Browsing by Author "Justo, E. M."
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- Identification of mandarin hybrids by isozyme and RAPD analysisPublication . Elisiário, P. J.; Justo, E. M.; Leitão, J. M.A cross between the monoembryonic Clementine MA3 SRA 649 used as seed parent and the polyembryonic Carvalhais mandarin used as pollen progenitor was carried out in the SRA INRA-CIRAD, S. Giuliano, Corsica. Cuttings of 61 plants putatively resulting from this cross were sent to the Centro de Citricultura, Fare, Portugal for further agronomic evaluation under field trials. Isozyme analysis allowed the identification of 59 plants as having sexual origin, however, two plants that showed isozyme patterns identical to their seed parent were impossible to identify as being either of zygotic or of somatic origin. Isozymes confirmed Carvalhais mandarin as the pollen parent of 42 progeny plants, while RAPD analysis confirmed Carvalhais paternity over the remaining 19 plants. A fast and low pollutant method for extraction of Citrus total genomic DNA suitable for RAPD analysis is reported. (C) 1999 Elsevier Science B.V. All rights reserved.
- Molecular markers in genetic variability assessment, cultivar fingerprinting and hybrid identification in fruit and vegetablesPublication . Leitão, José; Elisiario, P. J.; Cabrita, L. F.; Justo, E. M.; Pereira, G. M.; Carlier, Jorge; Farinho, M. J.Isozyme, RADP and AFLP markers were used in the UCTA, University of Algarve, for several studies in fruit tree and vegetable species. In Citrus, isozyme analysis was used for assessing genetic variability of traditional cultivars, for distinguishing nucellars from zigotics scion and rootstock cultivars, for parentage determination of hybrid plants and to identify mistakes occurring in commercial nurseries. In figs (Ficus carica L.), isozyme and RAPD analyses allowed the detection of errors in a field collection, and jointly with AFLP analysis were used to discriminate and fingerprint germplasm accessions. In almond (Prunus dulcis Webb) isozyme, RAPD and AFLP markers were utilised to discriminate among cultivars. Recently, several programs aiming to identify molecular markers linked to resistance genes and to construct genomic maps in pineapple, Brassica sp., peas and chickpeas, were initiated in our laboratory.