Browsing by Issue Date, starting with "2025-05-20"
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- Effects of a land and aquatic exercise-based program on pain, mobility and quality of life in patients with chronic low back pain: a study protocol for a randomized controlled trialPublication . Borges, Joana; Monteiro, Diogo; Silva, Manuela Fernanda Gomes Moreira da; Jacinto, Miguel; Pastilha, Tiago; Duarte-Mendes, Pedro; Shabnam ShahAliChronic low back pain (CLBP) is a disease with significant functional, emotional and social impact. Several interventions are proposed for its management and exercise is one of main, land-based or water-based. This study describes a randomized controlled trial that will analyze the effect of a combined aquatic and land-based exercise program compared to an aquatic-based program on pain, functional incapacity and quality of life in adults with CLBP. Additionally, it will analyze the effects of exercise cessation in the same outcomes.
- O papel moderador dos estilos de vinculação e da vergonha interiorizada na relação entre disponibilidade emocional parental e o abuso de substânciasPublication . Custódio, Joana; Giger, JeanO consumo de substâncias psicoativas tem um grande impacto na saúde geral da população, como tal, é necessário compreender de uma forma mais abrangente este comportamento, para que seja possível prevenir e intervir. Este estudo pretende investigar a existência de uma relação entre a disponibilidade emocional percecionada com os consumos, como também, averiguar se os estilos de vinculação nas relações interpessoais e a vergonha internalizada são fatores de risco para o aumento dos mesmos. Para tal, 231 participantes responderam a questionários sobre a disponibilidade emocional percecionada, vergonha internalizada, estilos de vinculação, e motivos para os consumos. Os resultados refletem correlações estatisticamente significativas positivas entre as variáveis disponibilidade emocional dos pais percecionada e estilos de vinculação mais confiantes e confortáveis. Por outro lado, também foi possível observar correlações estatisticamente significativas negativas entre a disponibilidade emocional dos pais percecionada, vergonha internalizada, estilos de vinculação ansiosos e motivos para o consumo de substâncias. Estes resultados sugerem que quanto menos disponibilidade emocional percecionada mais estratégias de coping mal adaptativas irão ser desenvolvidas pelo individuo, e este terá uma maior predisposição para recorrer aos consumos como forma de aliviar sofrimento psíquico.
- The BiP Chaperone from the Endoplasmic Reticulum of Zebrafish (Daniorerio) and Golden Hamster (Mesocricetus auratus)Publication . Roszik, Lilla; Melo, Eduardo PinhoThe Hsp70 family of molecular chaperones plays a crucial role in maintaining cellular proteostasis by promoting protein folding, transport, and degradation. One of its component proteins, the binding immunoglobulin protein (BiP), is an essential endoplasmic reticulum chaperone required for proper protein folding and stress response. The primary objective of this work was to clone the open reading frame of the BiP gene from zebrafish (Danio rerio), a model organism that is frequently used to study the molecular and genetic causes of human disorders. More than 70% of the genes between humans and zebrafish are similar, including many orthologs connected to neurodegenerative illnesses like Alzheimer's. For the sake of further functional investigations, the open reading frame of the zebrafish BiP gene was amplified and inserted into a bacterial expression vector. From zebrafish, the total RNA of caudal cell lines was extracted and reverse transcribed to create complementary DNA. The target DNA sequence was amplified by polymerase chain reaction (PCR) using specific primers for BiP. The golden hamster BiP ORF was removed from the backbone pQE10, and the amplified product was cloned into the vector. After transforming the recombinant plasmid into Escherichia coli DH5α for propagation and isolation, the insertion of the open reading frame of zebrafish BiP was sent to sequencing. Unfortunately, E coli recombines the zebrafish BiP ORF, contrary to the golden hamster ORF previously cloned into the same vector. Different E coli strains deficient in DNA recombination should be tested soon. This work aimed to establish the foundation for further investigations into the activity and biochemical characteristics of zebrafish BiP and its interactions with co-chaperones and client proteins in comparison with the mammalian BiP from golden hamster. At the longer term, we want to understand how the chaperone machinery of the endoplasmic reticulum has evolved in resolving protein aggregates associated with neurodegenerative diseases.
