Browsing by Author "Azevedo, Jorge"
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- Single strand conformation polymorphism (SSCP) detection in six genes in the Portuguese indigenous sheep breed "Churra da Terra Quente"Publication . Bastos, Estela; Cravador, A.; Azevedo, Jorge; Guedes-Pinto, HenriqueEvaluation of the genetic diversity for six genes in forty animals of the Portuguese indigenous sheep breed (Ovis aries) “Churra da Terra Quente” was done. A non-radioactive method to allow single-strand conformation polymorphism (SSCP) detection was optimised, starting from genomic DNAand PCR amplification of seven fragments: exon 1 of the a-lactalbumin gene; exons 10 and 11 of the as1-casein gene; exon 7 of the b-casein gene; exon 4 of the k-casein gene; exons 4 and 5 of the growth hormone gene and exon 6 of the growth hormone receptor gene. Polymorphisms were detected in five of the PCR products. Only k-casein and growth hormone receptor were monomorphic. a-lactalbumin and as1-casein exons showed three conformational patterns, b-casein and growth hormone exon 4 showed two electrophoretic patterns and growth hormone exon 5 showed five conformational patterns. These data provide evidence that “Churra da Terra Quente” has a high genetic variability, which opens interesting prospects for future selection programs and also for preservation strategies. Also, our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability.
- Single-strand conformation polymorphism (SSCP) analysis of exon 4 and exon 5 of growth hormone gene in “Serrana Transmontana” goat breedPublication . Varejão, M. C.; Bastos, Estela; Chaves, R.; Cravador, A.; Azevedo, Jorge; Guedes-Pinto, HenriqueThe origin of Serrana goat breed is lost in time: however the wild goat Capra pyrenaica, from the Serra da Estrela, is probably its ancestor. The Serrana is the most representative of Portuguese breeds and is considered to have great productive potentialities and considerable expansion possibilities due to its productive and reproductive indices, high degree of ruggedness and high quality products: cheese and meat.