Browsing by Author "Vatutin, Alexey"
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- Impact of high fat diet in the gut microbiota and tissue regeneration in acomys modelPublication . Vatutin, Alexey; Faleiro, Maria Leonor; Vitorino, MartaThe increase of the Type 2 Diabetes (T2D) worldwide is of great concern, implying, at long term, the development of comorbidities, including neuropathy, nephropathy and retinopathy, and costs to treat these T2D microvascular complications are tremendous for any National Health Service. The development of T2D both in mice and humans is accompanied by dysbiosis (disturbed gut microbiota). The rodent model Acomys cahirinus has a notable capacity to regenerate several tissues, which is not observed in other mammalians. However, A. cahirinus under a High Fat Diet (HFD) can develop diabetes together with obesity. The main aim of the present study was to evaluate the alterations in specific gut bacterial groups induced by the HFD and the impact of this diet on the induction of T2D and the regenerative ability of A. cahirinus. The faecal DNA of mice was extracted using the kit QIamp PowerFecal DNA kit (Qiagen). The quantification of the bacterial groups was performed by qPCR. The monotorization of the progression of diabetes was done weekly by measuring the blood sugar levels and also by following the weight increase. When the diabetes was established in some mice from each group Control and HFD an ear punch of 4 mm diameter was done and a weekly control and measurement of the punch was monitored in order to evaluate the potential impact of the diabetes on the regenerative capacity of A. cahirinus . Only two mice fom HFD group developed T2D, and in these mice the bacterial groups did not suffered significant alterations, however in these mice the ear puch took more time to regenerate in comparison withg the ear puch performed in control group. These results evidence that the colony of A. cahirinus established at University of Algarve seems to be resistant to development of T2D. In future the use of a metagenomic approach could help understand the gut microbiota profile under a HFD that can protect this mice model from T2D. Such knowledge will help to mount strategies to help protect humans from developing T2D.