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  • Mixed-valence pentadecavanadate with Ca2+-ATPase inhibition potential and anti-breast cancer activity
    Publication . Brito, Bianca R.; Camilo, Heloísa de S.; Cruz, Anderson F. da; Ribeiro, Ronny R.; Sá, Eduardo L. de; Oliveira, Carolina Camargo de; Fraqueza, Gil; Klassen, Giseli; Aureliano, Manuel; Nunes, Giovana G.
    Polyoxovanadates are a subclass of polyoxometalates (POMs) known to interact with proteins and to present anticancer, antimicrobial, and antiviral activities. Herein, we aimed to pursue the study of the breast anticancer activity of a mixed-valence polyoxovanadate, [Cl@VV 7V IV 8O36] 6− (V15) against MCF-7 and MDA-MB-231 cancer cell lines and to analyze its Ca2+-ATPase inhibition potential. 51V NMR and UV-Vis/NIR studies of V15 indicated its stability in HEPES and RPMI media. For the Ca2+-ATPase activity, V15 showed an IC50 value of 14.2 µM and a mixed type of inhibition. The electrostatic potential map of V15 and other POMs were correlated with the enzyme activity inhibition. V15 also exhibited cytotoxicity against MDA-MB-231 (IC50 = 17.2 µM) and MCF-7 (IC50 = 15.1 µM) breast cancer cell lines. Using V15 concentrations equivalent to half and 1/4 of the IC50, it was observed that MDA-MB-231 cell migration was reduced by 90 and 70%, after 24 h, respectively. Moreover, V15 caused morphological changes from fusiform to an epitheliallike (amoeboid) shape. Finally, V15 induced the increase in RIPK1, MLKL, and RIPK3 gene expression, up to 3, 10, and 15-fold, respectively, pointing out that the mechanisms of cell death in the triple-negative breast cancer cell line may occur by necroptosis.
    2025-09-12Journal article Open access Show more
  • Smart specialisation evaluation with real project data: a general equilibrium model in Portugal’s Centro region
    Publication . Szabó, Norbert; Polónyi-Andor, Krisztina; Sebestyén, Tamás; Pinto, Hugo
    As the first smart specialisation planning period (2014–2020) ended, monitoring and evaluation became crucial. Analysing the successes and failures of smart specialisation strategy (S3) through a good monitoring and evaluation system contributes to the evidence base on which an efficient redesign and improvement of S3 can be performed. In this paper, we apply an extended version of the geographic, macro and regional (GMR)-Europe economic impact model to provide a real-life evaluation of the implementation of S3 in Centro region using cohesion policy fund data between 2014 and 2023. Simulations reveal that policy instruments and policy mixes of priorities differ in efficiency and identify where improvements are desired.
    2025-09-12Journal article Open access Show more