A carregar...
Publicação
In vitro propagation of Limonium algarvense Erben: towards the conservation of a rare and endemic halophyte
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 1.78 MB | Adobe PDF |
Orientador(es)
Resumo(s)
Limonium algarvense, an endemic halophyte from the Southwest Iberian Peninsula, is listed as “Near Threatened” due to habitat degradation caused by climate change and human activities. Its medicinal properties and limited natural propagation make conservation efforts essential. In vitro propagation offers a sustainable ex situ conservation strategy for this species. Shoot tips from greenhouse-germinated seedlings were sterilized and cultured on Murashige and Skoog (MS) medium supplemented with various growth regulators for 2 cycles of multiplication, such as kinetin (KIN) and naphthalene acetic acid (NAA). Rooting was induced using various MS-based media, including supplements like calcium chloride (CaCl₂) and activated charcoal (AC), followed by a gradual acclimatization to ex vitro conditions. Aseptic cultures were successfully established in 75.6% of the explants using 0.1% HgCl₂. The optimal combination for shoot multiplication was 2.5 mg/L KIN+0.1 mg/L NAA, producing 15.7 shoots/explant after two cycles. The highest rooting percentage (67.6%) was obtained with MS medium supplemented with 10 mM CaCl₂, which also led to superior root length, shoot height, and rosette diameter compared to other treatments. However, survival during the acclimatization remained low (13%). This highlights the need for further optimization of the acclimatization phase to improve the ex vitro survival of propagated plants. Overall, the in vitro protocol provides a promising tool for conserving L. algarvense, but improvements are necessary for large-scale conservation efforts.
Descrição
Palavras-chave
Sea lavender Micropropagation Conservation Plant tissue culture Salt-tolerant plant
Contexto Educativo
Citação
Editora
Springer
Licença CC
Sem licença CC