Browsing by Author "Grougnet, Raphaël"
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- Chemical composition and biological screening of the essential oils of Micromeria macrosiphon and M. arganietorum (Lamiaceae)Publication . El Yaagoubi, Mohamed; Mechqoq, Hicham; Ortiz, Sergio; Cavaleiro, Carlos; Lecsö‐Bornet, Marylin; Pereira, Catarina; Rodrigues, Maria Joao; Custódio, Luísa; El Mousadik, Abdelhamid; Picot, Laurent; Kritsanida, Marina; Msanda, Fouad; El Aouad, Noureddine; Grougnet, RaphaëlThe chemical composition and in vitro biological activities of the essential oil (EO) of Micromeria macrosiphon Coss. and M. arganietorum (J. Emb.) R. Morales, two Lamiaceae endemic to south Morocco, were investigated. GC/MS analysis resulted in the identification of 36 metabolites from the EO of M. macrosiphon, 45 from M. arganietorum. Borneol was the major metabolite in both oils and together with related derivatives such as camphor, accounted for 2/3 of the EO of M. macrosiphon, 1/3 of those of M. arganietorum. Pinene and terpinene derivatives were also present in high proportions. From a chemotaxonomic point of view, the composition of the examined samples may be related to those of other species endemic to Macaronesia. Both EOs showed significant toxicity towards liver HepG2 and melanoma B16 4A5 tumor cell lines at 100 mu g/mL; however, they were also cytotoxic towards S17 normal cell lines, with a selectivity index <1. No antibacterial activity was noticed against 52 strains at 100 mu g/mL.
- In vitro anti-trypanosoma cruzi activity of halophytes from Southern Portugal reloaded: a special focus on Sea Fennel (Crithmum maritimum L.)Publication . Pereira, Catarina Guerreiro; Moraes, Carolina Borsoi; Franco, Caio H.; Feltrin, Clarissa; Grougnet, Raphaël; Barbosa, Euzébio Guimarães; Panciera, Michele; Correia, Carlos Roque D.; Rodrigues, Maria Joao; L, CustódioMarine halophytes are an outstanding reservoir of natural products and several species have anti-infectious traditional uses. However, reports about their potential use against neglected tropical ailments, such as Chagas disease, are scarce. This work evaluated for the first time the in vitro anti-Trypanosoma cruzi activity of extracts from the aromatic and medicinal species Helichrysum italicum subsp. picardii (Boiss. & Reut.) Franco (Asteraceae, everlasting) and Crithmum maritimum L. (Apiaceae, sea fennel). For that purpose, decoctions, tinctures, and essential oils from everlasting’s flowers and sea fennel’s stems, leaves, and flowers were tested against intracellular amastigotes of two T. cruzi strains. The extract from the sea fennel flower decoction displayed significant anti-trypanosomal activity and no toxicity towards the host cell (EC50 = 17.7 µg/mL, selectivity index > 5.65). Subsequent fractionation of this extract afforded 5 fractions that were re-tested in the same model of anti-parasitic activity. Fraction 1 was the most active and selective (EC50 = 0.47 µg/mL, selectivity index = 59.6) and was submitted to preparative thin-layer chromatography. One major compound was identified, falcarindiol, which was likely the one responsible for the observed antitrypanosomal activity. This was confirmed using a commercially sourced molecule. Target-fishing studies showed falcarindiol as a ligand of T. cruzi spermidine synthase, pointing to a potential enzyme-inhibiting anti-trypanosomal mechanism of action. Overall, this work shows that sea fennel can provide effective anti-parasitic molecule(s) with potential pharmacological applications in the treatment of CD.