Browsing by Author "Sinan, Kouadio Ibrahime"
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- A comparative study on biological properties and chemical profiles of different solvent extracts from Centaurea bingoelensis, an endemic plant of TurkeyPublication . Uysal, Ahmet; Zengin, Gokhan; Mahomoodally, Mohamad Fawzi; Picot-Allain, Carene; Jeko, Jozsef; Cziaky, Zoltan; Rodrigues, Maria Joao; Ak, Gunes; Polat, Ridvan; Urusan, Zeynep; Sinan, Kouadio Ibrahime; Custódio, LuísaThis study focused on the biological evaluation and chemical characterization of Centaurea bingoelensis, an endemic plant to Turkey. Different solvent extracts (hexane, dichloromethane (DCM), ethyl acetate (EA), methanol, hydro-methanol, and water extracts) were prepared. The antioxidant, enzyme inhibitory, antimicrobial and cytotoxic activities were investigated. The hydro-methanol extract, possessing high phenolic content (41.57 mg gallic acid equivalent (GAE)/g extract), showed highest reducing capacity (136.87 and 82.16 mg Trolox equivalent [TE]/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant power, respectively) and radical scavenging potential (70.72 and 76.53 mg TE/g extract, against 2,2-diphenyl-1-picryl-hydrazyl and 2,2' -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), respectively). Ultra performance liquid chromatography - tandem mass spectrometer (UHPLC/MS/MS) analysis revealed the presence of 67 and 62 compounds in the methanolic and water extracts, respectively. According to the broth microdilution results, DCM and EA extracts showed significant antimicrobial activity with Sarcina lutea being the most sensitive bacterium to the extracts (MIC = 0.19 (DCM) and 0.097 mg/mL (EA)). Most extracts showed promising antifungal activity against Candida albicans. The methanol extract was most toxic against human hepatocarcinoma (HepG2) cells, and also significantly reduced the cell viability of the non-tumoral murine bone marrow stromal (S17) cells. This study has provided valuable scientific data on this endemic plant which can be considered as a promising candidate for the development of novel phyto-pharmaceuticals.
- Chemical characterization, cytotoxic, antioxidant, antimicrobial, and enzyme inhibitory effects of different extracts from one sage (Salvia ceratophylla L.) from Turkey: open a new window on industrial purposesPublication . Uysal, Sengul; Zengin, Gokhan; Sinan, Kouadio Ibrahime; Ak, Gunes; Ceylan, Ramazan; Mahomoodally, Mohamad Fawzi; Uysal, Ahmet; Sadeer, Nabeelah Bibi; Jekő, József; Cziáky, Zoltán; Rodrigues, Maria Joao; Yıldıztugay, Evren; Elbasan, Fevzi; Custódio, LuísaIn the present study, the methanolic, hydro-methanolic, dichloromethane, hexane and aqueous extracts of Salvia ceratophylla L. (Family: Lamiaceae), a lemon-scented herb, were tested for total phenolic (TPC) and flavonoid content (TFC) and antioxidant activities were evaluated using a battery of assays (2,2-diphenyl-1- picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity, total antioxidant capacity (TAC) (phosphomolybdenum) and metal chelating). Enzyme inhibitory effects were investigated using acetyl- (AChE), butyryl-cholinesterase (BChE), tyrosinase, a-amylase and a-glucosidase as target enzymes. Regarding the cytotoxic abilities, HepG2, B164A5 and S17 cell lines were used. The phytochemical profile was conducted using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Our data showed that the methanolic aerial extracts possessed the highest phenolic (72.50 0.63 mg gallic acid equivalent per g) and flavonoid (43.77 1.09 mg rutin equivalent per g) contents. The hydromethanolic aerial extract showed significant DPPH radical scavenging activity (193.40 0.27 mg TE per g) and the highest reducing potential against CUPRAC (377.93 2.38 mg TE per g). The best tyrosinase activity was observed with dichloromethane root extract (125.45 1.41 mg kojic acid equivalent per g). Among the tested extracts, hexane root extract exerted the highest antimicrobial potential with a minimum inhibitory concentration value of 0.048 mg mL 1. Methanolic root extract showed the lowest cytotoxicity (28%) against HepG2 cells. Phytochemical analysis revealed the presence of important polyphenolic compounds including luteolin, gallic acid, rosmarinic acid, to name a few. This research can be used as one methodological starting point for further investigations on this lemon-scented herb.
- Chemical profile, antioxidant, antimicrobial, enzyme inhibitory, and cytotoxicity of seven Apiaceae species from Turkey: a comparative studyPublication . Zengin, Gokhan; Sinan, Kouadio Ibrahime; Ak, Gunes; Mahomoodally, Mohamad Fawzi; Paksoy, Mehmet Yavuz; Picot-Allain, Carene; Glamocilja, Jasmina; Sokovic, Marina; Jeko, Jozsef; Cziaky, Zoltan; Rodrigues, Maria Joao; Pereira, Catarina; Custódio, LuísaSeveral Apiaceae species, used as both food and in complementary and alternative medicine, represents a rich source of potential valuable phytopharmaceuticals which necessitates scientific contemplation. In the present study, the antioxidant, enzyme inhibitory, antimicrobial, and cytotoxic properties of methanol extracts of seven Apiaceae species, (Chaerophyllum macrospermum (Willd. ex Spreng.) Fisch. & C.A.Mey. ex Hohen, Ferula rigidula Fisch. ex DC., Ferula orientalis L., Prangos ferulacea Lindl., Prangos peucedanifolia Fenzl., Ferulago setifolia K. Koch, and Pimpinella anthriscoides Boiss.) were evaluated. Species belonging to the Prangos genus exhibited the highest total phenolic content, namely P. peucedanifolia and P. ferulacea, with values of 47.90 and 44.44 mg gallic acid equivalent/g extract, respectively. P. peucedanifolia also displayed the highest radical scavenging capacity (81.53 and 102.70 mg Trolox equivalent [TE]/g extract for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), respectively) and reducing power (165.87 and 100.09 mg TE/g extract for cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP), respectively). C. macrospermum showed the most potent inhibition against Alzheimer's disease related enzymes, namely acetylcholinesterase (4.53 mg galantamine equivalent [GALAE]/g extract) and butyrylcholinesterase (3.22 mg GALAE/g extract). P. ferulacea (131.94 mg kojic acid (KAE) equivalent/g extract) and P. peucedanifolia (4.97 mmol acarbose equivalent (ACAE)/g extract) were potent inhibitors of tyrosinase and a-glucosidase, respectively. In general, studied species were able to reduce cellular viabilities. P. peucedanifolia possessed promising antibacterial potential against Bacillus cereus (Minimum inhibition concentration (MIC): 0.37 mg/mL), L. monocytogenes (MIC: 0.56 mg/mL), P. aeruginosa and Escherichia coli (MIC: 0.27 mg/mL), Salmonella typhimurium and Enterobacter cloacae (MIC: 0.75 mg/mL). F. rigidula showed the highest antifungal effect against Aspergillus ochraceus and Trichoderma viride (MIC: 0.10 mg/mL). The present findings could be the scientific starting point towards the pharmaceutical and/or commercial utilization of these Apiaceae species.
- Phytochemical characterization and bioactivities of five Apiaceae species: Natural sources for novel ingredientsPublication . Zengin, Gokhan; Mahomoodally, Mohamad Fawzi; Paksoy, Mehmet Yavuz; Picot-Allain, Carene; Glamocilja, Jasmina; Sokovic, Marina; Diuzheva, Alina; Jeko, Jozsef; Cziaky, Zoltan; Rodrigues, Maria Joao; Sinan, Kouadio Ibrahime; Custódio, LuísaSeveral species of the Apiaceae family have been employed in traditional cultures for their curative virtues. The present study focused on five Apiaceae species, (Falcaria vulgaris (FV), Smyrniopsis aucher( (SA), Smyrniopsis mtutzurdagensis (SM), Smyrnium cordifolium (SC), and Actinolema macrolema (AM)). The antioxidant, enzyme inhibitory (alpha-amylase, alpha-glucosidase, acetyl- and butyrylcholinesterase, lipase, and tyrosinase), antimicrobial, phytochemical, and cytotoxicity profiles of the methanol extracts of the selected Apiaceae species were determined. SC extract (35.68 mg gallic acid equivalent/g extract) possessed the highest phenolic content while the AM extract (56.79 mg rutin equivalent/g extract) had the highest flavonoid content. HPLC-ESI-MS (High performance liquid chromatography-electrospray tandem mass spectrometry) analyses showed presence of ferulic acid in all the five species. SC extract exhibited high radical scavenging (59.28 and 94.31 mg Trolox equivalent [TE]/g extract, DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid), respectively) and reducing activity (161.44 and 113.62 mg TE/g extract, for CUPRAC (cupric reducing antioxidant capacity) and FRAP (ferric reducing antioxidant power), respectively). SM extract exhibited the highest cholinesterase's inhibitory action (3.82 and 4.76 mg galantamine equivalent/g extract, for acetyland butyrylcholinesterase, respectively). The extracts showed higher inhibition against alpha-glucosidase (7.32-11.99 mmol acarbose equivalent [ACAE]/g extract) compared to alpha-amylase (0.51-0.55 mmol ACAE/g extract). SC extract was the most active (137.54 mg kojic acid equivalent/g extract) tyrosinase inhibitor and FV extract (113.75 mg Orlistat equivalent/g) the best lipase inhibitor. SM extract showed potent antibacterial effect against B. cereus (MIC (minimum inhibitory concentration) 0.180 mg/mL), P. mirabilis (MIC 0.180 mg/mL), M. flavus (MIC 0.560 mg/mL), P. aeruginosa (MIC 0.275 mg/mL), and S. typhimurium (MIC 1.500 mg/mL). FV extract (MIC 0.140 mg/mL) suppressed A. fumigates growth. Cytotoxicity was assessed on murine macrophage (RAW 264.7), human embryonic kidney (HEK 293), and human hepatocellular carcinoma (HepG2) cell lines. FV (60.3%) and SM (57.4%) showed the highest reduction on RAW 264.7 cellular viability, whereas SM (74.1%) showed toxicity against HepG2. This study supports that the Apiaceae species could be considered as promising candidates for the development of novel pharmacophores for the management of several human ailments.
- Shining the spotlight on NMR metabolic profiling and bioactivities of different solvent extracts of Piliostigma thonningiiPublication . Sinan, Kouadio Ibrahime; Zengin, Gokhan; Fiorentino, Antonio; Vuotto, Chiara; Mahomoodally, Mohamad Fawzi; Jugreet, Sharmeen; Glamočlija, Jasmina; Soković, Marina; Etienne, Ouattara Katinan; Pereira, Catarina Guerreiro; Custódio, Luísa; Rengasamy, Kannan R.R.; D'Abrosca, BrigidaPiliostigma thonningii (Fabaceae) is a versatile medicinal plant used as a traditional remedy, especially in African countries. In the present study, ethyl acetate, methanolic and water extracts of different parts of (fruits, leaves, stem barks) P. thonningii were evaluated for their bioactive contents, enzyme inhibitory, antioxidant and antimicrobial activities. Antioxidant potentials were also displayed in various methods like phosphomolybdenum assay (0.79-5.94 mmol TE/g), radical scavenging assays (DPPH: 20.39-471.28 mg TE/g; ABTS: 42.43-654.14 mg TE/g); reducing assays (CUPRAC: 102.98-1207.42 mg TE/g; FRAP: 45.30-656.25 mg TE/g) and metal chelating activity (2.55-22.32 mg EDTAE/g). In particular, the methanolic and water extracts showed better antioxidant potential. Except for a few, the extracts were also found to act as inhibitors of enzymes such as acetylcholinesterase (2.35-5.35 mg GALAE/g), butyrylcholinesterase (0.83-13.80 mg GALAE/g), tymsinase (4.03-143.86 mg KAE/g), amylase (0.21-1.39 mmol ACAE/g) and glucosidase (3.52-7.13 mmol ACAE/g). Additionally, all the tested extracts possessed significant antibacterial (minimum inhibitory concentration (MIC) and minimum bactericidal concentration: 0.11-0.61 mg/ml) and antifungal (MIC and minimum fungicidal concentration: 0.04-0.61 mg/ml) effects. Indeed, the data garnered from the present study provides a bright scope for the advanced investigation on this particular plant possessing pharmacologically active traits.