Percorrer por autor "Dias, Cristina Barrocas"
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- Comparative study of the antioxidant and enzyme inhibitory activities of two types of Moroccan Euphorbia entire honey and their phenolic extractsPublication . Boutoub, Oumaima; EL-GUENDOUZ, Soukaina; Manhita, Ana; Dias, Cristina Barrocas; Estevinho, Letícia M.; Paula, Vanessa B.; Carlier, Jorge; Costa, Maria Clara; Rodrigues, Brígida; Raposo, Sara; Aazza, Smail; El Ghadraoui, Lahsen; Miguel, Maria GraçaHoney is a natural food product very famous for its health benefits for being an important source of antioxidant and phenolic compounds. Euphorbia honeys obtained from different regions of Morocco were evaluated for their ability to inhibit acetylcholinesterase, lipoxygenase, tyrosinase and xanthine oxidase activities. Their antioxidant properties were evaluated using the: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging capacity, nitric oxide scavenging activity (NO) and scavenging ability of superoxide anion radical. Then, the phenolic extracts of the same entire honey samples were evaluated by liquid chromatography coupled to diode array detection and mass spectrometry (LC-DAD-MS) and tested for the biological activities previously evaluated on the entire honeys, in order to conduct a comparative study between both (honey and phenolic extracts). The chromatographic profiles for the studied Euphorbia honey extracts were different. Phenolic compounds gallic acid, 4-hydroxybenzoic acid and p-coumaric acid were detected in all samples, whereas kampferol was only present in two samples. Physicochemical parameters and total phenolic content were also determined. Entire honey that recorded the highest rate of phenols was sample M6 (E. resinifera) = 69.25 mg GAE/100 g. On the other hand, the phenolic extracts had better antioxidant and enzyme inhibitory activities than the entire honeys, regardless the monofloral honey type. In conclusion, the studied Euphorbia honeys may have a great potential as antioxidant, anti-inflammatory and anti-tyrosinase sources for pharmaceutical and cosmetic applications.
- Estratégias alimentares dos animais domésticos do povoado de Leceia (Oeiras, Portugal) durante a transição do 4.º para o 3.º milénio a.C.: uma abordagem a partir dos isótopos estáveisPublication . Navarrete, Vanessa; Cardoso, João Luis; Dias, Cristina Barrocas; Detry, Cleia; Curto, Ana; Waterman, Anna J.; Wright, Elizabeth; Maurer, Anne‑FranceIn this article, we analyse faunal samples from the Late Neolithic (Layer 4) and Early Chalcolithic (Layer 3) levels of the fortified settlement of Leceia (Oeiras, Portugal) in order to understand the management of the feeding of domestic animals recovered in these excavations. Through the analyses of stable isotopes of δ13C and δ15N in bone collagen, we aim to characterise the grazing systems and the maintenance strategies of the livestock that were consumed and raised in Leceia, in an attempt to obtain a further perspective on the economic complexity of the fortified settlements that emerged during the 3rd millennium in Portuguese Extremadura. The results show the possible use of forage to feed the animals and also seem to indicate the use of fertilised fields. This demonstrates that animal production is already based on intentional practices of animal and land management, as well as on complex exchange networks.
- Your horse is a donkey! Identifying domesticated equids from Western Iberia using collagen fingerprintingPublication . Paladugu, Roshan; Richter, Kristine Korzow; Valente, Maria João; Gabriel, Sónia; Detry, Cleia; Warinner, Christina; Dias, Cristina BarrocasSkeletal remains of two equid species, Equus caballus (horse) and Equus asinus (donkey), have been found in archaeological contexts throughout Iberia since the Palaeolithic and Chalcolithic periods, respectively. These two species play different economic and cultural roles, and therefore it is important to be able to distinguish between the two species to better understand their relative importance in the past human societies. The most reliable morphological features for distinguishing between the two domesticated equids are based on cranial measure-ments and tooth enamel folds, leading to only a small percentage of archaeological remains that can be identified to species. Ancient DNA (aDNA) analysis can be used to reliably distinguish the two equids, but it can be cost prohibitive to apply to large assemblages, and aDNA preservation of non-cranial elements is often low. Collagen peptide mass fingerprinting by matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry, also known as zooarchaeology by mass spectrometry (ZooMS), is a minimally destructive and cost-effective alternative to aDNA analysis for taxonomic determination. However, current ZooMS markers lack resolution below the genus level Equus. In this paper, we report a novel ZooMS peptide marker that reliably distinguishes between horses and donkeys using the enzyme chymotrypsin. We apply this peptide marker to taxonomically identify bones from the Iberian Peninsula ranging from the Iron Age to the Late Modern Period. The peptide biomarker has the potential to facilitate the collection of morphological data for zooarchaeological studies of equids in Iberia and throughout Eurasia and Africa.