- Tri-Collab: a machine learning project to leverage innovation ecosystems in PortugalPublication . Marujo, Ângelo; Afonso, Bruno; Martins, Inês; Pires, Lisandro; Fernandes, Silvia C. Pinto de BritoThis project consists of a digital platform named Tri-Collab, where investors, entrepreneurs, and other agents (mainly talents) can cooperate on their ideas and eventually co-create. It is a digital means for this triad of actors (among other potential ones) to better adjust their requirements. It includes an app that easily communicates with a database of projects, innovation agents and their profiles, and the originality lies in the matching algorithm. Thus, co-creation can have better support through this assertive interconnection of players and their resources. This work also highlights the usefulness of the Canvas Business Model in structuring the idea and its dashboard, allowing a comprehensive view of channels, challenges and gains. Also, the potential of machine learning in improving matchmaking platforms is discussed, especially when technological advancements allow for forecasts and match people at scale.
- The early–middle pleistocene transition in the gulf of Cadiz (NE Atlantic) – an interplay between subtropical gyre and extremely cold surface watersPublication . Mega, Aline; Rodrigues, Teresa; Salgueiro, Emilia; Padilha, Mária; Kuhnert, Henning; Voelker, AntjeBesides the shift in dominant orbital cyclicity depicted in paleoclimate proxy records, the Mid-Pleistocene Transition or Early-Middle Pleistocene Transition (EMPT) was linked to a change in the deep thermohaline circulation. Those changes contributed to more intense and longer-lasting glacial periods and cooler sea surface temperatures (SSTs) during glacials. Within the Atlantic Ocean, the Iberian Margin is considered a key location to study climatic variations influenced by both high- and low-latitude processes. In this study we focus on IODP Site U1387 on the southern Portuguese margin to reconstruct surface water circulation changes and related plankton foraminifera ecosystems during the interval of Marine Isotope Stage (MIS) 28 to MIS 18 (1006-750 ka). Our planktonic foraminifera assemblages and SST reconstructions (foraminifera assemblages and U-37(K ') alkenone index) demonstrate warm, relative stable SST conditions during much of the interval due to persistent influence of subtropical gyre waters as indicated by the tropical-subtropical and Azores Current-related foraminifera species and the periods with dominant sinistral coiling direction of the species Globorotalia truncatulinoides. Maximum interglacial SSTs were up to 2 degrees C warmer than at present in both summer and winter, with the exception of interglacial MIS 23 with SSTs similar to 1.5 degrees C colder than in the other interglacials. Subsequent to the respective glacial inception, the relatively warm conditions were periodically interrupted by millennial-scale extreme cold events when polar species Neogloboquadrina pachyderma became abundant (> 30 %), and the SSTs, reconstructed from the foraminifera assemblage data, dropped below 10 degrees C in summer and 5 degrees C in winter, although some of those values might be overestimated. The most pronounced event, considering the amplitude of cooling and duration, occurred between 870 and 864 ka, marking the terminal stadial event of the MIS 22-MIS 21 transition (Termination X). Extreme cold events, always associated with the incursion of subpolar waters into the Gulf of Cadiz, mark all the terminal stadial events from Terminations XII to IX and the millennial-scale variability during the transitions to full glacial conditions, although the duration of the cooling varied greatly. The extreme cooling was only possible through migration of the subarctic front into the lower mid-latitudes as a consequence of cooling and freshening in the higher latitudes and the associated extreme reduction in the Atlantic Meridional Overturning Circulation. The amplitude of cooling, duration, and frequency of subpolar water incursions during MIS 24 to MIS 22 stands out, providing further evidence for the "900 ka event" being a key feature of the EMPT.