Percorrer por autor "Cancio, I."
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- Accumulation and toxicity of copper oxide nanoparticles in the digestive gland of Mytilus galloprovincialisPublication . Gomes, Tânia; Pereira, Catarina Guerreiro; Cardoso, Cátia; Pinheiro, José Paulo; Cancio, I.; Bebianno, Maria JoãoGiven the wide use of CuO nanoparticles in various industrial and commercial applications they will inevitably end up in the aquatic environment. However, little information exists on their biological effects in bivalve species. Accordingly, mussels Mytilus galloprovincialis were exposed to 10 g Cu L−1 as CuO nanoparticles and Cu2+ for 15 days, and biomarkers of oxidative stress (superoxide dismutase, catalase and glutathione peroxidase), damage (lipid peroxidation) and metal exposure (metallothionein) were determined along with Cu accumulation in the digestive glands of mussels. Cu was linearly accumulated with time of exposure in mussels exposed to CuO nanoparticles, while in those exposed to Cu2+ elimination was significant by day 15. Both forms of Cu cause oxidative stress with distinct modes of action. Exposure to CuO nanoparticles induces lower SOD activity in digestive glands compared to those exposed to Cu2+, while CAT was only activated after 7 days of exposure to nano and ionic Cu, with contradictory effects after 15 days of exposure and GPX activities were similar. Lipid peroxidation levels increased in both Cu forms despite different antioxidant efficiency. Moreover, a linear induction of metallothionein was detected with time in mussels exposed to CuO nanoparticles, directly related to Cu accumulation, whereas in those exposed to Cu2+ metallothionein was only induced after 15 days of exposure. Since only a small fraction of soluble Cu fraction was released from CuO nanoparticles, the observed effects seem to be related to the nano form of Cu, with aggregation as a key factor. Overall, our results show that the digestive gland is susceptible to CuO nanoparticles related oxidative stress, and is also the main tissue for their accumulation.
- Effects of copper nanoparticles exposure in the mussel Mytilus galloprovincialisPublication . Gomes, Tânia; Pinheiro, J. P.; Cancio, I.; Pereira, Catarina Guerreiro; Cardoso, Cátia; Bebianno, Maria JoãoCuO NPs are widely used in various industrial and commercial applications. However, little is known about their potential toxicity or fate in the environment. In this study the effects of copper nanoparticles were investigated in the gills of mussels Mytilus galloprovincialis, comparative to Cu(2+). Mussels were exposed to 10 μg Cu·L(-1) of CuO NPs and Cu(2+) for 15 days, and biomarkers of oxidative stress, metal exposure and neurotoxicity evaluated. Results show that mussels accumulated copper in gills and responded differently to CuO NPs and Cu(2+), suggesting distinct modes of action. CuO NPs induced oxidative stress in mussels by overwhelming gills antioxidant defense system, while for Cu(2+) enzymatic activities remained unchanged or increased. CuO NPs and Cu(2+) originated lipid peroxidation in mussels despite different antioxidant efficiency. Moreover, an induction of MT was detected throughout the exposure in mussels exposed to nano and ionic Cu, more evident in CuO NPs exposure. Neurotoxic effects reflected as AChE inhibition were only detected at the end of the exposure period for both forms of copper. In overall, these findings show that filter-feeding organisms are significant targets for nanoparticle exposure and need to be included when evaluating the overall toxicological impact of nanoparticles in the aquatic environment.
