Percorrer por autor "Lescano, Leonardo"
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- Antioxidant activity, enzyme inhibition, photoprotection, cytotoxicity, and phytochemical profiling of sea lavender (Limonium algarvense Erben) seed extracts for dermo-cosmetic usePublication . Lescano, Leonardo; Cziáky, Zoltán; Kurt-Celep, İnci; Zengin, Gökhan; Fernandes, Eliana; Trentin, Riccardo; Guerreiro Pereira, Catarina Alexandra; Custódio, Luísa; Rodrigues, Maria JoãoDespite sea lavender being a medicinal species, research on its seeds' biological properties and chemical composition is unexplored. Thus, this study evaluated the effect of different extraction solvents on the biological activities and chemical profile of greenhouse-cultivated sea lavender seeds, aiming at their potential use as a dermo-cosmetic ingredient. Therefore, ethyl acetate, acetone, ethanol, and water extracts were examined for their antioxidant activity, enzyme inhibition, photoprotection, and cytotoxicity, followed by phytochemical analysis through spectrophotometric methods, further detailed by Ultrahigh-Performance Liquid Chromatography Coupled with Electrospray Ionization Mass/Mass Spectrometry (UHPLC-Esi-MS/MS).ResultsThe water extract demonstrated significant antioxidant activity, evidenced by low half maximal effective concentration (EC50) values in scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, reducing iron and chelating copper (296, 478, 230 and 678 mu g/mL, respectively). The ethanol extract was more effective in inhibiting cosmetic-related enzymes, particularly elastase and hyaluronidase (2.18 and 3.21 mu g/mL, respectively). The water and acetone extracts had the highest sun protection factors (23.2 and 18.9, respectively). All the extracts had nil to weak cytotoxicity (70-120% cell viability) towards mammalian cell lines. The water extract had the highest phenolics and condensed tannins (115 and 78.30 mg/g extract, respectively), while the ethanol contained the most flavonoids (62.73 mg/g extract). UHPLC-ESI-MS/MS analysis identified ethyl gallate, myricetin, rutin, and quercetin as major components of the ethanol extract, whereas myricetin-O-rutinoside isomers are predominant in the water extract.ConclusionsThese findings highlight the potential of greenhouse-cultivated sea lavender seeds as potential dermo-cosmetic ingredients, with ethanol and water extracts demonstrating superior biological activities and chemical profiles, significantly contributing to general skin health and protection.
- In vitro propagation of Limonium algarvense Erben: towards the conservation of a rare and endemic halophytePublication . Custódio, Luísa; Lescano, Leonardo; Martins, Carolina; Rodrigues, Maria JoãoLimonium algarvense, an endemic halophyte from the Southwest Iberian Peninsula, is listed as “Near Threatened” due to habitat degradation caused by climate change and human activities. Its medicinal properties and limited natural propagation make conservation efforts essential. In vitro propagation offers a sustainable ex situ conservation strategy for this species. Shoot tips from greenhouse-germinated seedlings were sterilized and cultured on Murashige and Skoog (MS) medium supplemented with various growth regulators for 2 cycles of multiplication, such as kinetin (KIN) and naphthalene acetic acid (NAA). Rooting was induced using various MS-based media, including supplements like calcium chloride (CaCl₂) and activated charcoal (AC), followed by a gradual acclimatization to ex vitro conditions. Aseptic cultures were successfully established in 75.6% of the explants using 0.1% HgCl₂. The optimal combination for shoot multiplication was 2.5 mg/L KIN+0.1 mg/L NAA, producing 15.7 shoots/explant after two cycles. The highest rooting percentage (67.6%) was obtained with MS medium supplemented with 10 mM CaCl₂, which also led to superior root length, shoot height, and rosette diameter compared to other treatments. However, survival during the acclimatization remained low (13%). This highlights the need for further optimization of the acclimatization phase to improve the ex vitro survival of propagated plants. Overall, the in vitro protocol provides a promising tool for conserving L. algarvense, but improvements are necessary for large-scale conservation efforts.
- Yeast extract elicitation enhances growth and metabolite production in Limonium algarvense callus culturesPublication . Lescano, Leonardo; Cziáky, Zoltán; Custódio, Luísa; Rodrigues, Maria JoãoLimonium algarvense Erben, a medicinal halophyte, holds significant pharmacological promise due to its rich bioactive compound repertoire. This study aimed to establish robust callus cultures as a sustainable, in vitro model for studying the plant's metabolic responses, particularly focusing on synthesising and accumulating primary and secondary metabolites under various elicitation treatments. Callus cultures were initiated from leaf explants on Murashige and Skoog's medium supplemented with 1 mg/L picloram for 4 weeks. Afterwards, callus cultures were subjected to two elicitor treatments, including salicylic acid-SA and yeast extract-YE at 50 and 100 mg/L for four weeks. Water extracts were assessed for their shifts in primary (total soluble sugars and proteins, and proline), and secondary metabolism (total phenolics, flavonoids, and condensed tannins). In addition, a detailed metabolic profiling was conducted using high-performance liquid chromatography with electrospray ionisation mass spectrometry (HPLC-ESI-MS/MS). Elicitation induced significant shifts in the metabolite synthesis of elicited cultures compared to controls. While YE50 markedly increased the callus yield, the total levels of phenolics, flavonoids condensed tannins and total soluble proteins, the SA50 led to the highest increase in proline content. Metabolomic analysis identified 10 metabolites, including 4-hydroxyphenyllactic acid, hydroxybenzoic acid, riboflavin (Vitamin B2), and dihydroferulic acid methyl ester 4-O-sulfate, that were increased in the YE50 elicitation treatment. This suggests that elicitation can effectively enhance the biosynthesis of primary and secondary metabolites in L. algarvense callus cultures, offering great potential for nutritional and medicinal applications.