Browsing by Author "Szlósarczyk, Marek"
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- Determination of essential minerals and trace elements in edible sprouts from different botanical families—application of chemometric analysisPublication . Dobrowolska-Iwanek, Justyna; Zagrodzki, Paweł; Galanty, Agnieszka; Fołta, Maria; Kryczyk-Kozioł, Jadwiga; Szlósarczyk, Marek; Rubio, Pol Salvans; Saraiva de Carvalho, Isabel; Paśko, PawełBackground: elemental deficiency may result in the malfunctioning of human organisms. Sprouts, with their attractive looks and well-established popularity, may be considered as alternative sources of elements in the diet. Moreover, the uptake of micro- and macronutrients from sprouts is better when compared to other vegetable sources. The aim of the study was to determine and compare the level of the selected essential minerals and trace elements in 25 sprouts from different botanical families, to preselect the richest species of high importance for human diets. Methods: the Cu, Zn, Mn, Fe, Mg, Ca determinations were performed using atomic absorption spectrometry with flame atomization and iodine by the colorimetric method. Results: beetroot sprouts had the highest levels of Zn, Fe, and Mg, while onion sprouts were the richest in Mn and Ca, among all of the tested sprouts. Sprouts of the Brassicaceae family were generally richer in Ca, Mg, and Zn than sprouts from the Fabaceae family. Results allow preselection of the most perspective sprouts as possible dietary sources of essential minerals and trace elements. For rucola, leeks, onions, and beetroot sprouts, the data on minerals and trace element compositions were performed for the first time.
- In vitro biological activity screening of arbutus unedo leaves in light of their traditional usePublication . Nunes, Ricardo; Saraiva De Carvalho, Isabel; Paśko, Paweł; Tyszka – Czochara, Małgorzata; Szewczyk, Agnieszka; Szlósarczyk, Marek; Podolak, Irma; Galanty, AgnieszkaThe biological activity of A. unedo leaves, in relation to their traditional medicinal use in treating gastrointestinal and urinary tract diseases was verified. The ethanol extract of the leaves was quantitatively described in terms of flavonoids and phenolic acids (RP-HPLC), and zinc (voltametric analysis) and subjected to cytotoxic (MTT), antioxidant (TPC, TFC, TAA, RP, DPPH, and FRAP), antibacterial (disc diffusion), and antidiabetic assays (enzyme inhibition) in vitro. The extract was highly cytotoxic against prostate cancer cells, with less impact on colon carcinomas, while being nontoxic to normal prostate and kidney cells, and demonstrated antioxidant and antidiabetic activities.