Browsing by Author "Shylova, Anastasiia"
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- An autochthonous aerobic bacterial community and its cultivable isolates capable of degrading fluoxetinePublication . Palma, Tânia Cristina da Luz; Shylova, Anastasiia; Carlier, Jorge; Costa, Maria ClaraBACKGROUND Fluoxetine is an antidepressant and recalcitrant fluorine pharmaceutical that is poorly biodegraded, so it enters the hydric resources and causes hazardous effects to aquatic environments. According to these fluoxetine features, the main aim of the present research was to find resistant bacteria in environmental samples with a high degradation efficiency. RESULTS The results obtained from raw municipal wastewater spiked with fluoxetine and inoculated with aerobic sludge from a Portuguese wastewater treatment plant under highly aerobic conditions showed that more than half and approximate to 89% of the drug was degraded after 48 and 144 h, respectively. During the assay, the initial population (mainly composed of Arcobacter, Bacteroides, and Macellibacteroides) shifted with an increase of members of the Acinetobacter, Rheinheimera, Shewanella, Pseudomonas, Methylobacillus, Piscinobacter genera and Aeromonadales order and the Pseudomonadaceae family, all of which were likely responsible for fluoxetine biodegradation. From the same sludge, six bacterial isolates were selected and identified as follows: Pseudomonas putida, Enterobacter ludwigii, Pseudomonas nitritireducens, Alcaligenes faecalis, Pseudomonas aeruginosa, and Pseudomonas nitroreducens; all of them grew with fluoxetine as sole carbon source. Pseudomonas nitroreducens showed the highest removal of 55 +/- 1% at 20 mg L-1 fluoxetine after 24 h. CONCLUSION An autochthonous aerobic bacterial community and its cultivable isolates showed the capacity to biodegrade fluoxetine. Biodegradation, rather than adsorption, appears to play the main role in the fluoxetine removal in aerobic conditions using bacteria simply obtained from an environmental sample. As far as is known, those bacteria are reported for the first time as fluoxetine biodegraders; thus, these bacteria are a promising option to integrate into new bioremediation processes aiming at the removal of fluoxetine.
- Biodegradation of fluoxetine and 17á-ethinylestradiol by bacteria isolated from a wastewater treatment plant processPublication . Shylova, Anastasiia; Costa, Maria Clara; Palma, Tania Cristina da LuzThe increasing development of drugs such as antidepressants and hormones led to serious environmental and health problems, becoming mandatory to study different ways to remove a selective serotonin re-uptake inhibitor like fluoxetine (FLX) and estrogens like 17α- ethinylestradiol (EE2) during the wastewater treatment process. The study was carried out by using activated sludge samples from the Faro Northwest wastewater treatment plant (WWTP) to isolate bacteria under aerobic conditions in order to test their resistance in the presence of FLX and EE2 and investigate the biodegradability of chosen pharmaceuticals. In the present study, the identification of bacterial isolates was performed by sequencing the 16S rRNA gene of the obtained resistant bacteria. The obtained isolates which showed the ability to grow in the presence of 20 and 50 mg/L FLX were Pseudomonas putida, Enterobacter ludwigii, Pseudomonas nitritireducens, Alcaligenes faecalis, Pseudomonas aeruginosa, Pseudomonas nitroreducens and in the presence of 15 mg/L EE2 were Acinetobacter bouvetii, Acinetobacter kookii, Pantoea agglomerans Shinella zoogloeoides. These isolates were identified as FLX and EE2 degraders for the first time. FLX and EE2 biodegradation assays was performed in liquid medium. The results showed that for 20 mg/L FLX the bacterial isolate Pseudomonas nitroreducens displayed the highest removal efficiency of 55 ± 1% and for 15 mg/L EE2 Pantoea agglomerans removed 64 ± 4% of the drug. This research shows the importance of the involved bacteria for the effectiveness of the removal of these drugs in a wastewater treatment plant. A complete removal of pharmaceutical compounds from wastewater will stimulate water reuse, directing attention worldwide increasing requirements for clean and safe fresh water.