Browsing by Author "Selvi, Selami"
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- Characterising the metabolomic diversity and biological potentials of extracts from different parts of two cistus species using UHPLC-MS/MS and In vitro techniquesPublication . Ahmed, Shakeel; Zengin, Gokhan; Selvi, Selami; Ak, Gunes; Cziáky, Zoltán; Jekő, József; Rodrigues, Maria João; Custódio, Luísa; Venanzoni, Roberto; Flores, Giancarlo Angeles; Cusumano, Gaia; Angelini, PaolaThis study investigates the biochemical composition and biological properties of different parts (leaves, roots, and twigs) of two Cistus species (Cistus monspeliasis and Cistus parviflorus). The extracts were analysed using UHPLC-MS/MS to determine their chemical profiling. A range of antioxidant assays were performed to evaluate the extract’s antioxidant capabilities. The enzyme inhibition studies focused on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-amylase, and α-glucosidase and tyrosinase. In addition, the study examined the antimicrobial effects on different bacteria and yeasts and evaluated the toxicity using the MTT assay. Quinic acid, citric acid, gallic acid, catechin, quercetin derivatives, kaempferol, myricetin, ellagic acid, prodelphinidins, procyanidins, scopoletin, and flavogallonic acid dilactone are the main bioactive compounds found in both species. In enzyme inhibition assays, C. monspeliasis roots exhibited significant activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), with the values of 2.58 ± 0.02 mg GALAE/g and 11.37 ± 1.93 mg GALAE/g, respectively. Cytotoxicity studies showed mostly weak toxicity, with some samples moderately reducing viability in RAW and HepG2 cells. These findings underscore the diverse biochemical profiles and bioactive potential of Cistus species, suggesting their utility as natural sources of antioxidants and enzyme inhibitors for pharmaceutical and nutraceutical development.
- Functional constituents of Colchicum lingulatum Boiss. & Spruner subsp. Rigescens K. Perss. Extracts and their biological activities with different perspectivesPublication . Yagi, Sakina; Zengin, Gokhan; Eldahshan, Omayma A.; Singab, Abdel Nasser B.; Selvi, Selami; Cetiz, Mehmet Veysi; Rodrigues, Maria João; Custódio, Luísa; Dall’Acqua, Stefano; Elhawary, Esraa A.The genus Colchicum comprises medicinal plants with important bioactive alkaloids, mainly colchicine, and phenolics. The present study was aimed to determine for the first time the phytoconstituents, antioxidant, enzyme inhibition and cytotoxic properties of corms of C. lingulatum. Different solvents extracts (hexane, ethyl acetate, methanol and water) were obtained. The extracts were examined for chemical characterization, antioxidant, enzyme inhibition and cytotoxic properties. To gain more insights, in silico and network pharmacological analysis were performed. Results of GC/MS analysis of the hexane extract revealed that the extract was dominated by oxygenated diterpenes (37%). UHPLC/MS analysis of ethyl acetate, methanol and aqueous extracts indicated the presence of flavonoids as the most abundant class in addition to phenolic acids and alkaloids. The alkaloid colchicine together with its derivatives colchiceine, colchiciline and isomers were detected in the methanol and aqueous extracts. Results of antioxidant activity showed that the methanol extract exhibited the highest DPPH radical scavenging. At 100 mu g/mL, the ethyl acetate, methanol and aqueous extracts displayed potent cytotoxicity against the human embryonic (HEK 293), murine macrophages (RAW 264.7). In silico analyzes have investigated the potential of C. lingulatum in cancer therapy using network pharmacology and molecular docking methods. These results demonstrate C. lingulatum can be a valuable source of bioactive compounds in the development of functional applications including nutraceuticals.