Percorrer por autor "Venanzoni, Roberto"
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- Characterising the metabolomic diversity and biological potentials of extracts from different parts of two cistus species using UHPLC-MS/MS and In vitro techniquesPublication . Ahmed, Shakeel; Zengin, Gokhan; Selvi, Selami; Ak, Gunes; Cziáky, Zoltán; Jekő, József; Rodrigues, Maria João; Custódio, Luísa; Venanzoni, Roberto; Flores, Giancarlo Angeles; Cusumano, Gaia; Angelini, PaolaThis study investigates the biochemical composition and biological properties of different parts (leaves, roots, and twigs) of two Cistus species (Cistus monspeliasis and Cistus parviflorus). The extracts were analysed using UHPLC-MS/MS to determine their chemical profiling. A range of antioxidant assays were performed to evaluate the extract’s antioxidant capabilities. The enzyme inhibition studies focused on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-amylase, and α-glucosidase and tyrosinase. In addition, the study examined the antimicrobial effects on different bacteria and yeasts and evaluated the toxicity using the MTT assay. Quinic acid, citric acid, gallic acid, catechin, quercetin derivatives, kaempferol, myricetin, ellagic acid, prodelphinidins, procyanidins, scopoletin, and flavogallonic acid dilactone are the main bioactive compounds found in both species. In enzyme inhibition assays, C. monspeliasis roots exhibited significant activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), with the values of 2.58 ± 0.02 mg GALAE/g and 11.37 ± 1.93 mg GALAE/g, respectively. Cytotoxicity studies showed mostly weak toxicity, with some samples moderately reducing viability in RAW and HepG2 cells. These findings underscore the diverse biochemical profiles and bioactive potential of Cistus species, suggesting their utility as natural sources of antioxidants and enzyme inhibitors for pharmaceutical and nutraceutical development.
- A multidirectional study on chemical fingerprints and biological activities of three Cistus extracts (C. creticus, C. laurifolius, and C. salviifolius) with ethnomedicinal usesPublication . Yagi, Sakina; Zengin, Gökhan; SELVİ, Selami; AK, Güneş; CZIÁKY, Zoltán; Jekő, József; Rodrigues, Maria João; Custódio, Luísa; Venanzoni, Roberto; Flores, Giancarlo Angeles; Cusumano, Gaia; ANGELINI, PaolaHumans have used medicinal plants to treat various diseases for thousands of years. Cistus species are also widely used in traditional medicine and have various medicinal applications; therefore, they deserve more in-depth research. The present study evaluated the chemical profile, antioxidant, enzyme inhibition, and cytotoxic properties of the twigs and leaves of C. creticus L., C. laurifolius L., and C. salviifolius L. grown in Türkiye. The methanolic extracts of the three species were rich in phenolics, mainly flavonoids. Exerted potent antioxidant activity with a methanolic extract from the leaves of C. salviifolius displayed the highest total phenolic (97.08-mg gallic acid equivalent/g) and flavonoid (49.60-mg rutin equivalent/g) contents, as well as antiradical (2,2-diphenyl-1-picrylhydrazyl) assay = 612.11 mg TE (trolox equivalent)/g; (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) = 804.66 mg TE/g), reducing ions (cupric reducing antioxidant capacity) = 690.54 mg TE/g; ferric reducing antioxidant power = 459.34 mg TE/g), and chelating (15.58 mg EDTAE/g) properties. It also revealed the best amylase and glucosidase inhibitory activity. Extracts from the twigs of the three Cistus species, except the leaves of C. salviifolius and C. laurifolius, displayed comparable acetylcholinesterase inhibitory activity (2.48–2.57 mg galanthamine equivalent (GALAE)/g). The twig of C. laurifolius also exerted the best antibutyrylcholinesterase (10.50 mg GALAE/g) and antityrosinase (73.15 mg kojic acid equivalent/g) activities. C. creticus leaves revealed toxicity toward the RAW cell line (cell viability reduced to 68.8%) and were not toxic to normal cells (S17). In conclusion, these three Cistus species were shown to be a rich source of bioactive compounds with the potential for future applications in the food, pharmaceutical, and cosmetic industries.