Browsing by Author "Garrido, Diego"
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- Effect of of Artemia inherent fatty acid metabolism on the bioavailability of essential fatty acids for Octopus vulgaris paralarvae developmentPublication . B Reis, Diana; Acosta, Nieves G.; Almansa, Eduardo; Garrido, Diego; Andrade, José Pedro; Sykes, Antonio V.; Rodriguez, CovadongaThe aim of the present study was to determine the effect of Artemia metanauplii endogenous fatty acid (FA) metabolism in the actual availability of dietary essential fatty acids (EFA) for Octopus vulgaris paralarvae development. To this end, both Artemia metanauplii inherent radiolabelled FA metabolism and the Octopus vulgaris paralarvae FA metabolism, after being fed with Artemia metanauplii incubated with radiolabelled. FAs, were determined. Metanauplii were incubated in tissue culture plates during 12 h, with 0.3 mu M of a [1-C-14]FA, including either 18:3n-3, 20:4n-6 (ARA), 20:5n-3 (EPA) or 22:6n-3 (DHA), added individually to separate wells as their potassium salts bound to bovine serum albumin. A total of 3600 one-day-old paralarvae were reared up to 24 h in order to ensure the ingestion of a minimum amount of the labelled Artemia. Paralarvae rearing was performed in 4 L cylinder conical tanks at a density of 25 paralarvae L-1. Labelled Artemia metanauplii were added to each paralarvae rearing tank according to a specific [1-C-14]FA treatment. Artemia in vivo metabolism results showed a preferential catabolism of DHA, which was translated into (1) the lower incorporation of this FA into Artemia total lipids (TL)
- Meta-analysis approach to the effects of live prey on the growth of octopus vulgaris paralarvae under culture conditionsPublication . Garrido, Diego; Martín, Virginia M.; Rodríguez, Covadonga; Iglesias, José; Navarro, Juan C.; Reis, Diana; Riera, Rodrigo; Sykes, António V.; Almansa, EduardoThe common octopus (Octopus vulgaris, Cuvier 1797) is a species with increasing interest for marine aquaculture diversification, given its high growth rate and easy adaptation to captivity, among other positive features (Iglesias et al. 2007, 2014a). However, the massive paralarvae mortalities verified under culture conditions ( 100% in most studies) have hampered its commercial production, therefore making this the main bottleneck for industrial farming. According to several authors (Iglesias et al. 2007, 2014a; Iglesias & Fuentes 2013), the high mortalities could be due to: (i) inadequate and/or unbalanced diets that do not fulfil paralarvae nutritional requirements, (ii) lack of standardized rearing techniques, and (iii) little knowledge about octopus paralarvae physiology and behaviour.
- Preliminary results on light conditions manipulation in Octopus vulgaris (Cuvier, 1797) paralarval rearingPublication . Garrido, Diego; Reis, Diana; Orol, Diego; Gonçalves, Rui A.; Martín, M.; Sykes, António V.; Rodríguez, Covadonga; Felipe, Beatriz; Zheng, Xiaodong; Lagos, Luis; Almansa, EduardoHigh paralarvae mortality is a major bottleneck currently hindering the control over the lifecycle of common octopus (Octopus vulgaris Cuvier, 1797). It is believed that this problem might be related to either zoo-technical and/or nutritional aspects. The present paper is focused on the study of different zoo-technical aspects related to light conditions on the rearing of paralarvae, including the effects of polarization in prey ingestion, the use of a blue filter to simulate natural conditions, and the use of focused light to avoid reflections of the rearing tank’s walls. In the first experiment, O. vulgaris paralarvae ingestion of Artemia sp. and copepods (Tisbe sp.) was assessed under either normal or polarized light. In the second experiment, the effect of a blue filter with natural light or focused artificial light on growth and mortality was assessed over 15 days of rearing. Ingestion rate was not influenced by light polarization. Nonetheless, a significantly higher ingestion of Artemia sp. with respect to copepods was observed. The blue filter promoted the use of natural light conditions in Octopus paralarval culture, while focused light reduced the collision of the paralarvae against the walls. However, no significant differences were found in paralarval growth nor survival.