Percorrer por autor "Saka, Enver"
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- Assessing the bioactive potential of Lysimachia atropurpurea extracts using HPLC-MS/MS, in vitro and in silico analysisPublication . Ak, Gunes; Nilofar, Nilofar; Saka, Enver; Uba, Abdullahi Ibrahim; Rodrigues, Maria João; Fernandes, Eliana; Custódio, Luísa; Yildiztugay, Evren; Yapıcı, Ismail; Gulcin, Ilhami; Mahmoud, Orchid A.; Eldahshan, Omayma A.; Singab, Abdel Nasser B.; Wu, Yimao; Li, Meng-Yao; Zengin, GokhanThe genus Lysimachia is of great interest to the scientific community, especially in terms of its potential anticancer effects. In this study, the aerial parts and roots of Lysimachia atropurpurea L. were collected and extracted by maceration using solvents of ethyl acetate (EA), ethanol (EtOH), ethanol/water, and water. The biological activities of the extracts, including antioxidant, enzyme inhibition, and anticancer effects, were evaluated using various assays. High-performance liquid chromatographytandem mass spectrometry (HPLC-MS/MS) analysis revealed a total of 32 compounds in the extracts of L. atropurpurea. The roots showed significantly the highest antioxidant activity compared to the aerial part. In case of cholinesterase inhibition, the aerial parts of the EtOH extract showed the highest acetylcholinesterase (AChE) inhibition activity, measuring 3.05 mg galatamine equivalent (GALAE)/g. The EtOH and EtOH/water extracts exhibited the strongest cytotoxicity, reducing the viability of human neuroblastoma (SH-SY5Y) and human hepatocarcinoma (HepG2) cancer cells to as low as 4.86–6.33 %. The results of network pharmacology and molecular docking suggest that the extract of L. atropurpurea exerts inhibitory effects on hepatocellular carcinoma through the modulation of SRC, PI3K, and HSP90, while it demonstrates potential inhibitory activity against neuroblastoma by targeting SRC, PI3K, HSP90, ESR1, AKT, and other related targets. In conclusion, the L. atropurpurea extracts showed potential antioxidant, enzyme inhibition, and selective anticancer effects, which support their potential for further research as therapeutic agents in drug development.
- Small steps to the big picture for health‐promoting applications through the use of chickweed (Stellariamedia): In vitro, in silico, and pharmacologicalnetwork approachesPublication . Cusumano, Gaia; Angeles Flores, Giancarlo; Cetiz, Mehmet Veysi; Kurt, Umran; Ak, Gunes; Saka, Enver; Aly, Shaza H.; Eldahshan, Omayma A.; Singab, Abdel Nasser; Zengin, Gokhan; Senkardes, Ismail; Rodrigues, Maria J.; Custódio, Luísa; Emiliani, Carla; Angelini, PaolaStellaria media L., also called chickweed, is widespread in all parts of the world. In the present study, we investigated the bio-logical properties and chemical profiles of different extracts (ethyl acetate, ethanol, ethanol/water, and water) of S. media. Thechemical profiles were examined using UHPLC/MS/MS technique. Regarding the biological properties, antioxidant propertiesas well as enzyme-inhibiting and cytotoxic effects of the extracts were demonstrated by in vitro methods. To obtain further in-formation about the structure-ability relationship, network pharmacology and molecular docking were also performed. Twelvephenolic compounds were identified in the extracts and most of them were flavonoids (apigenin, kaempferol derivatives, etc.).The water extract showed the best free radical scavenging activity, while the ethanol was the most active in reducing power tests.When inhibiting AChE, the ethyl acetate extract showed the best inhibitory effect. The water extract has a good cytotoxic effecton HepG2 (cell viability: 33.9% at a concentration of 100 g/mL). The analysis, performed using the STRING database, includedthese 45 cancer-associated targets. The identified hub genes were TP53, CDKN2A, PTEN, KRAS, and HRAS. In moleculardocking analysis, acacetin- O-hexoside- O-deoxyhexoside and napigenin-7- O-hexoside exhibit remarkable binding energies withproteins. Consequently, S. media can be potential raw materials for designing functional formulations in the pharmaceutical,nutraceutical, and cosmeceutical industries.