Browsing by Author "García-Barrera, Tamara"
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- Metabolic signatures associated with environmental pollution by metals in Doñana National Park using P. clarkii as bioindicatorPublication . Gago-Tinoco, Amanda; González-Domínguez, Raúl; García-Barrera, Tamara; Blasco-Moreno, Julián; Bebianno, Maria João; Gómez-Ariza, José-LuisBioindicators can reflect the effects of pollutants on their metabolism, being widely used to assess environmental stress. In this sense, the crab Procambarus clarkii has been previously proposed to monitor the contamination in Doñana National Park (southwest Spain) using conventional biomarkers. In this work, a metabolomic approach based on direct infusion mass spectrometry, which allows an easy and quick study of a large number of metabolites in a single run, was used for pollution assessment of this area, considering the biological response of this organism to contamination. In addition, metal accumulation in crab tissues was determined. Thus, the integrated analysis of metabolomic and metallomic data enabled the study of metabolic response of the organism against pollution. Several metabolites were discovered as potential biomarkers of pollution, such as decreased levels of carnosine, alanine, niacinamide, acetoacetate, pantothenic acid, ascorbate, glucose-6-phosphate, arginine, glucose, lactate, phospholipids, and tryglicerides, as well as elevated levels of acetyl carnitine, phosphocholine, choline, and uric acid. In this way, metal-induced toxicity could be related to metabolic impairments, principally oxidative stress, metabolic dysfunction, and dyslipidemia.
- Trace metal concentrations in sediments from the southwest of the Iberian PeninsulaPublication . Blasco, Julián; Gomes, Tânia; García-Barrera, Tamara; Rodríguez-Romero, Araceli; Gonzalez-Rey, M.; Morán-Roldán, Fernando; Tromibini, Chiara; Miotk, Michal; Gómez-Ariza, José Luis; Bebianno, Maria JoãoMetal sediment concentrations (As, Cd, Cu, Hg, Ni, Pb and Zn) in surface sediments (0 to 5 cm) from 11 sampling stations in the southwest of the Iberian Peninsula (the Ria Formosa and the Guadiana River, the Ría de Huelva and the Bay of Cadiz) were determined in two seasonal periods (autumn 2006 and spring 2007). No seasonal metal concentration pattern was identified, as similar values were found in both periods. The highest concentrations of copper, zinc, lead and cadmium were reported in the Ria of Huelva area, and those of mercury and nickel in the Bay of Cadiz. The hazard potential index (HPiM) was determined to assess the potential risk associated with metal contamination (Cu, Cd, Pb and Zn), taking into account extractable (presumed bioavailable) fractions of these metals and site-specific sediment quality values. When the HPiM is >1 there is risk, and according to this index Ría de Huelva is polluted whereas the Bay of Cadiz is not.