns for prevalence. For P. perna, endolith species assemblages revealed clear grouping by bioregions. Our findings indicate that biogeography affects cyanobacteria species composition, but differences between biogeographic regions in their effects are driven by environmental conditions.
- Surface-ocean dynamics during eccentricity minima: a comparison between interglacial Marine Isotope Stage (MIS) 1 and MIS 11 on the Iberian MarginPublication . Palumbo, Eliana; Voelker, Antje; Flores, Jose Abel; Amore, Ornella F.Understanding interglacial climate variability is a key issue in the scientific community. Here we compared records from Marine Isotope Stage (MIS) 11 to those from MIS 1 (Holocene) as they are perceived to be possible analogs. Our study on the Iberian Margin, a key area to investigate surface dynamics in the Atlantic Ocean, incorporates coccolithophore assemblage and alkenone data of core MD03-2699 and their statistical analyses. Evaluating similarities between MIS 11 and MIS 1 depends on the way the two MIS are being aligned, i.e. at the deglaciation or based on the precession signal. During the deglaciation of either MIS 12 or MIS 2, the Iberian Margin was affected by abrupt decreases in SST and in coccolithophores' paleoproductivity caused by the arrival of subpolar surface waters. Just prior to the decline, in both the intervals, the Portugal Current affected the studied site, although a possible difference in upwelling strength is here suggested and related to more intense westerlies during the last glacial than the late MIS 12. Similar surface-ocean dynamics occurred at the onset of both MIS 11 and MIS 1 as indicated by the prevalence of the Iberian Poleward Current and sometimes the Azores Current, although the subtropical waters were more oligotrophic during the MIS 2 deglaciation than the MIS 12 one. Synchronizing our records according to the precession cycles aligns the early-to-mid Holocene with the second, warmer phase of MIS 11c. During both these intervals, the western Iberian Margin was mainly affected by the Iberian Poleward Current that transported more temperate-warm, mesotrophic surface waters during MIS 11c than during the early-to-mid Holocene. During the early to mid-Holocene the Iberian Margin endured incursions of colder surface waters that did not occur during MIS 11c allowing us to hypothesize that the studied site experienced, from a paleoceanographic point of view, a more stable period during MIS 11c than the early Holocene. Finally, spectral analysis suggests the role of full, half and fourth precession components in driving surface-ocean variability during MIS 11 and during the last 24 kyr BP.
- O que é um bom médico?Publication . Ponte, JoséDesde a entrada numa faculdade, formar um especialista em medicina leva normalmente entre 10 e 12 anos, dependendo da especialidade. Seis anos para o curso básico, e entre quatro a seis anos para o internato médico. Sabe-se que é difícil definir objetivamente o que é ‘qualidade’ num médico recém-formado ou num especialista que terminou o seu internato, mas é largamente aceite, infelizmente, que as notas obtidas nos exames reflectem a qualidade. O consenso atual, adotado por instituições reguladoras da educação médica em vários países1–3 define, por ordem de importância, três vertentes fundamentais (outcomes) da educação médica, aplicáveis a qualquer nível da formação: valores e comportamento profissionais, competências clínicas práticas, incluindo comunicação, e conhecimentos teóricos.
- Fine‐scale genetic structure and flowering output of the seagrass Enhalus acoroides undergoing disturbancePublication . Yu, Shuo; Wu, Yunchao; Serrao, Ester A.; Zhang, Jingping; Jiang, Zhijian; Huang, Chi; Cui, Lijun; Thorhaug, Anitra; Huang, XiaopingSeagrass are under great stress in the tropical coast of Asia, where Enhalus acoroides is frequently the dominant species with a large food web. Here, we investigate the question of the fine-scale genetic structure of this ecologically important foundation species, subject to severe anthropogenic disturbance in China. The genetic structure will illuminate potential mechanisms for population dynamics and sustainability, which are critical for preservation of biodiversity and for decision-making in management and restoration. We evaluated the fine-scale spatial genetic structure (SGS) and flowering output of E. acoroides, and indirectly estimated the relative importance of sexual versus asexual reproduction for population persistence using spatial autocorrelation analysis. Results reveal high clonal diversity for this species, as predicted from its high sexual reproduction output. The stronger Sp statistic at the ramet-level compared with genet-level indicates that clonality increases the SGS pattern for E. acoroides. Significant SGS at the genet-level may be explained by the aggregated dispersal of seed/pollen cohorts. The estimated gene dispersal variance suggests that dispersal mediated by sexual reproduction is more important than clonal growth in this study area. The ongoing anthropogenic disturbance will negatively affect the mating pattern and the SGS patterns in the future due to massive death of shoots, and less frequency of sexual reproduction.
- Editorial: active ageing, social inclusion and wellbeing: benefits of learning in later lifePublication . Schmidt-Hertha, Bernhard; Formosa, Marvin; Almeida, António Fragoso deThe education of older adults has been considered the fastest growing branch of adult education in post-industrial countries and one of the most crucial challenges facing current adult European education (Formosa, 2000). Early research on the learning preferences, motivations and trends of older persons – as well as the impact of learning on the quality of life of older learners – can be traced to the 1950s (Havighurst, 1953), even before the field of educational gerontology was formally established in the 1975 by David Peterson (1976). In recent years, an unprecedented level of influence of the concept of lifelong learning on policies on active ageing have led to a ‘renaissance’ moment in the practice and research of older adult learning (Glendenning 1992; Findsen & Formosa, 2016). Whilst at the turn of the millennium, one found only a handful of book publications in the field of older adult learning, and the few published articles were often in specialised and off the radar journals, in a space of less than two decades the situation is markedly different. Nowadays, as societies are experiencing, or anticipating, unprecedented number of older persons, the field of late-life learning is firmly established in both adult education and gerontology graduate programmes, as well as mainstream adult education and gerontology journals. Indeed, the field of older adult learning boasts an exciting and innovative field of practice, led by experts who group themselves under the mantles of adult educators, educational gerontologists, geragogists or gerontagogists (Kern, 2014). Learning in later life has entrenched itself as an integral part of adult education research, focusing on the diverse provision of late-life learning, the motivations and interests of older learners; wide-participation and emancipatory policies for older adult learning; and the benefits of learning for learners, providers, and society in general.