Browsing by Author "Almansa, E."
Now showing 1 - 5 of 5
Results Per Page
Sort Options
- A general survey of the feasibility of culturing the mysid Gastrosaccus roscoffensis (Peracarida, Mysida): growth, survival, predatory skills, and lipid compositionPublication . Escanez, A.; Riere, R.; Marquez, L.; Skalli, A.; Felipe, B. C.; Garcia-Herrero, I.; Reis, D.; Rodriguez, C.; Almansa, E.The effects of culture conditions on growth, survival, predation, and nutritional composition of the mysid Gastrosaccus roscoffensis were studied. Light had a negative effect on the predation rates of G roscoffensis and predator size was important to prey on Artemia nauplii and rotifers (Brachionus plicatilis). Growth rates were higher in the first three weeks and measured individuals increased almost 7 mm in length in six weeks. Mortality rate was not constant, but it peaked during the first 10 days and after the 40th day of culture. Lipid class composition of cultured G. roscoffensis juveniles showed differences compared to wild G roscoffensis and other mysid species, with a high proportion of neutral lipids (72.04% total lipids) mainly triacylglycerol (41.74%). Fatty acid composition was characterized by high levels of 18:3n-3 (23.16% total lipids) and monoenes in cultured G. roscoffensis. The experiments demonstrated the feasibility of the culturing conditions assayed for G roscoffensis, although further experiments should be carried out to test this mysid as a prey for new species of commercial interest, including fish and cephalopods.
- An insight on Octopus vulgaris paralarvae lipid requirements under rearing conditionsPublication . Reis, Diana; García-Herrero, I.; Riera, R.; Felipe, B. C.; Rodríguez, C.; Sykes, António V.; Martín, M. V.; Andrade, José Pedro; Almansa, E.In this study, two new alternative preys: Grapsus adscensionis zoeae (as sole prey) and Palaemon elegans zoeae (in cofeeding with Artemia sp.), as well as, Artemia sp. juveniles were used as feed for octopus paralarvae, as a way to understand its lipid requirements. Total lipid (TL) content, lipid class (LC) and fatty acid (FA) profiles of preys, octopus hatchlings and 9-day-old paralarvae were analysed. Growth and survival of the paralarvae were also determined. Regardless the prey provided, a notable shift in the lipid profile of paralarvae was registered after 9 days of rearing. The highest index of growth rate (IGR) recorded when decapod crustacean zoeae were supplied might have some relation with levels of 20:4n-6 (ARA) and DHA/EPA ratio observed. In this sense, Grapsus adscensionis zoeae leaded to a higher content of ARA and a lower content of EPA, which may indicate a possible competition between these two FA. For that a balanced EPA/ARA ratio might be significant in this species nutrition without disconsidering DHA levels as an essential fatty acid.
- Effects of culture density and live prey on growth and survival of juvenile cuttlefish, Sepia officinalisPublication . Domingues, P.; Poirier, R.; Dickel, L.; Almansa, E.; Sykes, António V.; Andrade, José PedroThe European cuttlefish, Sepia offıcinalis Linnaeus 1758, has a number of characteristics, such as a fast growth rate and benthic life style, which make this one of the most promising species for large-scale culture (Domingues 1999). One of the key factors in large-scale aquaculture is to optimise growth while minimizing the culture space required (Forsythe et al. 2002). Until now, only Nabhitabhata (1999) and Forsythe et al. (1994), Koueta and Boucaud-Camou (1999) indicated stocking densities for cultured cuttlefish populations. Laboratory-cultured cuttlefish have been studied in several aspects of their behaviour. Nevertheless, effects of tank size and animal density on growth, condition and reproduction are not known (Boal et al. 1999). Crowding can have a major effect on growth and behaviour in many species, and in aquaculture this factor can be very important (Sumpter 1993).
