Browsing by Author "Lopes, D."
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- Comparing p20's RNA silencing suppressing activity among five phylogenetic groups of Citrus Tristeza virusPublication . Marques, N T.; Costa, A. A.; Lopes, D.; Silva, Goncalo; Nolasco, GustavoThe p20 protein encoded by the Citrus Tristeza Virus (CTV) was previously identified as a RNA silencing suppressor. In this study, we analyzed the p20's suppressing activity from five phylogenetic groups of CTV, using the co-infiltration assay of Green fluorescence protein (GFP) gene and the suppressor gene in 16C line Nicotiana benthamiana plants. Green fluorescence, GFP mRNA relative levels and GFP specific siRNAS were compared showing in most cases, only slight differences. Contrary to previous studies, the p20 suppressor was not able to impede neither short range nor systemic spreading of RNA silencing. The suppressor from the phylogenetic group 4 revealed a much reduced activity when compared with the others. At present we still don't know whether this property is a characteristic of this group or an atypical feature due to a unique point mutation. The differences in the symptom type and intensity originated by isolates belonging to the phylogenetic groups assayed could not be related to differences to the p20 suppressor's activity.
- Fátima revisited: an interactive installationPublication . Alves, Ricardo; Madeira, Marisa; Ferrer, Jorge; Costa, Susana; Lopes, D.; Silva, Bruno; Sousa, L.; Martins, J.; Rodrigues, J. M. F.Three very young shepherds, on May 13, 1917, reported seeing “... a lady even brighter than the sun...”, floating a meter or so in the air, near an old oak tree, when they were pasturing their little herd in Cova da Iria, Portugal. The story of OurLady of Fátima has remained one of the most remarkable odes in Portuguese folklore. It is, beyond the religious event in itself, a key episode in the official history and culture of the Portu-guese people. It is a day celebrated every year; more among thefaithful believers, but also in the media and even as a political catchphrase, widespread, recognized and cele-brated, including by the Vatican and several Popes. In this paper, we present two mul-timedia installations where the central figure is Our Ladyof Fátima, following two main ideas: (a) a “door” opens up the possibility to access multiple space-time experiments, and (b) we try to reach into the Portuguese imaginary surrounding the appearance of Our Lady of Fátima, using a female archetype suggesting a “sacred apparition”. Both installations use a Microsoft Kinect sensor to detect the presence of a viewer for trigger-ing the start of the narrative, followed by recognizing different movements of the view-er(s), e.g., moving left, right, forward, backward, etc., to create different flows in the presented narrative –even at some point changing the face of Our Lady of Fátima with the face of the viewer. Both installations use similar hardware and software concepts, except that the first one uses a Rear Projection solution where the narrative is presented by the image of Our Lady in real size and the second uses a Hologram.