Solid phase non isotopic labelling of oligodeoxynucleotides using 5'-protected aminoalkyl phosphoramidites: Application to the specific detection of human papilloma virus DNA

De Vos, M.J.; Cravador, A.; Lenders, J. P.; Houard, S.; Bollen, A.

http://hdl.handle.net/10400.1/6139

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
Solid phase.pdf		3.13 MB	Adobe PDF	Download

Send Feedback

Authors

Abstract(s)

Phosphoramidites of thymidine or 2'-deoxyinosine, modified in 5' by the addition of an aminoalkylcarbamate function, were prepared. The derivatized nucleotides can be used in automatic DNA synthesis to tag any oligodeoxynucleotide chain and provide a convenient reactive group for labelling with non radioactive reporters. As an example of application, we show the specific detection of Human Papilloma Virus DNA using a biotin-labelled 29-mer oligodeoxynucleotide entirely prepared on solid support.