Browsing by Issue Date, starting with "2023-05-09"
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- Requalificação de frentes ribeirinhas urbanas em sistemas lagunares: o caso de OlhãoPublication . Carvalho, Miguel; Batista, Desidério; Santos, AméliaEste relatório de estágio, aborda intervenções no espaço público, em frentes ribeirinhas ou espaços de cidades que se encontram em interface entre a cidade e os sistemas lagunares, mais concretamente, cidades que confrontam com zonas húmidas, inseridos em biomas de sapais. Serão analisados 3 casos de requalificações ribeirinhas, com características idênticas, ao nível do clima, da cultura e da escala. Será descrito o caso de Huelva, na sua requalificação do “Paseo de la Ría” e o caso de Aveiro, na sua requalificação do “jardim do rossio”. Estudou-se estes exemplos, como forma de abordagem e comparação, com o caso desenvolvido, que contou com a participação do autor do relatório, que é o caso da “Requalificação da Frente Ribeirinha de Olhão”. Por fim, analisaram-se os 3 casos, de forma comparativa e conclusiva.
- mef2ca and mef2cb Double mutant Zebrafish show altered craniofacial phenotype and motor behaviourPublication . Adrião, Andreia; Mariano, Sara; Mariano, José; Gavaia, Paulo; Cancela, M. Leonor; Vitorino, Marta; Conceição, NatérciaThe transcription factor MEF2C is crucial in neuronal, cardiac, bone and cartilage molecular processes, as well as for craniofacial development. MEF2C was associated with the human disease MRD20, whose patients show abnormal neuronal and craniofacial development. Zebrafish mef2ca;mef2cb double mutants were analysed for abnormalities in craniofacial and behaviour development through phenotypic analysis. Quantitative PCR was performed to investigate the expression levels of neuronal marker genes in mutant larvae. The motor behaviour was analysed by the swimming activity of 6 dpf larvae. We found that mef2ca;mef2cb double mutants display several abnormal phenotypes during early development, including those already described in zebrafish carrying mutations in each paralog, but also (i) a severe craniofacial phenotype (comprising both cartilaginous and dermal bone structures), (ii) developmental arrest due to the disruption of cardiac oedema and (iii) clear alterations in behaviour. We demonstrate that the defects observed in zebrafish mef2ca;mef2cb double mutants are similar to those previously described in MEF2C-null mice and MRD20 patients, confirming the usefulness of these mutant lines as a model for studies concerning MRD20 disease, the identification of new therapeutic targets and screening for possible rescue strategies.
- A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomiPublication . Neves, Dina; Figueiredo, Andreia; Maia, Marisa; Laczko, Endre; Pais, Maria Salomé; Cravador, AlfredoPhlomis purpurea grows spontaneously in the southern Iberian Peninsula, namely in cork oak (Quercus suber) forests. In a previous transcriptome analysis, we reported on its immunity against Phytophthora cinnamomi. However, little is known about the involvement of secondary metabolites in the P. purpurea defense response. It is known, though, that root exudates are toxic to this pathogen. To understand the involvement of secondary metabolites in the defense of P. purpurea, a metabolome analysis was performed using the leaves and roots of plants challenged with the pathogen for over 72 h. The putatively identified compounds were constitutively produced. Alkaloids, fatty acids, flavonoids, glucosinolates, polyketides, prenol lipids, phenylpropanoids, sterols, and terpenoids were differentially produced in these leaves and roots along the experiment timescale. It must be emphasized that the constitutive production of taurine in leaves and its increase soon after challenging suggests its role in P. purpurea immunity against the stress imposed by the oomycete. The rapid increase in secondary metabolite production by this plant species accounts for a concerted action of multiple compounds and genes on the innate protection of Phlomis purpurea against Phytophthora cinnamomi. The combination of the metabolome with the transcriptome data previously disclosed confirms the mentioned innate immunity of this plant against a devastating pathogen. It suggests its potential as an antagonist in phytopathogens’ biological control. Its application in green forestry/agriculture is therefore possible.
- A metabolome analysis and the immunity of Phlomis purpurea against Phytophthora cinnamomiPublication . Neves, Dina; Figueiredo, Andreia; Maia, Marisa; Laczko, Endre; Pais, Maria Salomé; Cravador, AlfredoPhlomis purpurea grows spontaneously in the southern Iberian Peninsula, namely in cork oak (Quercus suber) forests. In a previous transcriptome analysis, we reported on its immunity against Phytophthora cinnamomi. However, little is known about the involvement of secondary metabolites in the P. purpurea defense response. It is known, though, that root exudates are toxic to this pathogen. To understand the involvement of secondary metabolites in the defense of P. purpurea, a metabolome analysis was performed using the leaves and roots of plants challenged with the pathogen for over 72 h. The putatively identified compounds were constitutively produced. Alkaloids, fatty acids, flavonoids, glucosinolates, polyketides, prenol lipids, phenylpropanoids, sterols, and terpenoids were differentially produced in these leaves and roots along the experiment timescale. It must be emphasized that the constitutive production of taurine in leaves and its increase soon after challenging suggests its role in P. purpurea immunity against the stress imposed by the oomycete. The rapid increase in secondary metabolite production by this plant species accounts for a concerted action of multiple compounds and genes on the innate protection of Phlomis purpurea against Phytophthora cinnamomi. The combination of the metabolome with the transcriptome data previously disclosed confirms the mentioned innate immunity of this plant against a devastating pathogen. It suggests its potential as an antagonist in phytopathogens’ biological control. Its application in green forestry/agriculture is therefore possible.