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Browsing by Author "Georgoudis, A."

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  • A genetic linkage map of the hermaphrodite teleost fish Sparus aurata L.
    Publication . Bargelloni, L.; Franch, R.; Patarnello, T.; Tsalavouta, M.; Sarropoulou, E.; Magoulas, A.; Kotoulas, G.; Chatziplis, D.; Georgoudis, A.; Louro, Bruno; Power, Deborah
    The gilthead sea bream (Sparus aurata L.) is a marine fish of great importance for fisheries and aquaculture. It has also a peculiar sex-determination system, being a protandrous hermaphrodite. Here we report the construction of a first-generation genetic linkage map for S. aurata, based on 204 microsatellite markers. Twenty-six linkage groups (LG) were found. The total map length was 1241.9 cM. The ratio between sex-specific map lengths was 1:1.2 (male:female). Comparison with a preliminary radiation hybrid (RH) map reveals a good concordance, as all markers located in a single LG are located in a single RH group, except for Ad-25 and CId-31. Comparison with the Tetraodon nigroviridis genome revealed a considerable number of evolutionary conserved regions (ECRs) between the two species. The mean size of ECRs was 182 bp (sequence identity 60–90%). Forty-one ECRs have a known chromosomal location in the pufferfish genome. Despite the limited number of anchoring points, significant syntenic relationships were found. The linkage map presented here provides a robust comparative framework for QTL analysis in S. aurata and is a step toward the identification of genetic loci involved both in the determination of economically important traits and in the individual timing of sex reversal.
    2007Conference object Open access Show more
  • Genomic resources for the aquaculture of European sea bass
    Publication . Volckaert, F.; Batargias, C.; Bonhomme, F.; Canario, Adelino V. M.; Chistiakov, D.; Choudhuri, J. V.; Galibert, F.; Georgoudis, A.; Haley, Chris; Hellemans, Bart; Kuhl, H.; Kotoulas, Georgios; Law, A.; Libertini, A.; Magoulas, A.; McAndrew, B. J.; Reinhardt, Richard; Senger, Fabrice; Souche, E.; Tsigenopoulos, C.; Whitaker, H. A.
    The BASSMAP consortium, funded by the European Union, has been set up to improve the understanding of the genome of European sea bass (Dicentrarchus labrax). The specific aim is to locate genes of known function on the physical map and to compare specific regions among perciforms. We have produced an F1 cross of outbred sea bass as mapping panel and a Bacterial Artificial Chromosome library (7× redundancy and 165 kb average insert size). End sequencing of the BAC library is in progress and a radiation hybrid panel is under construction.
    2007Conference object Restricted access Show more