Percorrer por autor "Muhumuza, Geresomu"
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- Applying collocation and APRIORI analyses to chimpanzee diets: methods for investigating nonrandom food combinations in primate self‐medicationPublication . Freymann, Elodie; Coelho, João d'Oliveira; Hobaiter, Catherine; Huffman, Michael A.; Muhumuza, Geresomu; Zuberbühler, Klaus; Carvalho, SusanaIdentifying novel medicinal resources in chimpanzee diets has historically presented challenges, requiring extensive behavioral data collection and health monitoring, accompanied by expensive pharmacological analyses. When putative therapeutic self‐medicative behaviors are observed, these events are often considered isolated occurrences, with little attention paid to other resources ingested in combination. For chimpanzees, medicinal resource combinations could play an important role in maintaining well‐being by tackling different symptoms of an illness, chemically strengthening efficacy of a treatment, or providing prophylactic compounds that prevent future ailments. We call this concept the self‐medicative resource combination hypothesis. However, a dearth of methodological approaches for holistically investigating primate feeding ecology has limited our ability to identify nonrandom resource combinations and explore potential synergistic relationships between medicinal resource candidates. Here we present two analytical tools that test such a hypothesis and demonstrate these approaches on feeding data from the Sonso chimpanzee community in Budongo Forest, Uganda. Using 4 months of data, we establish that both collocation and APRIORI analyses are effective exploratory tools for identifying binary combinations, and that APRIORI is effective for multi‐ item rule associations. We then compare outputs from both methods, finding up to 60% agreement, and propose APRIORI as more effective for studies requiring control over confidence intervals and those investigating nonrandom associations between more than two resources. These analytical tools, which can be extrapolated across the animal kingdom, can provide a cost‐effective and efficient method for targeting resources for further pharmacological investigation, potentially aiding in the discovery of novel medicines.
- Pharmacological and behavioral investigation of putative self-medicative plants in budongo chimpanzee dietsPublication . Freymann, Elodie; Carvalho, Susana; Garbe, Leif A.; Ghazhelia, Dinda Dwi; Hobaiter, Catherine; Huffman, Michael A.; Muhumuza, Geresomu; Schulz, Lena; Sempebwa, Daniel; Wald, Florian; Yikii, Eguma R.; Zuberbühler, Klaus; Schultz, Fabien; Armel Jackson SeukepWild chimpanzees consume a variety of plants to meet their dietary needs and maintain wellbeing. While some plants have obvious value, others are nutritionally poor and/or contain bioactive toxins which make ingestion costly. In some cases, these nutrient-poor resources are speculated to be medicinal, thought to help individuals combat illness. In this study, we observed two habituated chimpanzee communities living in the Budongo Forest, Uganda, and collected 17 botanical samples associated with putative self-medication behaviors (e.g., bark feeding, dead wood eating, and pith-stripping) or events (e.g., when consumer had elevated parasite load, abnormal urinalysis, or injury). In total, we selected plant parts from 13 species (nine trees and four herbaceous plants). Three extracts of different polarities were produced from each sample using n-hexane, ethyl acetate, and methanol/water (9/1, v/v) and introduced to antibacterial and anti-inflammatory in vitro models. Extracts were evaluated for growth inhibition against a panel of multidrug-resistant clinical isolates of bacteria, including ESKAPE strains and cyclooxygenase-2 (COX-2) inhibition activity. Pharmacological results suggest that Budongo chimpanzees consume several species with potent medicinal properties. In the antibacterial library screen, 45 out of 53 extracts (88%) exhibited ≥40% inhibition at a concentration of 256 μg/mL. Of these active extracts, 41 (91%) showed activity at ≤256μg/mL in subsequent dose-response antibacterial experiments. The strongest antibacterial activity was achieved by the n-hexane extract of Alstonia boonei dead wood against Staphylococcus aureus (IC50: 16 μg/mL; MIC: 32 μg/mL) and Enterococcus faecium (IC50: 16 μg/mL; MIC: >256 μg/mL) and by the methanol-water extract of Khaya anthotheca bark and resin against E. faecium (IC50: 16 μg/mL; MIC: 32 μg/mL) and pathogenic Escherichia coli (IC50: 16 μg/mL; MIC: 256 μg/mL). We observed ingestion of both these species by highly parasitized individuals. K. anthotheca bark and resin were also targeted by individuals with indicators of infection and injuries. All plant species negatively affected growth of E. coli. In the anti-inflammatory COX-2 inhibition library screen, 17 out of 51 tested extracts (33%) showed ≥50% COX-2 inhibition at a concentration of 5 μg/mL. Several extracts also exhibited anti-inflammatory effects in COX-2 dose-response experiments.
- Self-directed and prosocial wound care, snare removal, and hygiene behaviors amongst the Budongo chimpanzeesPublication . Freymann, Elodie; Hobaiter, Catherine; Huffman, Michael Alan; Klein, Harmonie; Muhumuza, Geresomu; Reynolds, Vernon; Slania, Nora E.; Soldati, Adrian; Yikii, Eguma Robert; Zuberbühler, Klaus; Carvalho, SusanaUnderstanding the cognitive and social foundations of healthcare behaviors in humans requires examining their evolutionary precursors in our closest living relatives. Investigating self-directed and other-directed healthcare in chimpanzees provides crucial insights into the origins of medicinal knowledge, identification of specific medicinal resources used for health maintenance, and the emergence of prosocial healthcare capacities. Here we document and analyze both previously reported and newly observed instances of selfdirected and other-directed wound care, snare removal, and putatively medicinal hygiene behaviors in the Sonso and Waibira chimpanzee communities of the Budongo Forest in Uganda. Reports of these behaviors come from archival records collected from over thirty years of observation at the Budongo Conservation Field Station (BCFS), videos recorded by researchers at the site, and all-occurrence behavioral data collected over two 4-month periods of direct observation. We describe self-directed wound care behaviors such as wound licking, leaf-dabbing, pressing fingers to wounds, and the application of chewed plant material to wounds, as well as a successful self-directed snare removal. We also document self-directed hygiene behaviors including postcoital genital leaf wiping and post-defecation leaf wiping.