Browsing by Author "Eldahshan, Omayma A."
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- Functional constituents of Colchicum lingulatum Boiss. & Spruner subsp. Rigescens K. Perss. Extracts and their biological activities with different perspectivesPublication . Yagi, Sakina; Zengin, Gokhan; Eldahshan, Omayma A.; Singab, Abdel Nasser B.; Selvi, Selami; Cetiz, Mehmet Veysi; Rodrigues, Maria João; Custódio, Luísa; Dall’Acqua, Stefano; Elhawary, Esraa A.The genus Colchicum comprises medicinal plants with important bioactive alkaloids, mainly colchicine, and phenolics. The present study was aimed to determine for the first time the phytoconstituents, antioxidant, enzyme inhibition and cytotoxic properties of corms of C. lingulatum. Different solvents extracts (hexane, ethyl acetate, methanol and water) were obtained. The extracts were examined for chemical characterization, antioxidant, enzyme inhibition and cytotoxic properties. To gain more insights, in silico and network pharmacological analysis were performed. Results of GC/MS analysis of the hexane extract revealed that the extract was dominated by oxygenated diterpenes (37%). UHPLC/MS analysis of ethyl acetate, methanol and aqueous extracts indicated the presence of flavonoids as the most abundant class in addition to phenolic acids and alkaloids. The alkaloid colchicine together with its derivatives colchiceine, colchiciline and isomers were detected in the methanol and aqueous extracts. Results of antioxidant activity showed that the methanol extract exhibited the highest DPPH radical scavenging. At 100 mu g/mL, the ethyl acetate, methanol and aqueous extracts displayed potent cytotoxicity against the human embryonic (HEK 293), murine macrophages (RAW 264.7). In silico analyzes have investigated the potential of C. lingulatum in cancer therapy using network pharmacology and molecular docking methods. These results demonstrate C. lingulatum can be a valuable source of bioactive compounds in the development of functional applications including nutraceuticals.
- Small steps to the big picture for health‐promoting applications through the use of chickweed (Stellariamedia): In vitro, in silico, and pharmacologicalnetwork approachesPublication . Cusumano, Gaia; Angeles Flores, Giancarlo; Cetiz, Mehmet Veysi; Kurt, Umran; Ak, Gunes; Saka, Enver; Aly, Shaza H.; Eldahshan, Omayma A.; Singab, Abdel Nasser; Zengin, Gokhan; Senkardes, Ismail; Rodrigues, Maria J.; Custódio, Luísa; Emiliani, Carla; Angelini, PaolaStellaria media L., also called chickweed, is widespread in all parts of the world. In the present study, we investigated the bio-logical properties and chemical profiles of different extracts (ethyl acetate, ethanol, ethanol/water, and water) of S. media. Thechemical profiles were examined using UHPLC/MS/MS technique. Regarding the biological properties, antioxidant propertiesas well as enzyme-inhibiting and cytotoxic effects of the extracts were demonstrated by in vitro methods. To obtain further in-formation about the structure-ability relationship, network pharmacology and molecular docking were also performed. Twelvephenolic compounds were identified in the extracts and most of them were flavonoids (apigenin, kaempferol derivatives, etc.).The water extract showed the best free radical scavenging activity, while the ethanol was the most active in reducing power tests.When inhibiting AChE, the ethyl acetate extract showed the best inhibitory effect. The water extract has a good cytotoxic effecton HepG2 (cell viability: 33.9% at a concentration of 100 g/mL). The analysis, performed using the STRING database, includedthese 45 cancer-associated targets. The identified hub genes were TP53, CDKN2A, PTEN, KRAS, and HRAS. In moleculardocking analysis, acacetin- O-hexoside- O-deoxyhexoside and napigenin-7- O-hexoside exhibit remarkable binding energies withproteins. Consequently, S. media can be potential raw materials for designing functional formulations in the pharmaceutical,nutraceutical, and cosmeceutical industries.