- The ocean sampling day consortiumPublication . Kopf, Anna; Bicak, Mesude; Kottmann, Renzo; Schnetzer, Julia; Kostadinov, Ivaylo; Lehmann, Katja; Fernandez-Guerra, Antonio; Jeanthon, Christian; Rahav, Eyal; Ullrich, Matthias; Wichels, Antje; Jones, Scott; Orlic, Sandi; Steinke, Michael; Busch, Julia; Duarte, Bernardo; Caçador, Isabel; ten Hoopen, Petra; Canning-Clode, João; Aguirre-Macedo, Ma L.; Bobrova, Oleksandra; Vezzi, Alessandro; Marteinsson, Viggo; Collin, Rachel; Reynisson, Eyjolfur; Loureiro, Clara M.; Luna, Gian M.; Quero, Grazia M.; Löscher, Carolin R.; Kremp, Anke; Amaral, Valentina; DeLorenzo, Marie E.; Yoshida, Takashi; Øvreås, Lise; Wang, Shiao; Fuhrman, Jed A.; Tolman, Jennifer; LaRoche, Julie; Penna, Antonella; Frischer, Marc; Davis, Timothy; Katherine, Barker; Meyer, Christopher P.; Ogata, Hiroyuki; Conan, Pascal; Todorova, Nadezhda; Alonso, Cecilia; Stambler, Noga; Goodwin, Kelly; Nyhus, Paul A. F.; Yakimov, Michael M.; Santana, Rafael; Baltar, Federico; Bodrossy, Levente; Ingleton, Tim; Van De Kamp, Jodie; Frampton, Dion M.; Ostrowski, Martin; Van Ruth, Paul; Karamfilov, Ventzislav; Malthouse, Paul; Bizsel, Kemal C.; Claus, Simon; Deneudt, Klaas; Pedrotti, Maria L.; Munnik, Kate; Mortelmans, Jonas; Pitois, Sophie; Wallom, David; Salter, Ian; Costa, Rodrigo; Schroeder, Declan C.; Kandil, Mahrous M.; Rodriguez-Ezpeleta, Naiara; Kotoulas, Georgios; Berteaux-Lecellier, Veronique; Cochrane, Guy; Wecker, Patricia; Cariou, Thierry; Cancio, I.; Lauro, Federico M.; Vaulot, Daniel; Bienhold, Christina; Ghazal, Hassan; Chaouni, Bouchra; Essayeh, Soumya; Ettamimi, Sara; Iriberri, Juan; Zaid, El H.; Golyshin, Peter N.; Boukhatem, Noureddine; L’Haridon, Stephane; Martin, Patrick; Bouali, Abderrahim; Chahboune, Rajaa; Barrijal, Said; Timinouni, Mohammed; El Otmani, Fatima; Bennani, Mohamed; Mea, Marianna; Gasol, Josep M.; Jensen, Rachelle M.; Gerdts, Gunnar; Hinks, Jamie; Gebbels, Susan; Rosselli, Riccardo; Jude-Lemeilleur, Florence; De Pascale, Fabio; Bente, Edvardsen; Schiavon, Riccardo; dos Santos, Antonina; Moncheva, Snejana; Villar, Emilie; Pesant, Stéphane; Cataletto, Bruno; Malfatti, Francesca; Polymenakou, Paraskevi; Edirisinghe, Ranjith; Sonnenschein, Eva C.; Silveira, Jorge A. H.; Barbier, Michele; Karlsen, Hans E.; Dzhembekova, Nina; Turk, Valentina; Tinta, Tinkara; Fuller, Wayne J.; Salihoglu, Ilkay; Serakinci, Nedime; Ergoren, Mahmut C.; Bresnan, Eileen; Johnson, Zackary; Ramos, Sandra; Sinigalliano, Christopher D.; Siam, Rania; Gidley, Maribeth L.; Biancalana, Florencia; Zingone, Adriana; O’Gara, Fergal; Danovaro, Roberto; Tsiamis, George; Clark, M. S.; Costa, Ana C.; El Bour, Monia; Martins, Ana M.; Magalhães, Catarina; Collins, R. E.; Poulton, Nicole; Ducluzeau, Anne-Lise; Abdallah, Rehab Z.; Jackson, Stephen; Martinez, Jonathan; Costello, Mark J.; Amaral-Zettler, Linda A.; Gilbert, Jack A.; Davies, Neil; Field, Dawn; Glöckner, Frank O.Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world’s oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits.