Browsing by Author "Szewczyk, Agnieszka"
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- Antibacterial, antioxidant and anti-proliferative properties and zinc content of five south Portugal herbsPublication . Nunes, Ricardo; Pasko, Pawel; Tyszka-Czochara, Malgorzata; Szewczyk, Agnieszka; Szlosarczyk, Marek; Saraiva de Carvalho, IsabelContext: Crataegus monogyna L. (Rosaceae) (CM), Equisetum telmateia L. (Equisataceae) (ET), Geranium purpureum Vil. (Geraniaceae) (GP), Mentha suaveolens Ehrh. (Lamiaceae) (MS), and Lavandula stoechas L. spp. luisieri (Lamiaceae) (LS) are all medicinal. Objective: To evaluate the antioxidant, antiproliferative and antimicrobial activities of plant extracts and quantify individual phenolics and zinc. Material and methods: Aerial part extracts were prepared with water (W), ethanol (E) and an 80% mixture (80EW). Antioxidant activity was measured with TAA, FRAP and RP methods. Phenolics were quantified with a HPLC. Zinc was quantified using voltammetry. Antibacterial activity (after 48 h) was tested using Enterococcus faecalis, Bacillus cereus, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Listeria monocytogenes. Antiproliferative activity (after 24 h) was tested using HEP G2 cells and fibroblasts. Results: Solvents influenced results; the best were E and 80EW. GP had the highest antioxidant activity (TAA and FRAP of 536.90mg AAE/g dw and 783.48mg TE/g dw, respectively). CM had the highest zinc concentration (37.21 mg/kg) and phenolic variety, with neochlorogenic acid as the most abundant (92.91 mg/100 g dw). LS was rich in rosmarinic acid (301.71 mg/100 g dw). GP and LS inhibited the most microorganisms: B. cereus, E. coli and S. aureus. GP also inhibited E. faecalis. CM had the lowest MIC: 5830 mu g/mL. The antibacterial activity is explained by the phenolics present. LS and CM showed the most significant anti-proliferative activity, which is explained by their zinc content. Conclusion: The most promising plants for further studies are CM, LS and GP.
- Identification of Predominant Phytochemical Compounds and Cytotoxic Activity of Wild Olive Leaves (Olea europaea L. ssp sylvestris) Harvested in South PortugalPublication . Makowska-Was, Justyna; Galanty, Agnieszka; Gdula-Argasinska, Joanna; Tyszka-Czochara, Malgorzata; Szewczyk, Agnieszka; Nunes, Ricardo; Carvalho, Isabel S.; Michalik, Marta; Pasko, PawelThis study has been aimed at providing a qualitative and quantitative evaluation of selected phytochemicals such as phenolic acids, flavonoids, oleuropein, fatty acids profile, and volatile oil compounds, present in wild olive leaves harvested in Portugal, as well as at determining their antioxidant and cytotoxic potential against human melanoma HTB-140 and WM793, prostate cancer DU-145 and PC-3, hepatocellular carcinoma Hep G2 cell lines, as well as normal human skin fibroblasts BJ and prostate epithelial cells PNT2. Gallic, protocatechuic, p-hydroxybenzoic, vanillic acids, apigenin 7-O-glucoside, luteolin 7-O-glucoside, and rutin were identified in olive leaves. The amount of oleuropein was equal to 22.64 g/kg dry weight. (E)-Anethole (32.35%), fenchone (11.89%), and (Z)-3-nonen-1-ol (8%) were found to be the main constituents of the oil volatile fraction, whereas palmitic, oleic, and alpha-linolenic acid were determined to be dominating fatty acids. Olive leaves methanol extract was observed to exerted a significant, selective cytotoxic effect on DU-145 and PC-3 cell lines. Except the essential oil composition, evaluated wild olive leaves, with regard to their quantitative and qualitative composition, do not substantially differ from the leaves of other cultivars grown for industrial purposes and they reveal considerable antioxidant and cytotoxic properties. Thus, the wild species may prove to be suitable for use in traditional medicine as cancer chemoprevention.
- In vitro biological activity screening of arbutus unedo leaves in light of their traditional usePublication . Nunes, Ricardo; Saraiva De Carvalho, Isabel; Paśko, Paweł; Tyszka – Czochara, Małgorzata; Szewczyk, Agnieszka; Szlósarczyk, Marek; Podolak, Irma; Galanty, AgnieszkaThe biological activity of A. unedo leaves, in relation to their traditional medicinal use in treating gastrointestinal and urinary tract diseases was verified. The ethanol extract of the leaves was quantitatively described in terms of flavonoids and phenolic acids (RP-HPLC), and zinc (voltametric analysis) and subjected to cytotoxic (MTT), antioxidant (TPC, TFC, TAA, RP, DPPH, and FRAP), antibacterial (disc diffusion), and antidiabetic assays (enzyme inhibition) in vitro. The extract was highly cytotoxic against prostate cancer cells, with less impact on colon carcinomas, while being nontoxic to normal prostate and kidney cells, and demonstrated antioxidant and antidiabetic activities.