Browsing by Author "Defilippi, P."
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- Full-length sequence and expression of the 42 kDa 2-5A synthetase induced by human interferonPublication . Wathelet, M.; Moutschen, S.; Cravador, A.; Dewit, L.; Defilippi, P.; Huez, G.; Content, J.Interferon-induced 2-5A synthetases are probably involved in some antiviral actions of interferon. In human cells two different mRNAs (1.6, 1.8 kb long) coding for this protein are transcribed from the same gene and are produced by differential splicing. The relationship between the two mRNAs of different size and the active enzyme is not clear, nor is the cellular localization of the latter known. We have cloned a cDNA corresponding to the 1.6 kb RNA. This cDNA was sequenced and its complete coding region was subcloned into pSP64. The resulting plasmid was used to direct the synthesis of micrograms of capped RNA transcript after linearization in the 3'-non-coding region. A 39 kDa protein was synthesized when this RNA was translated in rabbit reticulocyte lysate. When this capped RNA was introduced by microinjection into Xenopus oocytes, production of 2-5A synthetase was clearly observed in the cytoplasm and 10-30% of the enzyme accumulated with time in the nucleoplasm. Analysis of cytoplasmic homogenates of these oocytes on a glycerol gradient revealed that the enzyme is fully active in the monomeric form. © 1986.
- Molecular cloning of two genes induced by alpha-interferon in human cells.Publication . Moutschen, S.; Wathelet, M.; Cravador, A.; Defilippi, P.; Huez, G.; Content, J.; Cravador, AlfredoIFN-induce proteins as well as IFN-induced enzymes have been considered for several years as possible mediators of the various interferon actions.
- Molecular cloning, full-length sequence and preliminary characterization of a 56-kDa protein induced by human interferonsPublication . Wathelet, M.; Moutschen, S.; Defilippi, P.; Cravador, A.; Collet, M.; Huez, G.; Content, J.Among the various proteins which are induced when human cells are are treatened with interferon, a predominant protein of unknown function, with molecular mass 56 kDa, has been observed. With the aim of exploring the molecular basis of the regulation of this protein and of its mRNA, in order to understand its biological functionand its possible contribution to the various antiviral and non-antiviral actions exerted by interferons.