Percorrer por autor "Costa, Sara"
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- Aprender com os conhecimentos e as experiências das criançasPublication . Coelho, Adriana; Paretskaya, Anna; Tavares, Andreia; Monho, Andreia; Lucas, Cássia; Fernandes, Catarina; Palma, Inês; Gomes, Mafalda; Arvela, Margarida; Pereira, Margarida; Neves, Mariana; Rosário, Marta; Brito, Nadine; Rocha, Rute; Costa, Sara; Pereira, Vera; Gil, Francisco Baptista; Rocha, Rute; Fernandes, CatarinaCerta vez, perguntei a uma criança: "Qual é a forma da Terra?" Ela respondeu: "Na sala de aula, por causa da professora, digo que a Terra é redonda. Mas quando vou de viagem, o carro nunca faz uma curva para cima e para baixo!" Este aparente paradoxo foi o ponto de partida para a elaboração deste livro, intitulado «Aprender com os conhecimentos e as experiências das crianças». A resposta espontânea da criança revela uma realidade com a qual nos deparamos frequentemente: a convivência simultânea de duas conceções distintas no pensamento infantil — uma de natureza escolarizada e outra de base experiencial. A conceção escolarizada surge do que é ensinado formalmente na escola — o conhecimento “correto” do ponto de vista científico-escolar. Já a conceção experiencial está enraizada nas observações e vivências do quotidiano. Assim, uma criança pode afirmar, em contexto escolar, que a Terra é redonda, mas, fora desse ambiente, perceber o mundo como plano, com base na experiência concreta de se deslocar de carro, sem "curvas para cima ou para baixo". Esse tipo de raciocínio evidencia a importância de reconhecer, escutar e compreender o conhecimento que as crianças já possuem, antes mesmo da intervenção educativa. Como futuras professoras e educadoras, defendemos que é essencial antecipar essas ideias prévias e orientar as crianças no processo de (re)construção do conhecimento, de forma significativa e integrada com as suas experiências diárias
- Lipschutz ulcers: uncommon diagnosis of vulvar ulcerationsPublication . Mourinha, Vera; Costa, Sara; Urzal, Cecilia; Guerreiro, FernandoAetiological diagnosis of genital ulcers is still a challenge in clinical practice. The cause is mostly infectious, but it may otherwise be a presentation of a wide variety of pathologies, such as autoimmune (eg, Behçet’s disease), cancer or inflammatory processes. The Lipschütz ulcer is a non-sexually transmitted condition.
- Phylogenomics as baseline for taxonomy description: Amphibiibacter pelophylacis gen. nov., sp. nov., a novel taxon of the family Sphaerotilaceae, class Betaproteobacteria, isolated from the skin microbiota of Pelophylax perezi from different populationsPublication . Costa, Sara; Proença, Diogo Neves; Lopes, Isabel; Morais, Paula V.Bacterial strain SL12-8T was characterized and isolated from the skin microbiota of Pelophylax perezi, the Perez’s frog. Strain SL12-8T stained Gram-negative and formed rod-shaped cells that grew optimally at 25 °C and pH 7.0–7.5. The G+C content of the DNA was 66.2 mol%. Ubiquinone 8 was the respiratory quinone identified in the studied strain and the most closely related taxon. The major fatty acids were summed in feature 3 and summed in feature 8 and C16:0, representing 84% of the total fatty acids. Phylogenetic analyses based on the 16S rRNA gene sequence placed strain SL12-8T within the order Burkholderiales in a distinct lineage. The 16S rRNA gene sequence similarities of strain SL12-8T to that of Rubrivivax albus, Scleromatobacter humisilvae, Piscinibacter aquaticus, Azohydromonas caseinilytica and Aquincola agrisoli were 94.41, 94.08, 93.72, 93.72 and 93.64%, respectively. The draft genome sequence of strain SL12-8T comprises 3,115,197 bases with a 313-fold mapped genome coverage. The assembled genome consists of 53 large contigs with more than 500 bp, and the genome encodes 2,814 putative coding sequences. The analysis of the available genomes from the closest genera showed 124 core genes that reveal a novel genus-level clade including the strain SL12-8T . Analysis of the SL12-8T genome revealed the presence of the beta-lactone and terpene biosynthetic gene clusters. The phylogenomic, phylogenetic, phenotypic and chemotaxonomic data showed that strain SL12-8T (=UCCCB 131T =CECT 30762T ) represents the type of a novel species and genus, for which we propose the name Amphibiibacter pelophylacis gen. nov., sp. nov.
- Potential protective role of amphibian skin bacteria against water mold saprolegnia spp.Publication . Costa, Sara; Proença, Diogo Neves; Alves, Artur; Morais, Paula V.; Lopes, IsabelAmphibian populations have experienced a severe decline over the past 40 years, driven primarily by environmental pollution, habitat destruction, climate change, and disease. This work reports, for the first time, saprolegniosis in Pelophylax perezi egg masses and saprolegniosis in amphibians in Portugal. After isolation and phylogenetic analysis, the pathogen was identified as Saprolegnia australis. Following this, the present work intended to screen a collection of P. perezi skin bacteria for the existence of bacterial strains with inhibitory action against the newly identified S. australis SC1 and two other species, Saprolegnia diclina SAP 1010 UE and Saprolegnia australis SAP 1581 UE. The results showed that various bacterial species could inhibit the growth of these three species of oomycetes. Bacteria with the most significant antagonistic action against Saprolegnia spp. predominantly belonged to the genus Bacillus, followed by Serratia, Pseudomonas, and Aeromonas. Despite variations in bacterial diversity among frog populations, the present study also demonstrated the presence of bacteria on frogs’ skin that were capable of inhibiting Saprolegnia spp., as evidenced by in vitro challenge assays. These findings highlight the protective function of bacteria present in amphibian skin. The observed bacterial diversity may contribute to the metabolic redundancy of the frog skin microbiome, helping to maintain its functional capacity despite shifts in the community composition. Additionally, the study found that, when providing a more advantageous environment for pathogen growth—in this case a peptone–glucose (PG) medium instead of R2A—the percentage of bacteria with moderate-to-strong antagonistic activity dropped by 13% to 4%. In conclusion, the presence of bacteria capable of inhibiting Saprolegnia spp. in adult individuals and across different environmental conditions may contribute to lowering the susceptibility of frog adults towards Saprolegnia spp., compared with that in the early stages of development, like the tadpole or egg stages.