Percorrer por autor "Matos, Madalena"
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- Culturable yeast diversity associated with industrial cultures of the microalga microchloropsis gaditana and their ability to produce lipids and biosurfactantsPublication . Matos, Madalena; Fernandes, Mónica A.; Costa, Inês; Coelho, Natacha; Santos, Tamara; Rossetto, Veronica; Varela, João; Sá-Correia, IsabelThe marine oleaginous microalga Microchloropsis gaditana (formerly Nannochloropsis gaditana) exhibits a high capacity to thrive in a broad range of environmental conditions, being predominantly utilized as feed in aquaculture. This article reports the characterization of the culturable yeast population present during the scale-up process of M. gaditana cultivation at Necton S.A. facilities, from 5 L flasks until tubular photobioreactors. The 146 yeast isolates obtained, molecularly identified based on D1/D2 and ITS nucleotide sequences, belong to the species Rhodotorula diobovata, R. mucilaginosa, R. taiwanensis, R. sphaerocarpa, Vishniacozyma carnescens, Moesziomyces aphidis, and Meyerozyma guilliermondii. The yeast abundance was found to increase throughout upscaling stages. The yeast populations isolated from microalgal cultures and water samples share phylogenetically close isolates, indicating a possible common source. The impressive high percentage of red yeasts isolated (90%) is consistent with the recognized role of carotenoid pigments in yeast photoprotection. Sixty yeast isolates were tested for lipid (Nile Red staining) and biosurfactant (oil drop dispersion and emulsification index) production. Results revealed that these capacities are common features. Microbial lipids and biosurfactants have promising biotechnological applications. Moreover, biosurfactants can fulfill various physiological roles and provide advantages in natural environments contributing to the promising use of yeasts as probiotics in microalgae production.
- Yeast species associated with industrial cultures of the marine microalgae Tisochrysis lutea: Temperature profiles and auxin productionPublication . Matos, Madalena; Fernandes, Mónica A.; Coelho, Natacha; Santos, Tamara; Varela, João; Rodrigues, Alexandre M. C.; Sá-Correia, IsabelThis study provides the first systematic characterization of culturable yeast diversity associated with large-scale cultivation of Tisochrysis lutea. This marine haptophyte is widely used in aquaculture for its high content of essential fatty acids, pigments, and other bioactive compounds. Culture sampling was conducted at Necton S.A. facilities (Olhão, Portugal) over full production cycles from 5 L flasks until tubular photobioreactors during the months of May and June. The study aimed to identify and isolate the present yeast species and evaluate their physiological traits relevant to potential co-cultivation strategies. All retained isolates belonged to the phylum Basidiomycota, with six species identified: Rhodotorula sphaerocarpa (45%), R. mucilaginosa (20%), R. diobovata (13%), Vishniacozyma carnescens (16%), Naganishia diffluens (3%), and Moesziomyces aphidis (3%). Temperature growth profiles (10–40 °C), tolerance to artificial sea water, and auxin production were characterized, revealing that, except for V. carnescens, the yeast isolates grow optimally at 25–30 °C, within the ideal range for T. lutea cultivation. Results suggest that some of these marine yeasts, particularly R. sphaerocarpa and R. mucilaginosa isolates, could serve as biological enhancers of algal productivity, in situ. This foundational work supports future efforts to develop targeted yeast management or co-cultivation strategies, with the goal of improving biomass yield and metabolite production in industrial T. lutea photobioreactors.
- Yeast species associated with industrial cultures of the marine microalgae tisochrysis lutea: temperature profiles and auxin productionPublication . Matos, Madalena; Fernandes, Mónica A.; Coelho, Natacha; Santos, Tamara; Varela, João; Rodrigues, Alexandre M. C.; Sá-Correia, IsabelThis study provides the first systematic characterization of culturable yeast diversity associated with large-scale cultivation of Tisochrysis lutea. This marine haptophyte is widely used in aquaculture for its high content of essential fatty acids, pigments, and other bioactive compounds. Culture sampling was conducted at Necton S.A. facilities (Olhão, Portugal) over full production cycles from 5 L flasks until tubular photobioreactors during the months of May and June. The study aimed to identify and isolate the present yeast species and evaluate their physiological traits relevant to potential co-cultivation strategies. All retained isolates belonged to the phylum Basidiomycota, with six species identified: Rhodotorula sphaerocarpa (45%), R. mucilaginosa (20%), R. diobovata (13%), Vishniacozyma carnescens (16%), Naganishia diffluens (3%), and Moesziomyces aphidis (3%). Temperature growth profiles (10–40 ◦C), tolerance to artificial sea water, and auxin production were characterized, revealing that, except for V. carnescens, the yeast isolates grow optimally at 25–30 ◦C, within the ideal range for T. lutea cultivation. Results suggest that some of these marine yeasts, particularly R. sphaerocarpa and R. mucilaginosa isolates, could serve as biological enhancers of algal productivity, in situ. This foundational work supports future efforts to develop targeted yeast management or co-cultivation strategies, with the goal of improving biomass yield and metabolite production in industrial T. lutea photobioreactors.