Browsing by Issue Date, starting with "2024-11-23"
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- Quintal dos sons: a bridge between Porto and the Vale do JequitinhonhaPublication . Sapucaia Rocha Rocha, RosimáriaThe article describes a sound installation developed in the doctoral in Digital Media Art (2022). "Quintal dos Sons" aims to reflect on contemporary sounds, inviting participants to interact with sounds triggered by proximity sensors. The research combines artistic practice and theoretical investigation, using the Arts-Based Research methodology. The sounds in the installation reflect the concept of Soundscapes, encompassing sounds of nature and sounds produced by humans, exploring the extremes of contemporary life. In the development of artistic work, we include sound mapping and observation of urban spaces, using technology in recording soundscapes and constructingthe artifact. The sonic narrative spans from the first sounds of human birth to the connection with nature, urban noises, and traditional songs of the Jequitinhonha Valley. The metaphor of the backyard and the image of the bridge, like Porto-Portugal and Almenara-Vale do Jequitinhonha-MG, are crucial elements in the creative process, reflecting the life of the author and the communities. The article concludes by emphasizing the importance of musical activity as inherently human, associating geographical experiences with cultural and physical heritage, and serving as a test substrate for scientific-artistic sound activities.
- Transcriptional regulation of the Human MGP promoter: Identification of downstream repressorsPublication . Caiado, Helena; Cancela, M. Leonor; Conceição, NatérciaMatrix Gla protein (MGP) is a vitamin K-dependent γ-carboxylated protein that was initially identified as a physiological inhibitor of ectopic calcification, primarily affecting cartilage and the vascular system. Mutations in the MGP gene were found to be responsible for the Keutel syndrome, a condition characterized by abnormal calcifications in the cartilage, lungs, brain, and vascular system. MGP has been shown to be dysregulated in several tumors, including cervical, ovarian, urogenital, and breast cancers. Using bioinformatic approaches, transcription factor binding sites (TFBSs) containing CpG dinucleotides were identified in the MGP promoter, including those for YY1, GATA1, and C/EBPα. We carried out functional tests using transient transfections with a luciferase reporter assay, primarily for the transcription factors YY1, GATA1, C/EBPα, and RUNX2. By co-transfection analysis, we found that YY1, GATA1, and C/EBPα repressed the MGP promoter. Furthermore, the co-transfection with RUNX2 activated the MGP promoter. In addition, MGP expression is negatively or positively correlated with the studied TFs’ expression levels in several cancer types. This study provides novel insights into MGP regulation by demonstrating that YY1, GATA1, and C/EBPα are negative regulators of the MGP promoter, and DNA methylation may influence their activity. The dysregulation of these mechanisms in cancer should be further elucidated.