Browsing by Author "De Vos, M.J."
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- Solid phase non isotopic labelling of oligodeoxynucleotides using 5'-protected aminoalkyl phosphoramidites: Application to the specific detection of human papilloma virus DNAPublication . De Vos, M.J.; Cravador, A.; Lenders, J. P.; Houard, S.; Bollen, A.Phosphoramidites of thymidine or 2'-deoxyinosine, modified in 5' by the addition of an aminoalkylcarbamate function, were prepared. The derivatized nucleotides can be used in automatic DNA synthesis to tag any oligodeoxynucleotide chain and provide a convenient reactive group for labelling with non radioactive reporters. As an example of application, we show the specific detection of Human Papilloma Virus DNA using a biotin-labelled 29-mer oligodeoxynucleotide entirely prepared on solid support.