Browsing by Author "Matos, José Joaquim Soares Curado de"
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- Bacteroides species in children with type 1 diabetesPublication . Matos, José Joaquim Soares Curado de; Faleiro, LeonorType 1 diabetes mellitus is an autoimmune disease, characterized by the destruction of the pancreatic beta cells leading to insufficient insulin production. Gut dysbiosis, which is associated with unbalanced diversity of microbiota has been linked with type 1 diabetes (T1D). High population levels of bacteria that belong to the phylum Bacteroidetes have been reported in children with T1D, in contrast to healthy children which show higher levels of Firmicutes. In Finnish children, particularly from the city of Turku, at early-onset of seroconversion it was reported a predominance of Bacteroides dorei in their guts. The main objective of this study was to analyse the Bacteroides population of children with T1D from the Algarve region. To achieve this the Bacteroides spp., including B. dorei were isolated and quantified in faecal samples collected from children with established T1D and control children including their healthy siblings. The isolates of B. dorei, B. uniformis and B. xylanisolvens were genotyped by rep-PCR. The levels of Firmicutes, Bacteroidetes, Bifidobacterium spp. and Lactobacillus spp. were determined by real time PCR. Despite the low amount of B. dorei and Lactobacillus found in the Algarve children, there is a similarity between the children in the study groups, although there is some difference in the amount of Lactobacillus.