Browsing by Author "El Otmani, Fatima"
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- New insight into the chemical composition, antimicrobial and synergistic effects of the Moroccan endemic Thymus atlanticus (Ball) roussine essential oil in combination with conventional antibioticsPublication . Nafis, Ahmed; Iriti, Marcello; Ouchari, Lahcen; El Otmani, Fatima; Marraiki, Najat; Elgorban, Abdallah M.; Syed, Asad; Mezrioui, Noureddine; Hassani, Lahcen; Custódio, LuísaThis study reported the volatile profile, the antimicrobial activity and the synergistic potential of essential oil (EO) from the Moroccan endemic Thymus atlanticus (Ball) Roussine, in combination with the antibiotics ciprofloxacin and fluconazole for the first time, to the best of our knowledge. The EO chemical composition was determined by gas chromatography coupled to mass spectrometry (GC-MS) analysis and the antimicrobial activity assessed by the disc diffusion method against three Gram positive (Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus) and three Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli and one clinical isolate, Klebsiella pneumonia). The antifungal activity was evaluated in four pathogenic yeasts (Candida albicans, C. glabrata, C. krusei and C. parapsilosis). The minimum inhibition concentration (MIC) and the synergistic effect with ciprofloxacin and fluconazole were determined by the two-fold dilution technique and checkerboard test, respectively. Twenty-one constituents were identified by GC-MS in the EO, including carvacrol (21.62%) and borneol (21.13%) as the major components. The EO exhibited a significant antimicrobial activity with inhibition zones ranging from 0.7 mm to 22 mm for P. aeruginosa and B. subtilis, respectively, and MIC values varying from 0.56 mg/mL to 4.47 mg/mL. The fractional inhibitory concentration index (FICI) values ranged from 0.25 to 0.50 for bacteria and from 0.25 to 0.28 for yeasts. The maximum synergistic effect was observed for K. pneumonia with a 256-fold gain of antibiotic MIC. Our results have suggested that EO from T. atlanticus may be used alone or in association with antibiotics as a new potential alternative to prevent and control the emergence of resistant microbial strains both in the medical field and in the food industry.
- The ocean sampling day consortiumPublication . Kopf, Anna; Bicak, Mesude; Kottmann, Renzo; Schnetzer, Julia; Kostadinov, Ivaylo; Lehmann, Katja; Fernandez-Guerra, Antonio; Jeanthon, Christian; Rahav, Eyal; Ullrich, Matthias; Wichels, Antje; Jones, Scott; Orlic, Sandi; Steinke, Michael; Busch, Julia; Duarte, Bernardo; Caçador, Isabel; ten Hoopen, Petra; Canning-Clode, João; Aguirre-Macedo, Ma L.; Bobrova, Oleksandra; Vezzi, Alessandro; Marteinsson, Viggo; Collin, Rachel; Reynisson, Eyjolfur; Loureiro, Clara M.; Luna, Gian M.; Quero, Grazia M.; Löscher, Carolin R.; Kremp, Anke; Amaral, Valentina; DeLorenzo, Marie E.; Yoshida, Takashi; Øvreås, Lise; Wang, Shiao; Fuhrman, Jed A.; Tolman, Jennifer; LaRoche, Julie; Penna, Antonella; Frischer, Marc; Davis, Timothy; Katherine, Barker; Meyer, Christopher P.; Ogata, Hiroyuki; Conan, Pascal; Todorova, Nadezhda; Alonso, Cecilia; Stambler, Noga; Goodwin, Kelly; Nyhus, Paul A. F.; Yakimov, Michael M.; Santana, Rafael; Baltar, Federico; Bodrossy, Levente; Ingleton, Tim; Van De Kamp, Jodie; Frampton, Dion M.; Ostrowski, Martin; Van Ruth, Paul; Karamfilov, Ventzislav; Malthouse, Paul; Bizsel, Kemal C.; Claus, Simon; Deneudt, Klaas; Pedrotti, Maria L.; Munnik, Kate; Mortelmans, Jonas; Pitois, Sophie; Wallom, David; Salter, Ian; Costa, Rodrigo; Schroeder, Declan C.; Kandil, Mahrous M.; Rodriguez-Ezpeleta, Naiara; Kotoulas, Georgios; Berteaux-Lecellier, Veronique; Cochrane, Guy; Wecker, Patricia; Cariou, Thierry; Cancio, I.; Lauro, Federico M.; Vaulot, Daniel; Bienhold, Christina; Ghazal, Hassan; Chaouni, Bouchra; Essayeh, Soumya; Ettamimi, Sara; Iriberri, Juan; Zaid, El H.; Golyshin, Peter N.; Boukhatem, Noureddine; L’Haridon, Stephane; Martin, Patrick; Bouali, Abderrahim; Chahboune, Rajaa; Barrijal, Said; Timinouni, Mohammed; El Otmani, Fatima; Bennani, Mohamed; Mea, Marianna; Gasol, Josep M.; Jensen, Rachelle M.; Gerdts, Gunnar; Hinks, Jamie; Gebbels, Susan; Rosselli, Riccardo; Jude-Lemeilleur, Florence; De Pascale, Fabio; Bente, Edvardsen; Schiavon, Riccardo; dos Santos, Antonina; Moncheva, Snejana; Villar, Emilie; Pesant, Stéphane; Cataletto, Bruno; Malfatti, Francesca; Polymenakou, Paraskevi; Edirisinghe, Ranjith; Sonnenschein, Eva C.; Silveira, Jorge A. H.; Barbier, Michele; Karlsen, Hans E.; Dzhembekova, Nina; Turk, Valentina; Tinta, Tinkara; Fuller, Wayne J.; Salihoglu, Ilkay; Serakinci, Nedime; Ergoren, Mahmut C.; Bresnan, Eileen; Johnson, Zackary; Ramos, Sandra; Sinigalliano, Christopher D.; Siam, Rania; Gidley, Maribeth L.; Biancalana, Florencia; Zingone, Adriana; O’Gara, Fergal; Danovaro, Roberto; Tsiamis, George; Clark, M. S.; Costa, Ana C.; El Bour, Monia; Martins, Ana M.; Magalhães, Catarina; Collins, R. E.; Poulton, Nicole; Ducluzeau, Anne-Lise; Abdallah, Rehab Z.; Jackson, Stephen; Martinez, Jonathan; Costello, Mark J.; Amaral-Zettler, Linda A.; Gilbert, Jack A.; Davies, Neil; Field, Dawn; Glöckner, Frank O.Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world’s oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits.