Browsing by Author "Rocha, E."
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- Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis)Publication . Campos, C.; Valente, L. M. P.; Conceição, L. E. C.; Engrola, S.; Sousa, V.; Rocha, E.; Fernandes, J. M. O.Fertilised eggs of Senegalese sole were incubated at 15, 18 or 21 °C, and after hatching all larvae were reared at 21 °C until 30 days post-hatch. By this point larvae from the 18 or 21 °C temperature groups had 11 and 9% more muscle fibres than those from 15 °C, respectively. Hyperplastic growth during metamorphosis was higher in larvae from 18 °C. Embryonic temperature induced gene expression changes, albeit with a variable pattern throughout development. Myf5, myod2, myHC and fst mRNA levels were significantly higher at several stages prior to hatching in embryos incubated at 21 °C, whereas hsp90AB and hsp70 transcripts were present at higher levels in the 15 °C group. Myf5, myod1, myod2, pax7, myog, fst, igf-II, igf1r, hsp90AA and hsp90AB were expressed at higher levels during early development, particularly during somitogenesis. In contrast, mrf4, myHC, mylc2, igf-I, mstn1 and hsp70 were up-regulated at later stages of larval development, namely during and after metamorphosis. This study is the first example of thermal plasticity of myogenesis with prolonged effect in a flatfish.
- Thermal plasticity of muscle cellularity and gene expression in Senegalese sole (Solea senegalensis)Publication . Campos, C.; Valente, L. M. P.; Conceição, L. E. C.; Engrola, S.; Sousa, V.; Rocha, E.; Gavaia, Paulo J.; Fernandes, J. M. O.Temperature-induced responses at the level of muscle growth potential have been observed in teleost fish species, concomitantly with gene expression changes (Johnston and Hall 2004). Incubation temperature has been demonstrated to influence the muscle cellularity at hatch and during larval development, and these effects are highly species-specific. Changes in the relative timing and expression level of myogenic genes were found to be related with thermal plasticity of muscle phenotype (Fernandes, Mackenzie et al. 2006). The eggs of the flatfish Senegalese sole (Solea senegalensis) in aquaculture conditions are normally incubated within the 16-20ºC range, but influence of temperature on muscle cellularity and expression of growth-related genes is unknown. Understanding these effects may contribute to improve growth conditions and juvenile production.