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- Unravelling the chemical nature of the spawning-inducing pheromone (SIP) in the pacific oyster (Magallana gigas)Publication . Rato, Ana Cláudia Nunes; sandra, Joaquim; Matias, Domitília; Hubbard, PeterIn external fertilisation, spawning synchrony is often mediated by pheromones. However, their chemical nature is rarely well-established; this is particularly true for bivalves. This study used an electrophysiological technique—the electro-osphradiogram (EOsG)—to investigate the spawning-inducing pheromone (SIP) in the Pacific oyster (Magallana gigas), a species of economic and environmental relevance. Recording the electrophysiological response of the osphradium to conspecific sperm milt and its fractions, we show that the SIP is multicomponent, likely proteinaceous—with at least one component linked to the spermatozoa and the other components in free solution—and all resistant to freezing. At least three active components are involved: one of about 35 kDa, one between 3 and 10 kDa and one of less than 3 kDa. All three, alone, evoke responses from the osphradium, but all three must probably be present to evoke the full biological response—gamete release— in the receiver. All three are likely polar; none were retained by a range of solid-phase extraction cartridges. We suggest that the EOsG will be useful to isolate and identify the individual components of the oyster SIP. Successful identification of the SIP will represent an important step towards more sustainable and efficient bivalve hatchery practices.
- Unravelling the chemical nature of the spawning-inducing pheromone (SIP) in the pacific oyster (Magallana gigas)Publication . Rato, Ana Cláudia Nunes; Joaquim, Sandra; Matias, Domitília; Hubbard, PeterIn external fertilisation, spawning synchrony is often mediated by pheromones. However, their chemical nature is rarely well-established; this is particularly true for bivalves. This study used an electrophysiological technique—the electro-osphradiogram (EOsG)—to investigate the spawning-inducing pheromone (SIP) in the Pacific oyster (Magallana gigas), a species of economic and environmental relevance. Recording the electrophysiological response of the osphradium to conspecific sperm milt and its fractions, we show that the SIP is multicomponent, likely proteinaceous—with at least one component linked to the spermatozoa and the other components in free solution—and all resistant to freezing. At least three active components are involved: one of about 35 kDa, one between 3 and 10 kDa and one of less than 3 kDa. All three, alone, evoke responses from the osphradium, but all three must probably be present to evoke the full biological response—gamete release— in the receiver. All three are likely polar; none were retained by a range of solid-phase extraction cartridges. We suggest that the EOsG will be useful to isolate and identify the individual components of the oyster SIP. Successful identification of the SIP will represent an important step towards more sustainable and efficient bivalve hatchery practices.
- From cues to engagement: a comprehensive survey and holistic architecture for computer vision-based audience analysis in live eventsPublication . Lemos, Marco; Cardoso, Pedro; Rodrigues, JoaoThe accurate measurement of audience engagement in real-world live events remains a significant challenge, with the majority of existing research confined to controlled environments like classrooms. This paper presents a comprehensive survey of Computer Vision AI-driven methods for real-time audience engagement monitoring and proposes a novel, holistic architecture to address this gap, with this architecture being the main contribution of the paper. The paper identifies and defines five core constructs essential for a robust analysis: Attention, Emotion and Sentiment, Body Language, Scene Dynamics, and Behaviours. Through a selective review of state-of-the-art techniques for each construct, the necessity of a multimodal approach that surpasses the limitations of isolated indicators is highlighted. The work synthesises a fragmented field into a unified taxonomy and introduces a modular architecture that integrates these constructs with practical, businessoriented metrics such as Commitment, Conversion, and Retention. Finally, by integrating cognitive, affective, and behavioural signals, this work provides a roadmap for developing operational systems that can transform live event experience and management through data-driven, real-time analytics.
- From cues to engagement: a comprehensive survey and holistic architecture for computer vision-based audience analysis in live eventsPublication . Lemos, Marco; Cardoso, Pedro; Rodrigues, JoaoThe accurate measurement of audience engagement in real-world live events remains a significant challenge, with the majority of existing research confined to controlled environments like classrooms. This paper presents a comprehensive survey of Computer Vision AI-driven methods for real-time audience engagement monitoring and proposes a novel, holistic architecture to address this gap, with this architecture being the main contribution of the paper. The paper identifies and defines five core constructs essential for a robust analysis: Attention, Emotion and Sentiment, Body Language, Scene Dynamics, and Behaviours. Through a selective review of state-of-the-art techniques for each construct, the necessity of a multimodal approach that surpasses the limitations of isolated indicators is highlighted. The work synthesises a fragmented field into a unified taxonomy and introduces a modular architecture that integrates these constructs with practical, businessoriented metrics such as Commitment, Conversion, and Retention. Finally, by integrating cognitive, affective, and behavioural signals, this work provides a roadmap for developing operational systems that can transform live event experience and management through data-driven, real-time analytics.