- In vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexesPublication . Reis, Diana; Acosta, N. G.; Almansa, E.; Navarro, J. C.; Tocher, D. R.; Monroig, O.; Andrade, José Pedro; Sykes, António V.; Rodríguez, C.The highmortalities observed during Octopus vulgaris paralarvae culture have been associated with a nutritional imbalance, with long-chain polyunsaturated fatty acids (LC-PUFA) appearing to have a critical role. In order to determine the in vivo capability of O. vulgaris hatchlings to incorporate and metabolise unsaturated fatty acids (FA), hatchlings were incubated in flat-bottom 6-well tissue culture plates at a density of 90 hatchlings/well in 10 mL of seawater (36‰). Incubations were performed with gentle stirring at 21 °C for 6 h with 0.2 μCi (0.3 μM) of [1−14C]-labelled FA including 18:1n−9, 18:2n−6, 18:3n−3, 20:4n−6 (ARA), 20:5n−3 (EPA) or 22:6n−3 (DHA), which were added directly to the seawater as their potassium salts bound to bovine serum albumin (BSA). A control treatmentwithout [1−14C]FA was also assessed. O. vulgaris hatchlings not only possessed the ability to incorporate FA bound to BSA, but also to esterify them into phospholipid, with marked specificity. [1−14C]DHA and [1−14C]C18 FA substratesweremainly esterified into phosphatidylcholine, while [1−14C]ARA and [1−14C]EPA were esterified into phosphatidylethanolamine.
- In vivo metabolism of unsaturated fatty acids in Sepia officinalis hatchlingsPublication . Reis, Diana; Rodriguez, C.; Acosta, N. G.; Almansa, E.; Tocher, D. R.; Andrade, José Pedro; Sykes, António V.The transition of Sepia officinalis culture to industrial large scale has been hampered due to bottlenecks related to the limited knowledge on nutritional physiology of the species. Determination of the endogenous ability of S. officinalis hatchlings to metabolise unsaturated fatty acids (FA) may provide new insight on the capability of hatchlings to biosynthesise different FA, as well as lipid classes containing essential fatty acids (EFA). In the present study, cuttlefish hatchlings were incubated with [1-C-14] FA including C18 FA (18:1n-9, 18:2n-6, 18:3n-3) and long-chain polyunsaturated fatty acids (LC-PUFA) (20: 4n-6 (ARA), 20:5n-3 (EPA) or 22:6n-3 (DHA)), which were added individually as potassium salts bound to bovine serumalbumin. As a result, it was possible to investigate the in vivo FAmetabolismof S. officinalis hatchlings by following the incorporation of specific [1-C-14] FA, which points to the suitability of this methodology to study lipid metabolism of newly hatched cephalopods. The majority of radioactivity incorporated was recovered esterified into polar lipids (PL). A pattern was detected, where [1-C-14] DHA, [1-C-14] C18 FA and their metabolic products were preferentially esterified into phosphatidylcholine, whereas [1-C-14] ARA and [1-C-14] EPA were mainly esterified into phosphatidylethanolamine. [1-C-14] C18 FA were the most transformed FA with several metabolites produced by elongation and possible desaturation being obtained. As a contrary the radioactivity incorporated into hatchling total lipid (TL) from supplemented [1-C-14] LC-PUFA only one elongation product was recovered from the three substrates, except for [1-C-14] ARA, where an unidentified product was also detected. The present in vivo results indicated that S. officinalis hatchlings may have capability for the first steps in the biosynthesis of ARA and EPA from 18: 2n-6 and 18: 3n-3, respectively, including the existence of a desaturase potentially involved. Nonetheless, considering the low desaturation rates detected, this process may not be sufficient to cover EFA demands during development of this species. Therefore, dietary ARA and EPA, as well as DHA, should be supplied during the hatchling stage of Sepia. While designing an inert diet, which ensures normal growth and development of this species during the hatchling stage, the C18 FA and LC-PUFA levels and ratios should be considered, since the esterification pattern detected in the present study suggested competition between these FA for esterification into specific lipid classes. Moreover, considering the observed esterification pattern of LC-PUFA into different lipid classes, it is likely that the DHA/EPA/ARA ratio, rather than DHA/EPA or EPA/ARA ratios, would be of great importance for S. officinalis hatchling development. (C) 2015 Elsevier B.V. All rights reserved.